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Finegan, T.M., Na, D., Cammarota, C., Skeeters, A.V., NĂ¡dasi, T.J., Dawney, N.S., Fletcher, A.G., Oakes, P.W., Bergstralh, D.T. (2019). Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium.  EMBO J. 38(3): e100072.
FlyBase ID
FBrf0241334
Publication Type
Research paper
Abstract

We investigated the cell behaviors that drive morphogenesis of the Drosophila follicular epithelium during expansion and elongation of early-stage egg chambers. We found that cell division is not required for elongation of the early follicular epithelium, but drives the tissue toward optimal geometric packing. We examined the orientation of cell divisions with respect to the planar tissue axis and found a bias toward the primary direction of tissue expansion. However, interphase cell shapes demonstrate the opposite bias. Hertwig's rule, which holds that cell elongation determines division orientation, is therefore broken in this tissue. This observation cannot be explained by the anisotropic activity of the conserved Pins/Mud spindle-orienting machinery, which controls division orientation in the apical-basal axis and planar division orientation in other epithelial tissues. Rather, cortical tension at the apical surface translates into planar division orientation in a manner dependent on Canoe/Afadin, which links actomyosin to adherens junctions. These findings demonstrate that division orientation in different axes-apical-basal and planar-is controlled by distinct, independent mechanisms in a proliferating epithelium.

PubMed ID
PubMed Central ID
PMC6356066 (PMC) (EuropePMC)
Related Publication(s)
Note

Getting into shape: tissue tension drives oriented cell divisions during organogenesis.
Manning and Peifer, 2019, EMBO J. 38(3): e101246 [FBrf0244369]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Genes (12)