Open Close
Reference
Citation
Boukhatmi, H., Bray, S. (2018). A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms.  eLife 7(): e35954.
FlyBase ID
FBrf0238778
Publication Type
Research paper
Abstract

Adult stem cells are important for tissue maintenance and repair. One key question is how such cells are specified and then protected from differentiation for a prolonged period. Investigating the maintenance of Drosophila muscle progenitors (MPs) we demonstrate that it involves a switch in zfh1/ZEB1 RNA-isoforms. Differentiation into functional muscles is accompanied by expression of miR-8/miR-200, which targets the major zfh1-long RNA isoform and decreases Zfh1 protein. Through activity of the Notch pathway, a subset of MPs produce an alternate zfh1-short isoform, which lacks the miR-8 seed site. Zfh1 protein is thus maintained in these cells, enabling them to escape differentiation and persist as MPs in the adult. There, like mammalian satellite cells, they contribute to muscle homeostasis. Such preferential regulation of a specific RNA isoform, with differential sensitivity to miRs, is a powerful mechanism for maintaining a population of poised progenitors and may be of widespread significance.

PubMed ID
PubMed Central ID
PMC5919756 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for the zfh1[DeltaEnh3] deletion.
Boukhatmi and Bray, 2019.1.27, Location data for the zfh1[DeltaEnh3] deletion. [FBrf0241308]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference