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Gärtner, S.M.K., Hundertmark, T., Nolte, H., Theofel, I., Eren-Ghiani, Z., Tetzner, C., Duchow, T.B., Rathke, C., Krüger, M., Renkawitz-Pohl, R. (2019). Stage-specific testes proteomics of Drosophila melanogaster identifies essential proteins for male fertility.  Europ. J. Cell Biol. 98(2-4): 103--115.
FlyBase ID
FBrf0242499
Publication Type
Research paper
Abstract

Spermiogenesis in Drosophila melanogaster is a highly conserved process and essential for male fertility. In this haploid phase of spermatogenesis, motile sperm are assembled from round cells, and flagella and needle-shaped nuclei with highly compacted genomes are formed. As transcription takes place mainly in spermatocytes and transcripts relevant for post-meiotic sperm development are translationally repressed for days, we comparatively analysed the proteome of larval testes (only germ cell stages before meiotic divisions), testes of 1-2-day-old pupae (germ cell stages before meiotic divisions, meiotic and early spermatid stages) and adult flies (germ cell stages before meiotic divisions, meiotic and early spermatid stages, late spermatids and sperm). We identified 6,171 proteins; 61 proteins were detected solely in one stage and are thus enriched, namely 34 in larval testes, 77 in pupal testes and 214 in adult testes. To substantiate our mass spectrometric data, we analysed the stage-specific synthesis and importance for male fertility of a number of uncharacterized proteins. For example, Mst84B (gene CG1988), a very basic cysteine- and lysine-rich nuclear protein and was present in the transition phase from a histone-based to a protamine-based chromatin structure. CG6332 encodes d-Theg, which is related to the mouse tHEG and human THEG proteins. Mutants of d-Theg were sterile due to the lack of sperm in the seminal vesicles. Our catalogue of proteins of the different stages of testis development in D. melanogaster will pave the road for future analyses of spermatogenesis.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Europ. J. Cell Biol.
    Title
    European Journal of Cell Biology
    Publication Year
    1979-
    ISBN/ISSN
    0171-9335
    Data From Reference
    Aberrations (3)
    Alleles (6)
    Genes (24)
    Natural transposons (2)
    Insertions (5)
    Experimental Tools (1)
    Transgenic Constructs (4)