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Citation
Qin, Q.M., Pei, J., Gomez, G., Rice-Ficht, A., Ficht, T.A., de Figueiredo, P. (2020). A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors.  Front. Cell. Infect. Microbiol. 10(): 240.
FlyBase ID
FBrf0245912
Publication Type
Research paper
Abstract

Acinetobacter baumannii is an important causative agent of nosocomial infections worldwide. The pathogen also readily acquires resistance to antibiotics, and pan-resistant strains have been reported. A. baumannii is widely regarded as an extracellular bacterial pathogen. However, accumulating evidence demonstrates that the pathogen can invade, survive or persist in infected mammalian cells. Unfortunately, the molecular mechanisms controlling these processes remain poorly understood. Here, we show that Drosophila S2 cells provide several attractive advantages as a model system for investigating the intracellular lifestyle of the pathogen, including susceptibility to bacterial intracellular replication and limited infection-induced host cell death. We also show that the Drosophila system can be used to rapidly identify host factors, including MAP kinase proteins, which confer susceptibility to intracellular parasitism. Finally, analysis of the Drosophila system suggested that host proteins that regulate organelle biogenesis and membrane trafficking contribute to regulating the intracellular lifestyle of the pathogen. Taken together, these findings establish a novel model system for elucidating interactions between A. baumannii and host cells, define new factors that regulate bacterial invasion or intracellular persistence, and identify subcellular compartments in host cells that interact with the pathogen.

PubMed ID
PubMed Central ID
PMC7264411 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Front. Cell. Infect. Microbiol.
    Title
    Frontiers in cellular and infection microbiology
    ISBN/ISSN
    2235-2988
    Data From Reference
    Genes (17)
    Cell Lines (1)