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Zhang, L., Xu, W., Gao, X., Li, W., Qi, S., Guo, D., Ajayi, O.E., Ding, S.W., Wu, Q. (2020). lncRNA Sensing of a Viral Suppressor of RNAi Activates Non-canonical Innate Immune Signaling in Drosophila.  Cell Host Microbe 27(1): 115--128.e8.
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Research paper

Antiviral immunity in insects is mediated by the RNA interference (RNAi) pathway. Viruses evade antiviral RNAi by expressing virulence factors known as viral suppressors of RNAi (VSR). Here, we report the identification of VINR, a Drosophila VSR-interacting long non-coding (lnc) RNA that activates non-canonical innate immune signaling upon detection of the dsRNA-binding VSR of Drosophila C virus (DCV). VINR is required for the induction of antimicrobial peptide (AMP) genes but dispensable for antiviral RNAi. VINR functions by preventing the ubiquitin proteasome-dependent degradation of Cactin, a coiled-coil and arginine-serine-rich domain-containing protein that regulates a non-cannonical antimicrobial pathway for AMP induction. CRISPR-Cas9 knockout of VINR in Drosophila cells enhances DCV replication independently of antiviral RNAi, and VINR-knockout adult flies exhibit enhanced disease susceptibility to DCV and bacteria. Our findings reveal a counter counter-defense strategy activated by a lncRNA in response to the viral suppression of the primary antiviral RNAi immunity.

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Personal communication to FlyBase

Location data for lncRNA:Vinr[KO].
Zhang and Wu, 2020.3.11, Location data for lncRNA:Vinr[KO]. [FBrf0245090]


Countering Counter-Defense to Antiviral RNAi.
Betting and Van Rij, 2020, Trends Microbiol. 28(8): 600--602 [FBrf0246234]

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    Cell Host Microbe
    Cell Host & Microbe
    Publication Year
    1931-3128 1934-6069
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    Alleles (1)
    Genes (30)
    Physical Interactions (6)
    Cell Lines (1)