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Lee, S.K., Xue, Y., Shen, W., Zhang, Y., Joo, Y., Ahmad, M., Chinen, M., Ding, Y., Ku, W.L., De, S., Lehrmann, E., Becker, K.G., Lei, E.P., Zhao, K., Zou, S., Sharov, A., Wang, W. (2018). Topoisomerase 3β interacts with RNAi machinery to promote heterochromatin formation and transcriptional silencing in Drosophila.  Nat. Commun. 9(1): 4946.
FlyBase ID
FBrf0240783
Publication Type
Research paper
Abstract

Topoisomerases solve topological problems during DNA metabolism, but whether they participate in RNA metabolism remains unclear. Top3β represents a family of topoisomerases carrying activities for both DNA and RNA. Here we show that in Drosophila, Top3β interacts biochemically and genetically with the RNAi-induced silencing complex (RISC) containing AGO2, p68 RNA helicase, and FMRP. Top3β and RISC mutants are similarly defective in heterochromatin formation and transcriptional silencing by position-effect variegation assay. Moreover, both Top3β and AGO2 mutants exhibit reduced levels of heterochromatin protein HP1 in heterochromatin. Furthermore, expression of several genes and transposable elements in heterochromatin is increased in the Top3β mutant. Notably, Top3β mutants defective in either RNA binding or catalytic activity are deficient in promoting HP1 recruitment and silencing of transposable elements. Our data suggest that Top3β may act as an RNA topoisomerase in siRNA-guided heterochromatin formation and transcriptional silencing.

PubMed ID
PubMed Central ID
PMC6251927 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for AGO2 deletions.
Lei and Chinen, 2020.6.24, Location data for AGO2 deletions. [FBrf0245995]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference