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Citation
Miao, G., Godt, D., Montell, D.J. (2020). Integration of Migratory Cells into a New Site In Vivo Requires Channel-Independent Functions of Innexins on Microtubules.  Dev. Cell 54(4): 501--515.e9.
FlyBase ID
FBrf0246573
Publication Type
Research paper
Abstract

During embryonic development and cancer metastasis, migratory cells must establish stable connections with new partners at their destinations. Here, we establish the Drosophila border cells as a model for this multistep process. During oogenesis, border cells delaminate from the follicular epithelium and migrate. When they reach their target, the oocyte, they undergo a stereotypical series of steps to adhere to it, then connect with another migrating epithelium. We identify gap-junction-forming innexin proteins as critical. Surprisingly, the channel function is dispensable. Instead, Innexins 2 and 3 function within the border cells, and Innexin 4 functions within the germline, to regulate microtubules. The microtubule-dependent border cell-oocyte interaction is essential to brace the cells against external morphogenetic forces. Thus, we establish an experimental model and use genetic, thermogenetic, and live-imaging approaches to uncover the contributions of Innexins and microtubules to a cell-biological process important in development and cancer.

PubMed ID
PubMed Central ID
PMC7484434 (PMC) (EuropePMC)
Related Publication(s)
Note

Building Relationships: A Role for Innexins in Tissue Formation.
Labbaf and Raz, 2020, Dev. Cell 54(4): 428--430 [FBrf0246600]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference
    Alleles (41)
    Genes (12)
    Natural transposons (1)
    Insertions (6)
    Experimental Tools (2)
    Transgenic Constructs (36)