FB2022_02, released March 29, 2022

Sequence Feature: Dmel\DRSC11405

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General Information
Symbol
Dmel\DRSC11405
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000011025
Collection
Associated gene
Genomic Location
Chromosome (arm)
3L
Sequence location

3L:16,590,365..16,590,752 [-]

Genomic Maps
Sequence Data
Length
388
Comments

One copy of repeat shown

(Booker, 2006.5)

Sequence

Sequence Downloader
GGTGGCACTTGCACATAGTAGTTGATGATCTTTGGCGGAGGATTATACCGATGCGGGCTCCGTTGACGGCGTCTTCGTTC
ACTGCTGCGACTTCGGCTATGTTGACGGCGTTTTCGTTCACTGCTGCGACTTCGGTTGCGATTGCGGCTCCTAGAGCGCT
GGCGATGCCTTCTGTGCCTGCTCTCTCTGATGGACGACTGTGATCTGGATCTGGAGCGAGTGCGTCTGGTGGATTGGTGC
GCTCTTTGGCGCAATCTTCTCAACCGATCCTGTTCTCGAACCTCGTCTGCAAAGTACGGAATCTTGTGCTCCTTCTTTCT
GCTGTCCCTCTCGCTTGATCGCCCATGGTATTCTCTTTCTCGCCGGGATCGAGACCTTGAGCCACGGG
Associated Information
Gene(s) (targeted or local)
Allele(s)
    Transcripts(s)
      Polypeptide(s)
        Construct
          Experimental Data
          Progenitors
          PCR template

          genomic DNA

          (Booker, 2006.5)
          Primer 1 
          CCCGTGGCTCAAGGTCT
          (Booker, 2006.5)
          Primer 2 
          GGTGGCACTTGCACATAGT
          (Booker, 2006.5)
          Comments
          Collection Information
          Collection: DRSC_dsRNA
          Symbol
          DRSC_dsRNA
          Title
          DRSC collection of dsRNA for RNAi in cell culture.
          Source and Progenitors
          Species of derivation
          D. melanogaster
          Strain of derivation
          Vector of progenitor construct
          Description

          Comprehensive collection of double-strand RNA fragments (1-2 dsRNAs per annotated gene), used for genome-wide RNAi-knockdown assays in cell culture.

          Additional data
          Sample preparation
          Collection preparation

          The DRSC RNAi screening library was constructed from genomic DNA amplified using Oregon-R strain. In initial collection, most amplicons were designed to FlyBase rel2 at R.Paro's lab (ZMBH Heidelberg). Primers were selected to target the open reading frames (ORF) of known and predicted genes. Regularly updated to accommodate newly identified genes and new gene models, and to replace amplicons with high potential for off-target effects.

          (Flockhart et al., 2006)
          Mode of assay
          Data analysis
          Cell lines
          Observed in
          Not observed in
          Comments
          Stocks (0)
          External Crossreferences and Linkouts ( 1 )
          Crossreferences
          DRSC - Results frm RNAi screens
          Synonyms and Secondary IDs (2)
          Reported As
          Symbol Synonym
          DRSC11405
          (M. Booker, 2008.6.2)
          HDC10531
          (Booker, 2006.5)
          Secondary FlyBase IDs
          • FBri0010932
          References (2)