Open Close
Reference
Citation
Zhang, P., Katzaroff, A.J., Buttitta, L.A., Ma, Y., Jiang, H., Nickerson, D.W., Øvrebø, J.I., Edgar, B.A. (2021). The Krüppel-like factor Cabut has cell cycle regulatory properties similar to E2F1.  Proc. Natl. Acad. Sci. U.S.A. 118(7): e2015675118.
FlyBase ID
FBrf0248123
Publication Type
Research paper
Abstract

Using a gain-of-function screen in Drosophila, we identified the Krüppel-like factor Cabut (Cbt) as a positive regulator of cell cycle gene expression and cell proliferation. Enforced cbt expression is sufficient to induce an extra cell division in the differentiating fly wing or eye, and also promotes intestinal stem cell divisions in the adult gut. Although inappropriate cell proliferation also results from forced expression of the E2f1 transcription factor or its target, Cyclin E, Cbt does not increase E2F1 or Cyclin E activity. Instead, Cbt regulates a large set of E2F1 target genes independently of E2F1, and our data suggest that Cbt acts via distinct binding sites in target gene promoters. Although Cbt was not required for cell proliferation during wing or eye development, Cbt is required for normal intestinal stem cell divisions in the midgut, which expresses E2F1 at relatively low levels. The E2F1-like functions of Cbt identify a distinct mechanism for cell cycle regulation that may be important in certain normal cell cycles, or in cells that cycle inappropriately, such as cancer cells.

PubMed ID
PubMed Central ID
PMC7896318 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Aberrations (3)
    Alleles (45)
    Genes (28)
    Natural transposons (1)
    Insertions (15)
    Experimental Tools (3)
    Transgenic Constructs (21)