Open Close
Reference
Citation
Zheng, J., Gao, M., Huynh, N., Tindell, S.J., Vo, H.D., McDonald, W.H., Arkov, A.L. (2016). In vivo mapping of a dynamic ribonucleoprotein granule interactome in early Drosophila embryos.  FEBS Open Bio 6(12): 1248--1256.
FlyBase ID
FBrf0234826
Publication Type
Research paper
Abstract

Macromolecular complexes and organelles play crucial roles within cells, but their native architectures are often unknown. Here, we use an evolutionarily conserved germline organelle, the germ granule, as a paradigm. In Drosophila embryos, we map one of its interactomes using a novel in vivo crosslinking approach that employs two interacting granule proteins and determines their common neighbor molecules. We identified an in vivo granule assembly of Tudor, Aubergine, motor and metabolic proteins, and RNA helicases, and provide evidence for direct interactions within this assembly using purified components. Our study indicates that germ granules contain efficient biochemical reactors involved in post-transcriptional gene regulation.

PubMed ID
PubMed Central ID
PMC5302063 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    FEBS Open Bio
    Title
    FEBS open bio
    ISBN/ISSN
    2211-5463
    Data From Reference
    Alleles (3)
    Genes (10)
    Physical Interactions (18)
    Cell Lines (1)
    Experimental Tools (2)
    Transgenic Constructs (3)