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Masuzzo, A., Manière, G., Viallat-Lieutaud, A., Avazeri, É., Zugasti, O., Grosjean, Y., Kurz, C.L., Royet, J. (2019). Peptidoglycan-dependent NF-κB activation in a small subset of brain octopaminergic neurons controls female oviposition.  eLife 8(): e50559.
FlyBase ID
FBrf0243879
Publication Type
Research paper
Abstract

When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-κB pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-κB pathway component. We functionally demonstrated that NF-κB pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-κB cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.

PubMed ID
PubMed Central ID
PMC6819134 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (27)
    Genes (17)
    Natural transposons (1)
    Insertions (4)
    Experimental Tools (4)
    Transgenic Constructs (26)