Open Close
Jenkins, B.V., Saunders, H.A.J., Record, H.L., Johnson-Schlitz, D.M., Wildonger, J. (2017). Effects of mutating α-tubulin lysine 40 on sensory dendrite development.  J. Cell Sci. 130(24): 4120--4131.
FlyBase ID
Publication Type
Research paper

Microtubules are essential for neuronal structure and function. Axonal and dendritic microtubules are enriched in post-translational modifications that impact microtubule dynamics, transport and microtubule-associated proteins. Acetylation of α-tubulin lysine 40 (K40) is a prominent and conserved modification of neuronal microtubules. However, the cellular role of microtubule acetylation remains controversial. To resolve how microtubule acetylation might affect neuronal morphogenesis, we mutated endogenous α-tubulin in vivo using a new Drosophila strain that facilitates the rapid knock-in of designer αTub84B alleles (the predominant α-tubulin-encoding gene in flies). Leveraging our new strain, we found that microtubule acetylation, as well as polyglutamylation and (de)tyrosination, is not essential for survival. However, we found that dendrite branch refinement in sensory neurons relies on α-tubulin K40. Mutagenesis of K40 reveals moderate yet significant changes in dendritic lysosome transport, microtubule polymerization and Futsch protein distribution in dendrites but not in axons. Our studies point to an unappreciated role for α-tubulin K40 and acetylation in dendrite morphogenesis. While our results are consistent with the idea that acetylation tunes microtubule function within neurons, they also suggest there may be an acetylation-independent requirement for α-tubulin K40.This article has an associated First Person interview with the first author of the paper.

PubMed ID
PubMed Central ID
PMC5769580 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for alphaTub84B and alphaTub84D alleles.
Wildonger, 2019.6.18, Location data for alphaTub84B and alphaTub84D alleles. [FBrf0242745]

Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    J. Cell Sci.
    Journal of Cell Science
    Publication Year
    Data From Reference