Schneider
S2R+ cells stably expressing Actin-Gal4 ( source:Prof . Satyajit Mayor lab, NCBS, Bangalore-India).
S2R+ cells stably expressing LiveDrop-GFP or f S2 cells stably expressing LiveDrop-RFP were used.
Stable cell line generated: A stable S2 cell line deleted for the Pex1 gene was created.
S2R + cells were obtained from the laboratory of Sven Bogdan.
S2R+ (FEx 2.5%), S2R+ cells supplemented with adult fly extract used.
S2R+ cells were obtained from the Carthew lab.
S2R+ cells were obtained from the laboratory of N. Perrimon.
Source of S2R+ cells: DGRC.
Source of S2R+ cells: E. Chen (UT Southwestern).
Stable cell line generated: S2R+ cells were stably transfected with copper-inducible Myc-tagged Clk1alpha.
Stable cell line generated:An Act-PE2 (prime editor 2) cell line was generated for use in prime editing. PE2 is expressed under the Act5C promoter.
S2R+ cells in which the fdl gene is deleted were obtained from the laboratory of D. Jarvis.
Stable cell line generated:A stable S2R+ cell line was generated lacking endogenous Stat92E by CRISPR/Cas9-mediated mutatgenesis.
Both S2R+ and S2R+Wb (Wolbachia) cells were used.
S2R+ cells were obtained from the laboratory of N. Yanagawa.
Stable cell line generated: Stable cell lines were generated to express constructs containing destabilizing domains (genetic tags that conditionally control the level of abundance of proteins of interest with specific stabilizing small-molecule drugs).
Stable cell line generated: a stable S2R+ cell line expressing AkhR was generated.
Expression of general hemocyte markers observed.
Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16287).
SR2+ is an isolate of S2 cells that was found in the Miyake laboratory freezer. It was contributed to the Miyake lab by Imogene Schneider and is likely to be very similar to the original S2 line.
Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive.