Open Close
Li, J.S.S., Sean Millard, S. (2019). Deterministic splicing of Dscam2 is regulated by Muscleblind.  Sci. Adv. 5(1): eaav1678.
FlyBase ID
Publication Type
Research paper

Alternative splicing increases the proteome diversity crucial for establishing the complex circuitry between trillions of neurons. To provide individual cells with different repertoires of protein isoforms, however, this process must be regulated. Previously, we found that the mutually exclusive alternative splicing of Drosophila Dscam2 produces two isoforms (A and B) with unique binding properties. This splicing event is cell type specific, and the transmembrane proteins that it generates are crucial for the development of axons, dendrites, and synapses. Here, we show that Muscleblind (Mbl) controls Dscam2 alternative splicing. Mbl represses isoform A and promotes the selection of isoform B. Mbl mutants exhibit phenotypes also observed in flies engineered to express a single Dscam2 isoform. Consistent with this, mbl expression is cell type specific and correlates with the splicing of isoform B. Our study demonstrates how the regulated expression of a splicing factor is sufficient to provide neurons with unique protein isoforms crucial for development.

PubMed ID
PubMed Central ID
PMC6357765 (PMC) (EuropePMC)
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Sci. Adv.
    Science advances
    Data From Reference
    Aberrations (2)
    Alleles (35)
    Genes (9)
    Polypeptides (1)
    Insertions (12)
    Transgenic Constructs (14)
    Transcripts (5)