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Tsarouhas, V., Liu, D., Tsikala, G., Fedoseienko, A., Zinn, K., Matsuda, R., Billadeau, D.D., Samakovlis, C. (2019). WASH phosphorylation balances endosomal versus cortical actin network integrities during epithelial morphogenesis.  Nat. Commun. 10(1): 2193.
FlyBase ID
FBrf0242366
Publication Type
Research paper
Abstract

Filamentous actin (F-actin) networks facilitate key processes like cell shape control, division, polarization and motility. The dynamic coordination of F-actin networks and its impact on cellular activities are poorly understood. We report an antagonistic relationship between endosomal F-actin assembly and cortical actin bundle integrity during Drosophila airway maturation. Double mutants lacking receptor tyrosine phosphatases (PTP) Ptp10D and Ptp4E, clear luminal proteins and disassemble apical actin bundles prematurely. These defects are counterbalanced by reduction of endosomal trafficking and by mutations affecting the tyrosine kinase Btk29A, and the actin nucleation factor WASH. Btk29A forms protein complexes with Ptp10D and WASH, and Btk29A phosphorylates WASH. This phosphorylation activates endosomal WASH function in flies and mice. In contrast, a phospho-mimetic WASH variant induces endosomal actin accumulation, premature luminal endocytosis and cortical F-actin disassembly. We conclude that PTPs and Btk29A regulate WASH activity to balance the endosomal and cortical F-actin networks during epithelial tube maturation.

PubMed ID
PubMed Central ID
PMC6522504 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

stitcher CA fly line.
Tsarouhas, 2019.6.28, stitcher CA fly line. [FBrf0242878]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference