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Citation
Lee, P.T., Lin, G., Lin, W.W., Diao, F., White, B.H., Bellen, H.J. (2018). A kinase-dependent feedforward loop affects CREBB stability and long term memory formation.  eLife 7(): e33007.
FlyBase ID
FBrf0238210
Publication Type
Research paper
Abstract

In Drosophila, long-term memory (LTM) requires the cAMP-dependent transcription factor CREBB, expressed in the mushroom bodies (MB) and phosphorylated by PKA. To identify other kinases required for memory formation, we integrated Trojan exons encoding T2A-GAL4 into genes encoding putative kinases and selected for genes expressed in MB. These lines were screened for learning/memory deficits using UAS-RNAi knockdown based on an olfactory aversive conditioning assay. We identified a novel, conserved kinase, Meng-Po (MP, CG11221, SBK1 in human), the loss of which severely affects 3 hr memory and 24 hr LTM, but not learning. Remarkably, memory is lost upon removal of the MP protein in adult MB but restored upon its reintroduction. Overexpression of MP in MB significantly increases LTM in wild-type flies showing that MP is a limiting factor for LTM. We show that PKA phosphorylates MP and that both proteins synergize in a feedforward loop to control CREBB levels and LTM. key words: Drosophila, Mushroom bodies, SBK1, deGradFP, T2A-GAL4, MiMIC.

PubMed ID
PubMed Central ID
PMC5825208 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (62)
    Genes (32)
    Cell Lines (1)
    Natural transposons (2)
    Insertions (41)
    Experimental Tools (2)
    Transgenic Constructs (24)
    Transcripts (27)