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Sidor, C., Stevens, T.J., Jin, L., Boulanger, J., Röper, K. (2020). Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time.  Dev. Cell 52(3): 364--378.e7.
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Research paper

The myosin II activator Rho-kinase (Rok) is planar polarized at the tissue boundary of the Drosophila embryonic salivary gland placode through a negative regulation by the apical polarity protein Crumbs that is anisotropically localized at the boundary. However, in inner cells of the placode, both Crumbs and Rok are isotropically enriched at junctions. We propose that modulation of Rok membrane residence time by Crumbs' downstream effectors can reconcile both behaviors. Using FRAP combined with in silico simulations, we find that the lower membrane dissociation rate (koff) of Rok at the tissue boundary with low Crumbs explains this boundary-specific effect. The S/T kinase Pak1, recruited by Crumbs and Cdc42, negatively affects Rok membrane association in vivo and in vitro can phosphorylate Rok near the pleckstrin homology (PH) domain that mediates membrane association. These data reveal an important mechanism of the modulation of Rok membrane residence time via affecting the koff that may be widely employed during tissue morphogenesis.

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PubMed Central ID
PMC7008249 (PMC) (EuropePMC)
Related Publication(s)

Good Fences Make Good Neighbors: Crumbs Regulates Rho-Kinase Dynamics to Assemble a Tissue Boundary.
Yu-Kemp and Peifer, 2020, Dev. Cell 52(3): 255--256 [FBrf0246279]

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    Parent Publication
    Publication Type
    Dev. Cell
    Developmental Cell
    Publication Year
    1534-5807 1878-1551
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