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Fang, X., Zhou, J., Liu, W., Duan, X., Gala, U., Sandoval, H., Jaiswal, M., Tong, C. (2016). Dynamin Regulates Autophagy by Modulating Lysosomal Function.  J. Genet. Genomics 43(2): 77--86.
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FBrf0231081
Publication Type
Research paper
Abstract

Autophagy is a central lysosomal degradation pathway required for maintaining cellular homeostasis and its dysfunction is associated with numerous human diseases. To identify players in autophagy, we tested ∼1200 chemically induced mutations on the X chromosome in Drosophila fat body clones and discovered that shibire (shi) plays an essential role in starvation-induced autophagy. shi encodes a dynamin protein required for fission of clathrin-coated vesicles from the plasma membrane during endocytosis. We showed that Shi is dispensable for autophagy initiation and autophagosome-lysosome fusion, but required for lysosomal/autolysosomal acidification. We also showed that other endocytic core machinery components like clathrin and AP2 play similar but not identical roles in regulating autophagy and lysosomal function as dynamin. Previous studies suggested that dynamin directly regulates autophagosome formation and autophagic lysosome reformation (ALR) through its excision activity. Here, we provide evidence that dynamin also regulates autophagy indirectly by regulating lysosomal function.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Genet. Genomics
    Title
    Journal of Genetics and Genomics [Yi chuan xue bao]
    Publication Year
    2007--
    ISBN/ISSN
    1673-8527
    Data From Reference
    Aberrations (3)
    Alleles (8)
    Genes (8)
    Human Disease Models (1)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (5)