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Citation
Li, J.S.S., Sean Millard, S. (2019). Deterministic splicing of Dscam2 is regulated by Muscleblind.  Sci. Adv. 5(1): eaav1678.
FlyBase ID
FBrf0241446
Publication Type
Research paper
Abstract

Alternative splicing increases the proteome diversity crucial for establishing the complex circuitry between trillions of neurons. To provide individual cells with different repertoires of protein isoforms, however, this process must be regulated. Previously, we found that the mutually exclusive alternative splicing of Drosophila Dscam2 produces two isoforms (A and B) with unique binding properties. This splicing event is cell type specific, and the transmembrane proteins that it generates are crucial for the development of axons, dendrites, and synapses. Here, we show that Muscleblind (Mbl) controls Dscam2 alternative splicing. Mbl represses isoform A and promotes the selection of isoform B. Mbl mutants exhibit phenotypes also observed in flies engineered to express a single Dscam2 isoform. Consistent with this, mbl expression is cell type specific and correlates with the splicing of isoform B. Our study demonstrates how the regulated expression of a splicing factor is sufficient to provide neurons with unique protein isoforms crucial for development.

PubMed ID
PubMed Central ID
PMC6357765 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Sci. Adv.
    Title
    Science advances
    ISBN/ISSN
    2375-2548
    Data From Reference
    Aberrations (2)
    Alleles (35)
    Genes (9)
    Polypeptides (1)
    Insertions (12)
    Transgenic Constructs (14)
    Transcripts (5)