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Citation
Gao, Y., Zhang, X., Xiao, L., Zhai, C., Yi, T., Wang, G., Wang, E., Ji, X., Hu, L., Shen, G., Wu, S. (2019). Usp10 Modulates the Hippo Pathway by Deubiquitinating and Stabilizing the Transcriptional Coactivator Yorkie.  Int. J. Mol. Sci. 20(23): E6013.
FlyBase ID
FBrf0244243
Publication Type
Research paper
Abstract

The Hippo signaling pathway is an evolutionarily conserved regulator that plays important roles in organ size control, homeostasis, and tumorigenesis. As the key effector of the Hippo pathway, Yorkie (Yki) binds to transcription factor Scalloped (Sd) and promotes the expression of target genes, leading to cell proliferation and inhibition of apoptosis. Thus, it is of great significance to understand the regulatory mechanism for Yki protein turnover. Here, we provide evidence that the deubiquitinating enzyme ubiquitin-specific protease 10 (Usp10) binds Yki to counteract Yki ubiquitination and stabilize Yki protein in Drosophila S2 cells. The results in Drosophila wing discs indicate that silence of Usp10 decreases the transcription of target genes of the Hippo pathway by reducing Yki protein. In vivo functional analysis ulteriorly showed that Usp10 upregulates the Yki activity in Drosophila eyes. These findings uncover Usp10 as a novel Hippo pathway modulator and provide a new insight into the regulation of Yki protein stability and activity.

PubMed ID
PubMed Central ID
PMC6928647 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Int. J. Mol. Sci.
    Title
    International journal of molecular sciences
    ISBN/ISSN
    1422-0067
    Data From Reference
    Alleles (7)
    Genes (6)
    Physical Interactions (2)
    Cell Lines (1)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (7)