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Shlyakhover, E., Shklyar, B., Hakim-Mishnaevski, K., Levy-Adam, F., Kurant, E. (2018). Drosophila GATA Factor Serpent Establishes Phagocytic Ability of Embryonic Macrophages.  Front. Immunol. 9(): 266.
FlyBase ID
FBrf0238417
Publication Type
Research paper
Abstract

During Drosophila embryogenesis, a large number of apoptotic cells are efficiently engulfed and degraded by professional phagocytes, macrophages. Phagocytic receptors Six-Microns-Under (SIMU), Draper (Drpr) and Croquemort (Crq) are specifically expressed in embryonic macrophages and required for their phagocytic function. However, how this function is established during development remains unclear. Here we demonstrate that the key regulator of Drosophila embryonic hemocyte differentiation, the transcription factor Serpent (Srp), plays a central role in establishing macrophage phagocytic competence. Srp, a homolog of the mammalian GATA factors, is required and sufficient for the specific expression of SIMU, Drpr and Crq receptors in embryonic macrophages. Moreover, we show that each of these receptors can significantly rescue phagocytosis defects of macrophages in srp mutants, including their distribution in the embryo and engulfment of apoptotic cells. This reveals that the proficiency of macrophages to remove apoptotic cells relies on the expression of SIMU, Crq and/or Drpr. However, Glial Cells Missing (GCM) acting downstream of Srp in the differentiation of hemocytes, is dispensable for their phagocytic function during embryogenesis. Taken together, our study discloses the molecular mechanism underlying the development of macrophages as skilled phagocytes of apoptotic cells.

PubMed ID
PubMed Central ID
PMC5852079 (PMC) (EuropePMC)
Related Publication(s)
Note

Commentary: Drosophila GATA Factor Serpent Establishes Phagocytic Ability of Embryonic Macrophages.
Valanne et al., 2018, Front. Immunol. 9: 1582 [FBrf0239502]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Front. Immunol.
    Title
    Frontiers in immunology
    ISBN/ISSN
    1664-3224
    Data From Reference