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Citation
Xie, Q., Brbic, M., Horns, F., Kolluru, S.S., Jones, R.C., Li, J., Reddy, A.R., Xie, A., Kohani, S., Li, Z., McLaughlin, C.N., Li, T., Xu, C., Vacek, D., Luginbuhl, D.J., Leskovec, J., Quake, S.R., Luo, L., Li, H. (2021). Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons.  eLife 10(): e63450.
FlyBase ID
FBrf0247975
Publication Type
Research paper
Abstract

Neurons undergo substantial morphological and functional changes during development to form precise synaptic connections and acquire specific physiological properties. What are the underlying transcriptomic bases? Here, we obtained the single-cell transcriptomes of Drosophila olfactory projection neurons (PNs) at four developmental stages. We decoded the identity of 21 transcriptomic clusters corresponding to 20 PN types and developed methods to match transcriptomic clusters representing the same PN type across development. We discovered that PN transcriptomes reflect unique biological processes unfolding at each stage-neurite growth and pruning during metamorphosis at an early pupal stage; peaked transcriptomic diversity during olfactory circuit assembly at mid-pupal stages; and neuronal signaling in adults. At early developmental stages, PN types with adjacent birth order share similar transcriptomes. Together, our work reveals principles of cellular diversity during brain development and provides a resource for future studies of neural development in PNs and other neuronal types.

PubMed ID
PubMed Central ID
PMC7870145 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (27)
    Genes (154)
    Insertions (13)
    Transgenic Constructs (14)