Stable cell line generated: A stable S2 cell line expressing Yellow-y-SBP.
source - CCTCC (may have meant ATCC).
Cell line reported as S2-GAL4.
S2 cells stably expressing hh-N were obtained from the laboratory of J.Jiang.
S2 cells were obtained from the laboratory of Dr. H. Siomi.
S2 cells were obtained from the laboratory of Dr. Kumiko Ui-Te.
S2 cells were obtained from the laboratory of Dr. N. E. Vorobyeva.
S2 cells were obtained from the laboratory of Dr. N. Vorobyeva.
S2 cells were obtained from the laboratory of Erika Geisbrecht.
S2 cells, subclone L2-4, were obtained from the laboratory of P. Huen.
S2[Cas9] cells were obtained from the laboratory of Klaus Forstemann.
Stable cell line generated: An S2 cell line stably expressing porcine PoIFN-&gr;8 was generated.
Stable cell line generated: Drosophila codon optimized or deoptimized firefly luciferase expressed under the control of various promoters.
Stable cell line generated: S2 cell lines stably expressing wild type or mutant Rdl GABA receptor genes were constructed.
Stable cell line generated: S2 cells were stably transfected with pMT-ET-V5 (to overexpress V5-tagged et protein) with or without pMT-upd1-myc (to activate the JAK/STAT pathway).
Stable cell line generated:Stable S2 Mettl3-KO cell lines were generated by CRISPR/Cas9-mediated mutatgenesis.
A scra- FBto0000118:mCherry S2 cell line was used.
S2 cells were obtained from Expression Systems, 94-005F.
S2 cells were obtained from the laboratory of Don Rio.
S2 cells were obtained from the laboratory of P. Zamore.
Stable cell line generated: A stable S2 cell line expressing 3XHA-tagged Nipped-A was created.
Stable cell line generated: A stable S2 cell line was created that expresses SCAT3m a FRET-based caspase activity indicator.
Stable cell line generated: CRISPR genome-edited cell lines were created carrying Sec71[M717L] or Sec71[F713Y]. These S2 cells were stably transfected with pMT-GalT-EGFP-T2A-tdTomato-Rab6 or pMT- GalT::EGFP-T2A-tdTomato::Rab11 .
Stable cell line generated: CRISPR/Cas9 used to tag endogenous Spt6 with GFP; stably transfected versions of the resulting Spt6-GFP cell line were generated with a small molecule inducible version of the geGradeFP system, a tamoxifen inducible HA-cid and/or a version of cid capable of recombination induced tag exchange (RITE).
Stable cell line generated: S2 cells (subclone L2-4) stably expressing DHX9-GFP were establishedS2 cells were obtained from the laboratory of Philipp Zamore. The S2 subclone L2-4 was obtained from the laboratory of Patrick Heun.
Stable cell line generated: S2 cells expressing FLAG-tagged deletion forms of BigH1.
Stable cell line generated: Stable S2 cell line expressing FLAG-tagged human EZHIP under the control of the MT promoter was created.
Stable cell line generated: Two stable S2 cell lines were created that are knockouts for lncRNA:Vinr.
Stable cell lines generated: Clonal S2 cells harboring mutations in CG34401 were created.
Cells obtained from RIKEN Bioresource Center.
L2-4 (S2 subclone, provided by P. Heun).
S2 cells expressing GFP-Utrophin-CH/GFP-Tubby and mCherry-tubulin and S2 cells expressing anilllin-mCherry and GFP-Spaghetti-squash from the laboratory of Gilles Hickson were used.
S2 cells were obtained from the laboratory of Alain Debec.
S2 cells were obtained from the laboratory of Erjun Ling.
S2 cells were obtained from the laboratory of F. Feiguin.
S2 cells were obtained from the laboratory of J.H. Kim.
S2 cells were obtained from the laboratory of Mikiko Siomi (University of Tokyo).
S2 cells were obtained from the laboratory of N. Silverman.
S2 cells were obtained from the laboratory of Patrick Emery.
S2 cells were obtained from the laboratory of Sebastien Carreno.
Stable S2 cell lines expressing Ada2b isoform B were used.
Stable cell line generated: An inducible CRISPR/Cas9 system was developed that generates double strand breaks in the 11- to 12-base-pair (bp) dodeca satellite tandem repeats located in the chromosome 3 pericentromere.
Stable cell line generated: S2 cells stably expressing a pcs construct ( myc::APEX2::Pcs ) were generated. S2 cells were obtained from the laboratory of Dr. Gota Goshima at Nagoya University.
Stable cell line generated: S2-NP cells and S2-NP-STAT92E cells were used.
Stable cell line generated: Stable S2 cell lines expressing tos and Su(var)205 were created.
Stable cell line generated: Stable S2 cell lines expressing polyglutamine repeats were generated.
Stable cell line generated: Stable S2 line expressing dCas9, dCas9-CBP, dCas9-CBP F2161A, or SAM (Synergistic Activation Mediator) were created.
GFP-α-tubulin expressing Drosophila S2 cells used.
Cells obtained from ExpreS2ion Biotechnologies.
Drosophila S2 (L2-4) cells were used.
S2 cells grown in the M. Gatti laboratory since 1997.
S2 cells were obtained from the laboratory of Linda Partridge.
S2 cells were obtained from the laboratory of Masayuki Miura.
S2 cells were obtained from the laboratory of Michael B. O'Connor (University of Minnesota).
S2 cells were obtained from the laboratory of Michael Boutros.
S2 cells were obtained from the laboratory of Qing Zhang.
S2 cells were obtained from the laboratory of R. Lehmann.
S2 cells were obtained from the laboratory of Sankar Maiti, IISER Kolkata, India.
S2 cells were originally from the W. Chia lab.
Stable cell line generated: A stable cell line containing a fusion of Mis12 and human Katanin p60 to specifically localize Katanin p60 to the kinetochore was produced.
Stable cell line generated: S2 cells that conditionally express crc were generated. S2 cells were obtained from the J. Hirst lab.
Stable cell line generated: S2 cells were stably transformed with inducible DptB tagged with Tag:polyHis.
Stable cell line generated: Stable S2 shams knock-out cell lines were generated.
Male cells were L2-4 cells, a clonal, tetraploid derivative of the S2 cell line that had been selected for euploidy in the lab of Patrick Heun.
S2 cells containing a stably integrated pMT-GAL4 construct were used.
S2 cells were obtained from the Henikoff laboratory.
S2 cells were obtained from the laboratory of Elizabeth Gavis.
Stable cell line generated: A set of three stable S2 cell lines carrying deletions that affect the upSET coding region were created.
Stable cell line generated: S2 cell lines stably expressing full-length as well as N-terminal, and C-terminal mutants of Sym were created.
An S2 cell line stably expressing Irbp18 tagged with Tag:proteinA-Tag:CS(TEVp)-Tag:FLAG was used.
S2 cells of unspecified origin.
S2 cells were obtained from the UCSF Cell Culture Facility.
S2 cells were obtained from the laboratory of R. Vale.
Stable cell line generated: A stable line containing a knockout allele of loqs was created.
Stable cell line generated: S2 cell lines stably expressing extracellular cadherin domains of shg tagged with GFP or Tag:polyHis or both were created.
S2 cells were obtained from the China Center for Type Culture Collection.
S2-NP cells were obtained from the laboratory of Norbert Perrimon.
Stable cell line generated: Stable S2 cell line created expressing His-FLAG-TAP tagged Clk.
S2 cells were obtained from the laboratory of R. Tanguay (Laval University).
Stable cell line generated: Stable S2 cell lines were created expressing eyg containing a mutation in the putative motif for interaction with Su(var)205.
A line that constitutively expresses GAL4 under the tubulin promoter (S2-tub-GAL4) was used.
Cells were obtained from the laboratory of Artavanis-Tsakonas.
Stable cell line generated: Stabel S2 cell lines expressing GFP with or without C-terminal Tag-V5-Tag:HIS-tagged Listericin were generated. A stable S2 cell line expressing inducible PGRP-LE was used.
"S2-L4" cells were used.
Stable cell line generated: A stable S2 cell line that constitutively expresses an Tag:HA-tagged Dredd variant in which the active site cysteine has been replaced with an alanine (HADreddC408A) were created. Stable S2 cell lines that constitutively express Tag:HA-tagged wild type or mutant dnr1 in which the essential RING domain active site cysteine was replaced with a tyrosine were also created (HADnr1 and HADnr1C563Y).
Stable cell line generated: An inducible stable S2 cell line expressing an Unc104(389)-mCherry-Pex26(245-345) construct was generated. Unc104 is from C elegans and Pex is from human. Human Pex26 is used as an artificial N-terminal motor tag and residues 245-305 are sufficient to target proteins onto peroxisomes.
Stable cell line generated: Inducible stable S2 cell lines were created overexpressing Gclc with in-frame Tag:V5 and Tag:polyHis.
Stable cell line generated: Stable S2 cell lines containing Tag:V5- and Tag:polyHis-tagged Prx5 were generated.
S2 cells stably transfected with human EGFR (S2(EGFR)) were obtained from the laboratory of David Baker.
Stable cell line generated: A stable cell line that expresses shg under the control of the Act5C promoter was established. Stable S2R+ cell lines expressing inducible wild type mbt (Tag:MYC-Mbt), a kinase-dead version (Tag:MYC-Mbt[T525A]) and a constitutively-activated mbt (Tag:MYC-Mbt[S492N/S521E]) were used.
Stable cell line generated: A stable S2 cell line expressing poloGFP was generated as well as stable lines expressing wild type or mutant forms of polo. In addition, stable lines expressing either Map205-Tag:MYC or the phospho-mimic mutants Map205-S283D-Tag:MYC and Map205-S283E-Tag:MYC were generated.
Stable cell line generated: A stable S2 cell lines expressing both GFP-alphaTub84B and inducible CID-mCherry was generated.
Stable cell line generated: An inducible stable S2 cell line was created that expresses epitope-tagged Smn. The "TagIt" epitope consists of the first 30 amino acids of human SMN protein.
Stable cell line generated: Inducible stable S2 cell lines were created expressing mtDNA-helicase, one of four deletion mutations of mtDNA-helicase, or one of eight mtDNA-helicase variants carrying the alanine substitutions K574A, R576A, Y577A, D580A, E587A, F588A, K590A, and Y595A.
Stable cell line generated: Inducible stable S2 cell lines were created expressing epitope-tagged wild type or mutant Kdm4A.
Stable cell line generated: Stable S2 cell lines were generated containing inducible Tag:V5-Tag:polyHis-ort or Tag:V5-Tag:polyHis-HisCl1.
Stable cell line generated: Inducible S2 cell lines were established that express GFP, GFP fused to a classic NLS (nuclear localization signal (cNLS-GFP), or GFP carrying a nuclear export signal (GFP-NES). Living cells expressing native GFP showed a homogenous distribution of the fluorescent signal. The cNLS-GFP reporter accumulates in nuclei, whereas the GFP-NES cargo localizes predominantly in the cytoplasm.
Stable cell line generated: Stable S2 cell lines were created expressing copper-inducible epitope-tagged exosome subunits to examine the subcellular distribution of exosome complex components. Stable lines were created containing the following exosome subunits; Dis3, Rrp6, Mtr3, Rrp42, Rrp4, Ski6, Rrp46, Rrp40, and Csl4.
Stable cell line generated: Stable S2 cell lines were created expressing GFP-tagged DCTN1-p150 or GFP-tagged αTub84B.
Stable cell line generated: Stable S2 cell lines were created expressing EGFP and polyQ.
Stable cell line generated: Stable S2 cell lines were created containing an HRE (hypoxia-inducible factor responsive element)-pLUC reporter.
Stable cell line generated: Stable S2 cell lines were created expressing either Ecol\lacZDptA.PR or RelMtnA.EGFP.
Stable cell line generated: S2 cell lines stably transfected with various Tl constructs containing mutations at conserved residues were established.
Stable cell line generated: The PC-FH S2 cell line was created that expresses Pc protein with both Tag:FLAG epitope and Tag:polyHis at its C terminus.
! TC9. Comment(s) (free text) :S2 cells were obtained from the laboratory of Thomas Bunch.
S2 cells stably expressing Rdl were used.
Stable cell lines generated: Stable cell lines that express the full length spi protein (designated S2:spi ) or a truncated, secreted form of spi protein (designated S2:sspi ) were created. Stable cell lines that express Egfr were created and designated S2:DER1b and S2:DER2f . The S2:DER2f cell line is a constitutive Egfr-expressing cell line that was subsequently called D2F.
Stable cell lines were created containing Nrg constructs. They are designated S2:pRmHa3-nrg [167] (short isoform), S2:pRmHa3-nrg [180] (long isoform), and S2:pRmHa3-nrg [GPI] (containing the first 1121 amino acids of Nrg fused to the 53 C-terminal amino acids of Fas1 containing the GPI attachment signal.
S2 cells were obtained from the laboratory of Phil Beachy.