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Citation
Pereira, A., Paro, R. (2017). Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus.  Epigenetics Chromatin 10(1): 57.
FlyBase ID
FBrf0237429
Publication Type
Research paper
Abstract

Numerous target genes of the Polycomb group (PcG) are transiently activated by a stimulus and subsequently repressed. However, mechanisms by which PcG proteins regulate such target genes remain elusive. We employed the heat shock-responsive hsp70 locus in Drosophila to study the chromatin dynamics of PRC1 and its interplay with known regulators of the locus before, during and after heat shock. We detected mutually exclusive binding patterns for HSF and PRC1 at the hsp70 locus. We found that Pleiohomeotic (Pho), a DNA-binding PcG member, dynamically interacts with Spt5, an elongation factor. The dynamic interaction switch between Pho and Spt5 is triggered by the recruitment of HSF to chromatin. Mutation in the protein-protein interaction domain (REPO domain) of Pho interferes with the dynamics of its interaction with Spt5. The transcriptional kinetics of the heat shock response is negatively affected by a mutation in the REPO domain of Pho. We propose that a dynamic interaction switch between PcG proteins and an elongation factor enables stress-inducible genes to efficiently switch between ON/OFF states in the presence/absence of the activating stimulus.

PubMed ID
PubMed Central ID
PMC5718073 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Epigenetics Chromatin
    Title
    Epigenetics & chromatin
    ISBN/ISSN
    1756-8935
    Data From Reference
    Genes (14)
    Physical Interactions (3)
    Cell Lines (1)