FB2025_01 , released February 20, 2025
Cell Line: S2R+-MT::Cas9
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General Information
Symbol
S2R+-MT::Cas9
Species
D. melanogaster
Feature type
cultured cell-line
FlyBase ID
FBtc0000268
Source
Tissue Source
embryo
(Drosophila Genomics Resource Center, 2018.7.9)
Developmental Stage
late embryonic stage
(Drosophila Genomics Resource Center, 2018.7.9)
Lab of Origin

Perrimon

(Drosophila Genomics Resource Center, 2018.7.9)
Characterization
Sex
Karyotype
Associated Genes (0)
    Integrated Constructs (0)
      Comments

      New stable cell line derived from this cell line: vsg KO lines were created in S2R+- MT::Cas9 cells.

      (Xu et al., 2022)

      New stable cell line derived from this cell line: sloth1 and sloth2 KO cell lines were created.

      (Bosch et al., 2022)

      New stable cell line derived from this cell line: The following stable knockout S2 cell lines were created: Arip4KO (CG4049), ldsKO, domKO, RecQ5KO, brmKO, and Mi-2KO.

      (Deger et al., 2022)

      This cell line was developed to support CRISPR pooled-format screening in Drosophila cells. The starting cell line used to generate S2R+-MT::Cas9, S2R+-NPT005, was created using MiMIC technology and is described in Neumuller et al. 2012 (FBrf0217811). N-terminally flag-tagged Cas9 driven by a metallothionein promotor was transfected into S2R+-NPT005 and stable transfected cells were derived to generate S2R+-MT::Cas9. Attributes of this cell line include: S2R+ derivative; expresses mCherry ::Clic fusion protein; attP sites present; pMT-Flag-Cas9.

      (Drosophila Genomics Resource Center, 2018.7.9)
      Parental Lines (1)
      Transformed from:
      Descendant Lines (26)
      External Stocks and Resources ( 3 )
      Crossreferences
      Cellosaurus - Cellosaurus is a knowledge resource on cell lines that attempts to describe all cell lines used in biomedical research.
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      RRID - Research Resource Identification Portal
      Synonyms and Secondary IDs (11)
      References (15)