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Citation
Yan, S., Acharya, S., Gröning, S., Großhans, J. (2017). Slam protein dictates subcellular localization and translation of its own mRNA.  PLoS Biol. 15(12): e2003315.
FlyBase ID
FBrf0237626
Publication Type
Research paper
Abstract

Many mRNAs specifically localize within the cytoplasm and are present in RNA-protein complexes. It is generally assumed that localization and complex formation of these RNAs are controlled by trans-acting proteins encoded by genes different than the RNAs themselves. Here, we analyze slow as molasses (slam) mRNA that prominently colocalizes with its encoded protein at the basal cortical compartment during cellularization. The functional implications of this striking colocalization have been unknown. Here, we show that slam mRNA translation is spatiotemporally controlled. We found that translation was largely restricted to the onset of cellularization when Slam protein levels at the basal domain sharply increase. slam mRNA was translated locally, at least partially, as not yet translated mRNA transiently accumulated at the basal region. Slam RNA accumulated at the basal domain only if Slam protein was present. Furthermore, a slam RNA with impaired localization but full coding capacity was only weakly translated. We detected a biochemical interaction of slam mRNA and protein as demonstrated by specific co-immunoprecipitation from embryonic lysate. The intimate relationship of slam mRNA and protein may constitute a positive feedback loop that facilitates and controls timely and rapid accumulation of Slam protein at the prospective basal region.

PubMed ID
PubMed Central ID
PMC5730382 (PMC) (EuropePMC)
Related Publication(s)
Research paper

Localization and translation control of slam in Drosophila cellularization.
Yan and Großhans, 2018, Fly 12(3-4): 191--198 [FBrf0241622]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Biol.
    Title
    PLoS Biology
    Publication Year
    2003-
    ISBN/ISSN
    1545-7885 1544-9173
    Data From Reference
    Aberrations (1)
    Alleles (9)
    Genes (8)
    Physical Interactions (1)
    Cell Lines (1)
    Natural transposons (2)
    Experimental Tools (6)
    Transgenic Constructs (9)