FlyBase Cell Line Report: S2-unspecified

General Information

Symbol

S2-unspecified

Species

D. melanogaster

Feature type

cultured cell-line

FlyBase ID

FBtc9000001

Source

Tissue Source

embryo

(Cherbas, 2016.3.25)

Developmental Stage

late embryonic stage

(Cherbas, 2016.3.25)

Lab of Origin

Characterization

Sex

Karyotype

Integrated Constructs (0)

Comments

Cell lines with GFP or Tag:FLAG inserted into the ush or Mi-2 endogenous locus were created.

(Lenz et al., 2021)

S2 cells stably expressing hh-N were obtained from the laboratory of J.Jiang.

(Kim et al., 2021)

S2 cells were obtained from the laboratory of Dr. H. Siomi.

(Tanaka et al., 2021)

S2[Cas9] cells were obtained from the laboratory of Klaus Forstemann.

(Lenz et al., 2021, Mačinković et al., 2019)

Stable cell line generated: Drosophila codon optimized or deoptimized firefly luciferase expressed under the control of various promoters.

(Yang et al., 2021)

Stable cell line generated:DMEL cells expressing GFP-tagged mv under the contol of the MT promoter were created.

(Lattao et al., 2021)

Stable cell line generated:Stable S2 Mettl3-KO cell lines were generated by CRISPR/Cas9-mediated mutatgenesis.

(Kan et al., 2021)

A scra- FBto0000118:mCherry S2 cell line was used.

(Bai et al., 2020)

S2 cells were obtained from Expression Systems, 94-005F.

(Alameh et al., 2020)

S2 cells were obtained from the laboratory of Don Rio.

(Jain et al., 2020)

S2 cells were obtained from the laboratory of P. Zamore.

(Xie et al., 2020)

Stable cell line generated: A stable S2 cell line expressing 3XHA-tagged Nipped-A was created.

(Mahesh et al., 2020)

Stable cell line generated: A stable S2 cell line was created that expresses SCAT3m a FRET-based caspase activity indicator.

(Fujisawa et al., 2020)

Stable cell line generated: An S2 cell line expressing cic tagged with Tag:SBP was created. S2 cells were obtained from the laboratory of S. Artavanis-Tsakonas.

(Paul et al., 2020)

Stable cell line generated: CRISPR/Cas9 used to tag endogenous Spt6 with GFP; stably transfected versions of the resulting Spt6-GFP cell line were generated with a small molecule inducible version of the geGradeFP system, a tamoxifen inducible HA-cid and/or a version of cid capable of recombination induced tag exchange (RITE).

(Bobkov et al., 2020)

Stable cell line generated: S2 cells expressing GFP tagged Slik, or fragments of Slik, under the control of Mt promoter.

(De Jamblinne et al., 2020)

Stable cell line generated: S2 cells expressing Tag:HA -tagged mtSSB and Tag:V5 -taggeds rnh1 were created.

(González de Cózar et al., 2020)

Stable cell line generated: S2 cells expressing FLAG-tagged deletion forms of BigH1.

(Climent-Cantó et al., 2020)

Stable cell line generated: Stable S2 cell line expressing FLAG-tagged human EZHIP under the control of the MT promoter was created.

(Jain et al., 2020)

Stable cell line generated: Two stable S2 cell lines were created that are knockouts for lncRNA:Vinr.

(Zhang et al., 2020)

Stable cell line generated:A stable cell line expressing Tag:FLAG-tagged blanks under the control of the "MtnDE" promoter was created.

(Nitschko et al., 2020)

Stable cell line generated:S2 cells expressing FLAG tagged PIG-B or biotin ligase, miniTurboID, conjugated and V5 tagged Lam.

(Yamamoto-Hino et al., 2020)

Stable cell line generated:Stable cell lines expressing 3XTag:FLAG-tagged isoforms of dom were generated. The dom-PA and dom-PE were specifically targeted.

(Scacchetti et al., 2020)

Stable cell lines generated: Clonal S2 cells harboring mutations in CG34401 were created.

(Shi et al., 2020)

Stable cell lines generated:S2 cells expressing fne or fne fused to the RNA editing enzyme Adar tagged with Tag:V5 under the control of the MT promoter were created.

(Alizzi et al., 2020)

Cells obtained from RIKEN Bioresource Center.

(Ando et al., 2019)

L2-4 (S2 subclone, provided by P. Heun).

(Albig et al., 2019)

S2 cells expressing GFP-Utrophin-CH/GFP-Tubby and mCherry-tubulin and S2 cells expressing anilllin-mCherry and GFP-Spaghetti-squash from the laboratory of Gilles Hickson were used.

(Carim et al., 2019)

S2 cells were obtained from the laboratory of Alain Debec.

(Duarte Junior et al., 2019)

S2 cells were obtained from the laboratory of Erjun Ling.

(Sun et al., 2019)

S2 cells were obtained from the laboratory of F. Feiguin.

(Bertolio et al., 2019)

S2 cells were obtained from the laboratory of J.H. Kim.

(Moyer and Holland, 2019)

S2 cells were obtained from the laboratory of Mikiko Siomi (University of Tokyo).

(Kobayashi et al., 2019, Kobayashi et al., 2019)

S2 cells were obtained from the laboratory of N. Silverman.

(Shigemura et al., 2019, de Toeuf et al., 2018)

S2 cells were obtained from the laboratory of Patrick Emery.

(Gu et al., 2019)

S2 cells were obtained from the laboratory of Sebastien Carreno.

(Carim et al., 2019)

Stable S2 cell lines expressing Ada2b isoform B were used.

(Soffers et al., 2019)

Stable cell line generated: A density-responsive reporter system was characterized in S2 cells using cells stably transfected with "MG" (MT-GFP) and "MR" (CA-MT-eve-RFP) reporters run by the eve, Adh, or Hsp70 promoters.

(Romine et al., 2019)

Stable cell line generated: A stable S2 cell line was created containing a construct expressing GFP under the control of the Ppa promoter (nucleotide position +1 to -1000).

(Moreno-Moreno et al., 2019)

Stable cell line generated: A stable cell line coexpressing EGFP-α-tubulin and γ-tubulin-Tag-RFP-T was created. A stable cell line coexpressing EGFP-α-tubulin, γ-tubulin-Tag-RFP-T, and mTurquoise2-dgt5 was created.

(Verma and Maresca, 2019)

Stable cell line generated: An inducible CRISPR/Cas9 system was developed that generates double strand breaks in the 11- to 12-base-pair (bp) dodeca satellite tandem repeats located in the chromosome 3 pericentromere.

(Janssen et al., 2019)

Stable cell line generated: S2 cell lines were generated expressing various sti-GFP constructs.

(El-Amine et al., 2019)

Stable cell line generated: S2 cells stably expressing ken-CycB-GFP/mCherry-α-tubulin or GFP-aurB/WTCycB-mCherry were created.

(Afonso et al., 2019)

Stable cell line generated: S2 cells stably expressing a pcs construct ( myc::APEX2::Pcs ) were generated. S2 cells were obtained from the laboratory of Dr. Gota Goshima at Nagoya University.

(Otsuka et al., 2019)

Stable cell line generated: S2-NP cells and S2-NP-STAT92E cells were used.

(Kim et al., 2019)

Stable cell line generated: Stable S2 cell lines expressing tos and Su(var)205 were created.

(Janssen et al., 2019)

Stable cell line generated: Stable S2 cell lines expressing polyglutamine repeats were generated.

(Lin et al., 2019)

Stable cell line generated: Stable S2 cell lines were created expressing Clk-Tag:HA/Tag:FLAG or cyc-Tag:V5.

(Cho et al., 2019)

Stable cell line generated: Stable S2 cell lines were generated carrying knockout and/or overexpression allels of AGO1, AGO2 and Nbr.

(Reichholf et al., 2019)

Stable cell line generated: Stable S2 line expressing dCas9, dCas9-CBP, dCas9-CBP F2161A, or SAM (Synergistic Activation Mediator) were created.

(Sajwan and Mannervik, 2019)

GFP-α-tubulin expressing Drosophila S2 cells used.

(Ye et al., 2018)

Cells obtained from ExpreS2ion Biotechnologies.

(Cosmanescu et al., 2018)

Drosophila S2 (L2-4) cells were used.

(Zouaz et al., 2018)

S2 cells grown in the M. Gatti laboratory since 1997.

(Strunov et al., 2018, Strunov et al., 2016)

S2 cells stably integrated with an inducible dhd-EGFP construct were used.

(Petrova et al., 2018)

S2 cells stably overexpressing vig-V5 and vig2-V5 proteins were used.

(Tsui et al., 2018)

S2 cells were obtained from the laboratory of Linda Partridge.

(Ramani et al., 2018)

S2 cells were obtained from the laboratory of Masayuki Miura.

(Arii et al., 2018)

S2 cells were obtained from the laboratory of Michael B. O'Connor (University of Minnesota).

(Okamoto et al., 2018)

S2 cells were obtained from the laboratory of Michael Boutros.

(Moraru et al., 2018)

S2 cells were obtained from the laboratory of Qing Zhang.

(Lee et al., 2018)

S2 cells were obtained from the laboratory of R. Lehmann.

(Astigarraga et al., 2018)

S2 cells were obtained from the laboratory of Sankar Maiti, IISER Kolkata, India.

(Bar et al., 2018)

S2 cells were originally from the W. Chia lab.

(Koe et al., 2018)

Stable S2 Hen1 knockout cells were used as well as Hen1 KO cells expressing Ath-HEN1 (HEN1 from Arabadopsis thaliana).

(Alberti et al., 2018)

Stable cell line generated: A stable S2 cell line expressing pnut tagged with Tag:FLAG was generated.

(Akhmetova et al., 2018)

Stable cell line generated: A stable cell line containing a fusion of Mis12 and human Katanin p60 to specifically localize Katanin p60 to the kinetochore was produced.

(Advani et al., 2018)

Stable cell line generated: S2 cell lines containing EGFP-tagged Orc2, Orc5, Mcm2, or Mcm5 were generated.

(Sureka et al., 2018)

Stable cell line generated: S2 cells stably expressing an inducible Tag:MYC-tagged smo transgene under the control of the metallothionein promoter (pMT-Myc-Smo) were created. S2 cells stably expressing the N-terminal fragment of hhh protein (HhN) that is active for signaling were created.

(Li et al., 2018)

Stable cell line generated: S2 cells that conditionally express crc were generated. S2 cells were obtained from the J. Hirst lab.

(Malzer et al., 2018)

Stable cell line generated: S2 cells were stably transfected with yki and sd constructs.

(Vissers et al., 2018)

Stable cell line generated: S2 cells were stably transformed with inducible DptB tagged with Tag:polyHis.

(Barajas-Azpeleta et al., 2018)

Stable cell line generated: Stable S2 shams knock-out cell lines were generated.

(Pandey et al., 2018)

Stable cell line generated: Stable cell lines harbouring inducible Mt2-Tag:FLAG or Nsun2-Tag:FLAG-Tag:HA wild type and mutant constructs were established.

(Genenncher et al., 2018)

Stable cell line generated: Stable cell lines were generated to express one of the three Dmel globin genes, glob1, glob2, or glob3 as GFP fusion constructs.

(Burmester et al., 2018)

Male cells were L2-4 cells, a clonal, tetraploid derivative of the S2 cell line that had been selected for euploidy in the lab of Patrick Heun.

(Schunter et al., 2017)

S2 cells containing a stably integrated pMT-GAL4 construct were used.

(Toegel et al., 2017)

S2 cells were obtained from the Henikoff laboratory.

(Ramachandran et al., 2017)

S2 cells were obtained from the laboratory of Elizabeth Gavis.

(Johnson et al., 2017)

Stable cell line generated: A set of three stable S2 cell lines carrying deletions that affect the upSET coding region were created.

(McElroy et al., 2017)

Stable cell line generated: A stable S2 cell line expressing BigH1 :Tag:FLAG was generated.

(Carbonell et al., 2017)

Stable cell line generated: A stable S2 cell line was isolated that expresses the smoIP fluorescence-based sensor under the control of UAS and reproducibly responds to hh stimulation.

(Albert and Bökel, 2017)

Stable cell line generated: A stable inducible cell line expressing a catalytically inactive ClpP mutant (S214A) in which the conserved serine in the proteolytic active site was replaced with alanine was created. An inducible cell line capable of overexpressing bsf was established.

(Matsushima et al., 2017)

Stable cell line generated: An S2 cell line stably expressing pMT-N (full-length N under the control of the MtnA promoter) was created.

(Shukla et al., 2017)

Stable cell line generated: S2 cell lines stably expressing full-length as well as N-terminal, and C-terminal mutants of Sym were created.

(Harrington et al., 2017)

Stable cell line generated: Stable cell lines expressing GFP-Rz-His3 (Rz is the hammerhead-ribozyme-based mRNA reporter that produces nonstop mRNA fragments) or GFP-His3 were established. A stable cell line that expresses GFP-ptc-His3 under the control of the Act5C promoter was created.

(Hashimoto et al., 2017)

A stable cell line expressing enok tagged with Tag:FLAG was used.

(Huang et al., 2016)

An S2 cell line stably expressing Irbp18 tagged with Tag:proteinA-Tag:CS(TEVp)-Tag:FLAG was used.

(Francis et al., 2016)

S2 cells of unspecified origin.

(Cherbas, 2016.3.25)

S2 cells were obtained from the laboratory of R. Vale.

(Wang et al., 2016)

Stable cell line generated: A stable line containing a knockout allele of loqs was created.

(Lim et al., 2016)

Stable cell line generated: S2 cell lines stably expressing extracellular cadherin domains of shg tagged with GFP or Tag:polyHis or both were created.

(Nishiguchi et al., 2016)

Stable cell line generated: Stable S2 cell lines expressing wild type or mutant Khc tagged with GFP were created.

(Winding et al., 2016)

Stable cell line generated: Stable cell lines stably expressing inducible wild type and mutant (D228A) constructs of Tag:FLAG-tagged Csas were created.

(Mertsalov et al., 2016)

Stable cell line generated: expressing Tag:SBP tagged cic.

(Yang et al., 2016)

S2 cells were obtained from the China Center for Type Culture Collection.

(Wang et al., 2015)

Stable cell line generated: Stable S2 cell lines expressing wild type or variant l(2)gl-GFP and mCherry-tubulin were created. An S2 cell line expressing mCherry and GFP-tubulin was used.

(Carvalho et al., 2015)

Stable cell lines generated: A stable S2 cell line was created expressing cal1 tagged with GFP and lacI under the control of a Cu2+ inducible promoter.

(Chen et al., 2015)

S2-NP cells were obtained from the laboratory of Norbert Perrimon.

(Maimon et al., 2014)

Stable cell line generated: S2 cell lines expressing His2B GFP/mCherry-α-tubulin or GFP-aurB/mCherry-α-tubulin were created.

(Afonso et al., 2014)

Stable cell line generated: Stable S2 cell line created expressing His-FLAG-TAP tagged Clk.

(Mahesh et al., 2014)

S2 cells stably expressing N tagged with EGFP or Ser tagged with tdTomato were used.

(Fleming et al., 2013)

S2 cells were obtained from the laboratory of R. Tanguay (Laval University).

(Gareau et al., 2013)

Stable cell line generated: S2 cell lines coexpresing scra-GFP and wild type or kinase-dead versions of sti were generated.

(El Amine et al., 2013)

Stable cell line generated:S2 cells were stably transfected with BEAF-32 ::mCherry and su(Hw) ::EGFP.

(Schoborg et al., 2013)

Stable cell line generated: Stable S2 cell lines were created expressing eyg containing a mutation in the putative motif for interaction with Su(var)205.

(Salvany et al., 2012)

A line that constitutively expresses GAL4 under the tubulin promoter (S2-tub-GAL4) was used.

(Parisi et al., 2011)

Cells were obtained from the laboratory of Artavanis-Tsakonas.

(Leiserson et al., 2011)

Stable cell line generated: A stable S2 cell line expressing an inducible cbt-Tag:V5 fusion protein was generated.

(Belacortu et al., 2011)

Stable cell line generated: Stable cell lines were generated expressing GFP-Ocrl, mCherry-Ocrl, GFP-Ocrl-PD (phosphatase dead), GFP-Hsap\UTRN, Tubulin-GFP or GFP-Tb.

(Ben El Kadhi et al., 2011)

Stable cell line generated: A stable S2 cell line that contains inducible EGFP-tagged cid was created.

(Podhraski et al., 2010)

Stable cell line generated: S2 cell lines were created that express EGFP mRNA containing target sites in the 3' UTR for either mir-1, mir-34, mir-let7, or mir-ban.

(Ameres et al., 2010)

Stable cell line generated: Stabel S2 cell lines expressing GFP with or without C-terminal Tag-V5-Tag:HIS-tagged Listericin were generated. A stable S2 cell line expressing inducible PGRP-LE was used.

(Goto et al., 2010)

Stable cell line generated: Stable S2 cell lines expressing inducible wild type or deletion constructs of fra tagged with Tag:MYC or Tag:HA were generated.

(Dorsten et al., 2010)

Stable cell lines used: S2 cells stably expressing if or mys subunits were utilized.

(Fraichard et al., 2010)

"S2-L4" cells were used.

(Raker et al., 2009)

Stable cell line generated: dco[WT], inducible Tag:MYC-tagged, dco^S, dco^L, the catalytically inactive dco[K38R] protein, or truncated forms of dco were used to produce stably transfected S2 cell lines.

(Fan et al., 2009)

Stable cell line generated: hh reporter cell lines were stably transfected with tub-Ci, ptc-pLUC, tub-Rren\LUC or pMT-hhN.

(Mosimann et al., 2009)

Stable cell line generated: A stable S2 cell line expressing Tag:FLAG-tagged KLHL18 was generated.

(Fujiyama-Nakamura et al., 2009)

Stable cell line generated: A stable S2 cell line that constitutively expresses an Tag:HA-tagged Dredd variant in which the active site cysteine has been replaced with an alanine (HADreddC408A) were created. Stable S2 cell lines that constitutively express Tag:HA-tagged wild type or mutant dnr1 in which the essential RING domain active site cysteine was replaced with a tyrosine were also created (HADnr1 and HADnr1C563Y).

(Guntermann et al., 2009)

Stable cell line generated: A stable S2 cell lines expressing inducible Tag:HA-tagged HPS4 was generated.

(Lee et al., 2009)

Stable cell line generated: An S2 cell line stably coexpressing Mtor-mCherry and GFP-αTub84B was generated. S2 cells stably coexpressing GFP-αTub84B and mRFP-mad2 were used. GFP-αTub84B was obtained from the laboratory of R. Vale.

(Lince-Faria et al., 2009)

Stable cell line generated: An inducible stable S2 cell line expressing an Unc104(389)-mCherry-Pex26(245-345) construct was generated. Unc104 is from C elegans and Pex is from human. Human Pex26 is used as an artificial N-terminal motor tag and residues 245-305 are sufficient to target proteins onto peroxisomes.

(Ally et al., 2009)

Stable cell line generated: Inducible stable S2 cell lines were created overexpressing Gclc with in-frame Tag:V5 and Tag:polyHis.

(Radyuk et al., 2009)

Stable cell line generated: Inducible stable S2 cell lines were established that express full length Tag:V5-tagged Rrp4, Rrp6, or Ski6.

(Hessle et al., 2009)

Stable cell line generated: S2 hh-reporter cells were stably transfected with tubulin-ci, ptc-pLUC, tubulin Rren\LUC, and pMT-hhN.

(Mosimann et al., 2009)

Stable cell line generated: S2 cells were stably transfected with inducible Tag:FLAG-tagged full-length as well as N-terminally and C-terminally truncated Pbp95 constructs. Each was co-transfected with complementary full length Pbp45 and Pbp49@ constructs.

(Hung et al., 2009)

Stable cell line generated: Stable S2 cell lines containing Tag:V5- and Tag:polyHis-tagged Prx5 were generated.

(Radyuk et al., 2009)

Stable cell line generated: Stable S2 cell lines expressing HP1c tagged with Tag:FLAG were established.

(Abel et al., 2009)

Stable cell line generated: Stable S2 cell lines expressing TAG-FLAG-tagged Ada2b (isoform Ada2b-RB), Sgf29, Saf6, P32, wda, Ada1-2, and TAF6 were generated.

(Weake et al., 2009)

Stable cell line generated: Stable S2 cell lines were generated containing inducible csul, icln, CG4038. All are tagged with the affinity tag pMTagIt.

(Kroiss et al., 2009)

Stable cell line generated: Stable cell lines were generated starting from a S2+ line that stably expresses Egfr. This line was stably transformed with full length or mutant Ptp10D.

(Jeon and Zinn, 2009)

Stable cell line generated: Stable inducible S2 cell lines expressing Pld tagged with Tag:MYC were established.

(Raghu et al., 2009)

Stable cell line generated: Three stable S2 cell lines were generated. Each contains EGFP-alpha-tubulin under the control of the Act5C promoter along with either wild type, S573A mutant or S573E mutant Disc\RFP-Klp10A under the control of the MtnA promoter.

(Mennella et al., 2009)

Cells stably expressing inducible GFP-Rel were used.

(Lee et al., 2008)

S2 cells expressing GFP-tagged αTub84B were obtained from the Sharp laboratory (Albert Einstein).

(Farzan et al., 2008)

S2 cells expressing mitoXhoI (which encodes XhoI fused to a mitochondrial targeting leader at its N-terminus and Tag:MYC at its C-terminus) under the control of a MtnA promoter were used.

(Xu et al., 2008)

S2 cells stably transfected with human EGFR (S2(EGFR)) were obtained from the laboratory of David Baker.

(Krishnamoorthy, 2008)

Stable S2 cells expressing rpr under the control of the MtnA promoter (S2-Mt-reaper) were used.

(Bardet et al., 2008)

Stable cell line generated: A stable cell line that expresses shg under the control of the Act5C promoter was established. Stable S2R+ cell lines expressing inducible wild type mbt (Tag:MYC-Mbt), a kinase-dead version (Tag:MYC-Mbt[T525A]) and a constitutively-activated mbt (Tag:MYC-Mbt[S492N/S521E]) were used.

(Menzel et al., 2008)

Stable cell line generated: A stable S2 cell line expressing polo^GFP was generated as well as stable lines expressing wild type or mutant forms of polo. In addition, stable lines expressing either Map205-Tag:MYC or the phospho-mimic mutants Map205-S283D-Tag:MYC and Map205-S283E-Tag:MYC were generated.

(Archambault et al., 2008)

Stable cell line generated: A stable S2 cell line that overexpresses Mvl tagged with tag:FLAG was generated.

(Southon et al., 2008)

Stable cell line generated: A stable S2 cell lines expressing both GFP-alphaTub84B and inducible CID-mCherry was generated.

(Coelho et al., 2008)

Stable cell line generated: An inducible stable S2 cell line was created that expresses epitope-tagged Smn. The "TagIt" epitope consists of the first 30 amino acids of human SMN protein.

(Kroiss et al., 2008)

Stable cell line generated: Inducible stable S2 cell lines were created expressing Tag:FLAG-tagged fragments of the ds extracellular domains or secreted, epitope-tagged mutant or wild type fj.

(Ishikawa et al., 2008)

Stable cell line generated: Inducible stable S2 cell lines were created expressing mtDNA-helicase, one of four deletion mutations of mtDNA-helicase, or one of eight mtDNA-helicase variants carrying the alanine substitutions K574A, R576A, Y577A, D580A, E587A, F588A, K590A, and Y595A.

(Matsushima et al., 2008)

Stable cell line generated: Inducible stable S2 cell lines were created expressing epitope-tagged wild type or mutant Kdm4A.

(Lin et al., 2008)

Stable cell line generated: S2 cell lines expressing mutant mle proteins tagged with Tag:FLAG under the control of the MtnA promoter were generated.

(Morra et al., 2008)

Stable cell line generated: Stable S2 cell lines expressing Mob4-GFP under the control of the MtnA promoter were generated.

(Trammell et al., 2008)

Stable cell line generated: Stable S2 cell lines expressing scra-GFP were generated.

(Hickson and O'Farrell, 2008)

Stable cell line generated: Stable S2 cell lines were created containing UAS constructs with either full length H or H variants with mutated binding sites for gro, CtBP, or both. The stable lines also contain pMT-GAL4.

(Protzer et al., 2008)

Stable cell line generated: Stable S2 cell lines were generated containing inducible Tag:V5-Tag:polyHis-ort or Tag:V5-Tag:polyHis-HisCl1.

(Pantazis et al., 2008)

The following stable S2 cell lines were used: Dl-WTNdeMYC, fng-GFP, Sar1[H74G]-GFP or GFP-Sec23.

(Ivan et al., 2008)

S2 cells stably expressing GFP-αTub84B were used.

(Orr et al., 2007)

S2 cells stably expressing GFP-tagged Rel under the control of the MtnA promoter were obtained from the laboratory of E. Foley.

(Dijkers and O'Farrell, 2007)

Stable cell line generated: Inducible S2 cell lines were established that express GFP, GFP fused to a classic NLS (nuclear localization signal (cNLS-GFP), or GFP carrying a nuclear export signal (GFP-NES). Living cells expressing native GFP showed a homogenous distribution of the fluorescent signal. The cNLS-GFP reporter accumulates in nuclei, whereas the GFP-NES cargo localizes predominantly in the cytoplasm.

(Sabri et al., 2007)

Stable cell line generated: S2 cells stably expressing a Pol III-specific subunit RpIII128 tagged with Tag:V5 at the C-terminus were generated.

(Isogai et al., 2007)

Stable cell line generated: S2 cells were stably transfected with CTCF flagged with Tag:FLAG.

(Mohan et al., 2007)

Stable cell line generated: S2 cells were stably transfected with QC or isoQC cDNA sequences.

(Schilling et al., 2007)

S2 cells stably expressing GFP-tagged pav or tum under the control of the Act5C promoter were generated. In addition lines containing GFP-tagged tum with human CD8 attached or just CD8-GFP under the control of the metallothionein promoter were generated.

(D'Avino et al., 2006)

Stable cell line generated: S2 cells were stably transfected with copper-inducible Taf1 tagged with Tag:HA.

(Katzenberger et al., 2006)

Stable cell line generated: Stable S2 cell lines were created expressing a luciferase construct that can be targeted by mir-2b. Lamp1-GFP S2 cells from the laboratory of R. Vale and S2 cells expressing GFP-Rel were also utilized.

(Saleh et al., 2006)

Stable cell line generated: Stable S2 cell lines were created expressing copper-inducible epitope-tagged exosome subunits to examine the subcellular distribution of exosome complex components. Stable lines were created containing the following exosome subunits; Dis3, Rrp6, Mtr3, Rrp42, Rrp4, Ski6, Rrp46, Rrp40, and Csl4.

(Graham et al., 2006)

Stable cell line generated: Stable S2 cell lines were created expressing GFP-tagged DCTN1-p150 or GFP-tagged αTub84B.

(Delcros et al., 2006)

Stable cell line generated: S2 cells were stably transfected with sca-GFP.

(Mok et al., 2005)

Stable cell line generated: S2 cells were stably transfected with a Notum-pLUC construct, which serves as a reporter to monitor wg signalling.

(Staedeli and Basler, 2005)

Stable cell line generated: Stable S2 cell lines were created expressing EGFP and polyQ.

(Nelson et al., 2005)

Stable cell line generated: Stable S2 cell lines were created containing an HRE (hypoxia-inducible factor responsive element)-pLUC reporter.

(Dekanty et al., 2005)

Stable cell line generated: Stable S2 cell lines were created expressing either Ecol\lacZ^DptA.PR or Rel^MtnA.EGFP.

(Foley and O'Farrell, 2004)

Stable cell line generated: S2 cells were stably transfected with a copper-inducible tor-pll construct.

(Leulier et al., 2003)

Stable cell line generated: Stable S2 cell lines were created to over-express Prx3 or Jafrac1.

(Radyuk et al., 2003)

Stable cell line generated: S2 cell lines stably transfected with various Tl constructs containing mutations at conserved residues were established.

(Ooi et al., 2002)

Stable cell line generated: The PC-FH S2 cell line was created that expresses Pc protein with both Tag:FLAG epitope and Tag:polyHis at its C terminus.

(Huang et al., 2002)

! TC9. Comment(s) (free text) :S2 cells were obtained from the laboratory of Thomas Bunch.

(Lansdell and Millar, 2000, Lansdell et al., 1997)

Stable cell lines generated: Stable cell lines that express the full length spi protein (designated S2:spi ) or a truncated, secreted form of spi protein (designated S2:sspi ) were created. Stable cell lines that express Egfr were created and designated S2:DER1b and S2:DER2f . The S2:DER2f cell line is a constitutive Egfr-expressing cell line that was subsequently called D2F.

(Schweitzer et al., 1995)

Stable cell lines were created containing Nrg constructs. They are designated S2:pRmHa3-nrg [167] (short isoform), S2:pRmHa3-nrg [180] (long isoform), and S2:pRmHa3-nrg [GPI] (containing the first 1121 amino acids of Nrg fused to the 53 C-terminal amino acids of Fas1 containing the GPI attachment signal.

(Hortsch et al., 1995)

S2 cells were obtained from the laboratory of Phil Beachy.

(Winslow et al., 1989)

Parental Lines (0)

Descendant Lines (4)

Cloned from S2-unspecified:

S2-B2
S2c1

Transformant of S2-unspecified:

529SU
D2F

External Stocks and Resources ( 0 )

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

CVCL_Z232

(Bairoch, 2016)

D-Mel-2

(Creagh et al., 2009)

D-Mel2

(Wang et al., 2016)

DMEL

(Lattao et al., 2021)

Dmel-2

(Sullivan et al., 2009)

Dmel2

(Loedige et al., 2015, Eissenberg et al., 2011)

(Gür et al., 2020, Huijbregts et al., 2009, Balasov et al., 2007)

RRID: CVCL_Z232

(Metwally et al., 2021)

RRID:CVCL_Z232

(Bairoch, 2016)

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S2 Schneider cell line

(Mazina et al., 2016, Mazina et al., 2016)

S2 Schneider cells

(Zandany et al., 2015)

S2+mtDer

(Jeon and Zinn, 2009)

S2- Dmel2

(Samata et al., 2020)

S2-unspecified

(Cherbas, 2016.3.25)

SL2

(Leggio et al., 2018, Kramer et al., 2015, Panda et al., 2014, Doggett et al., 2011, Gelbart et al., 2009, Hilger et al., 2009, Alekseyenko et al., 2008, Straub et al., 2008, Doumanis et al., 2007, Larschan et al., 2007, Rasheva et al., 2006, Gesellchen et al., 2005, Rehwinkel et al., 2005, Jin et al., 2004, Pile et al., 2002, Doumanis et al., 2001, Zhang et al., 2001)

Schneider

(Mazina et al., 2018, Nappi et al., 2005)

Schneider 2

(Wang et al., 2018, Bairoch, 2016)

d.mel-2

(Garrido et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1,718)

Report Sections

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