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Citation
Matsubayashi, Y., Sánchez-Sánchez, B.J., Marcotti, S., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M.D., Vizcay-Barrena, G., Fleck, R.A., Stramer, B.M. (2020). Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis.  Dev. Cell 54(1): 33--42.e9.
FlyBase ID
FBrf0246169
Publication Type
Research paper
Abstract

The extracellular matrix (ECM) is a polymer network hypothesized to form a stable cellular scaffold. While the ECM can undergo acute remodeling during embryogenesis, it is experimentally difficult to determine whether basal turnover is also important. Most studies of homeostatic turnover assume an initial steady-state balance of production and degradation and measure half-life by quantifying the rate of decay after experimental intervention (e.g., pulse labeling). Here, we present an intervention-free approach to mathematically model basal ECM turnover during embryogenesis by exploiting our ability to live image de novo ECM development in Drosophila to quantify production from initiation to homeostasis. This reveals rapid turnover (half-life ∼7-10 h), which we confirmed by in vivo pulse-chase experiments. Moreover, ECM turnover is partially dependent on proteolysis and network interactions, and slowing turnover affects tissue morphogenesis. These data demonstrate that embryonic ECM undergoes constant replacement, which is likely necessary to maintain network plasticity to accommodate growth and morphogenesis.

PubMed ID
PubMed Central ID
PMC7332994 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference