Open Close
Reference
Citation
Trivedi, D., Cm, V., Bisht, K., Janardan, V., Pandit, A., Basak, B., H, S., Ramesh, N., Raghu, P. (2020). A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling.  eLife 9(): e55793.
FlyBase ID
FBrf0247664
Publication Type
Research paper
Abstract

Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms. Biochemical analyses and studies in cultured cells have identified a large number of proteins that can mediate PI signaling. However, the role of such proteins in regulating cellular processes in vivo and development in metazoans remains to be understood. Here, we describe a set of CRISPR-based genome engineering tools that allow the manipulation of each of these proteins with spatial and temporal control during metazoan development. We demonstrate the use of these reagents to deplete a set of 103 proteins individually in the Drosophila eye and identify several new molecules that control eye development. Our work demonstrates the power of this resource in uncovering the molecular basis of tissue homeostasis during normal development and in human disease biology.

PubMed ID
PubMed Central ID
PMC7771963 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (119)
    Genes (106)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (107)
    Experimental Tools (3)
    Transgenic Constructs (113)