Open Close
Reference
Citation
Jin, H.S., Lee, H.M., Lee, D.H., Cha, G.H., Cho, K.S., Jang, J., Jo, E.K. (2017). Functional characterisation of the Drosophila cg6568 gene in host defence against Mycobacterium marinum.  Microbes Infect. 19(6): 351--357.
FlyBase ID
FBrf0235694
Publication Type
Research paper
Abstract

Mycobacterium marinum is a pathogenic mycobacterial species closely related to Mycobacterium tuberculosis. In this study, we established a mycobacterial infection model of Drosophila melanogaster to characterize the role played by cg6568, a homolog of the human cathelicidin gene, in the innate defense against infection. Drosophila cg6568 was expressed at various levels during all developmental stages, and the expression levels were modulated by M. marinum in a time-dependent manner. 20-hydroxyecdysone induced Drosophila cg6568 transcription both in vitro and in vivo. Using flies expressing cg6568 RNAi, we found that cg6568 was essential both for D. melanogaster survival and the exertion of antimicrobial effects during M. marinum infection. Thus, we named the gene product a cathelicidin-like antimicrobial protein of D. melanogaster (dCAMP). Our results indicate that dCAMP is crucial in terms of the innate D. melanogaster defense during M. marinum infection.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Microbes Infect.
    Title
    Microbes and Infection
    Publication Year
    1999-
    ISBN/ISSN
    1286-4579
    Data From Reference
    Alleles (4)
    Genes (2)
    Human Disease Models (1)
    Transgenic Constructs (4)