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Citation
Krueger, D., Tardivo, P., Nguyen, C., De Renzis, S. (2018). Downregulation of basal myosin-II is required for cell shape changes and tissue invagination.  EMBO J. 37(23): e100170.
FlyBase ID
FBrf0240722
Publication Type
Research paper
Abstract

Tissue invagination drives embryo remodeling and assembly of internal organs during animal development. While the role of actomyosin-mediated apical constriction in initiating inward folding is well established, computational models suggest relaxation of the basal surface as an additional requirement. However, the lack of genetic mutations interfering specifically with basal relaxation has made it difficult to test its requirement during invagination so far. Here we use optogenetics to quantitatively control myosin-II levels at the basal surface of invaginating cells during Drosophila gastrulation. We show that while basal myosin-II is lost progressively during ventral furrow formation, optogenetics allows the maintenance of pre-invagination levels over time. Quantitative imaging demonstrates that optogenetic activation prior to tissue bending slows down cell elongation and blocks invagination. Activation after cell elongation and tissue bending has initiated inhibits cell shortening and folding of the furrow into a tube-like structure. Collectively, these data demonstrate the requirement of myosin-II polarization and basal relaxation throughout the entire invagination process.

PubMed ID
PubMed Central ID
PMC6276876 (PMC) (EuropePMC)
Related Publication(s)
Note

Optogenetic control of morphogenesis goes 3D.
Thompson, 2018, EMBO J. 37(23): e100961 [FBrf0243609]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference