FlyBase Insertion Report: Dmel\P{GawB}D42

General Information

Symbol

Dmel\P{GawB}D42

Species

D. melanogaster

Name

FlyBase ID

FBti0002759

Feature type

transposable_element_insertion_site

Also Known As

D42-Gal4, D42, D42 Gal4, D42Gal4, GAL4-D42, P[GawB]D42, D42:Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, Toll-6

Viability / fertility

viable, fertile

Associated allele(s)

Scer\GAL4^Toll-6-D42

Stock availability

4 publicly available

Genomic Location

Chromosomal Location

3L ( 71C2 )

Sequence Location

3L:15,329,625..15,329,625

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

(Kololdziej, 2007.10.30)

Sequence Location

3L:15,329,625..15,329,625

(Sanyal, 2009)

Orientation

Cytological location (computed by FlyBase)

71C2 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Insertion location reported at 3L:15322725 in R5 coordinates, 7075 bp upstream of the transcription start site of Toll-6.

(Sanyal, 2009)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Beck et al., 2015, Vasmer et al., 2014, Vonhoff et al., 2013, Bayat et al., 2012, Beck et al., 2012, Couthouis et al., 2012, Djagaeva et al., 2012, Füger et al., 2012, Gregory et al., 2012, Hazelett et al., 2012, Lee et al., 2012, Mozer and Sandstrom, 2012, Nechipurenko and Broihier, 2012, Pradhan et al., 2012, Ruiz et al., 2012, Ryglewski et al., 2012, Tsai et al., 2012, Xia et al., 2012, Chun-Jen Lin et al., 2011, Couthouis et al., 2011, Estes et al., 2011, Freeman et al., 2011, James and Broihier, 2011, Jepson et al., 2011, Keegan et al., 2011, Lin et al., 2011, Liu et al., 2011, Rossetto et al., 2011, Schultz et al., 2011, Stavropoulos and Young, 2011, Zhong et al., 2011, Bahadorani et al., 2010, Batlevi et al., 2010, Boerner and Duch, 2010, Cowan et al., 2010, Crocker et al., 2010, Elden et al., 2010, Khuong et al., 2010, Lone et al., 2010, Lorenzo et al., 2010, Melicharek et al., 2010, Nedelsky et al., 2010, Owald et al., 2010, Pathak et al., 2010, Radyuk et al., 2010, Shidara and Hollenbeck, 2010, Voigt et al., 2010, Vonhoff and Duch, 2010, Barber et al., 2009, Dason et al., 2009, Feiguin et al., 2009, Fischer et al., 2009, Kissler et al., 2009, Li and Tower, 2009, Martin et al., 2009, Orso et al., 2009, Sanyal, 2009, Barkus et al., 2008, Cauchi et al., 2008, Chai et al., 2008, Kwak et al., 2008, Legan et al., 2008, Watson et al., 2008, Ford et al., 2007, Haghnia et al., 2007, Huang and Haddad, 2007, Llorens et al., 2007, Romero-Pozuelo et al., 2007, Wu et al., 2007, Grammenoudi et al., 2006, Lind et al., 2006, Pilling et al., 2006, Schroll et al., 2006, Walker, 2006, Walker et al., 2006, Anderson et al., 2005, Hebbar and Fernandes, 2005, Horiuchi et al., 2005, Houseley et al., 2005, Miller et al., 2005, Orr et al., 2005, Verstreken et al., 2005, Yoshihara et al., 2005, Zheng et al., 2005, Broughton et al., 2004, Mudher et al., 2004, Rogina and Helfand, 2004, Kuniyoshi et al., 2003, Landgraf et al., 2003, Missirlis et al., 2003, Morey et al., 2003, Chan et al., 2002, Marek and Davis, 2002, Nitz et al., 2002, Seroude et al., 2002, Song et al., 2002, Sweeney and Davis, 2002, Taghert et al., 2001, Wittmann et al., 2001, Shayan and Atwood, 2000, Usui-Aoki et al., 2000, Cheung et al., 1999, Parkes et al., 1998, Gustafson and Boulianne, 1996, Yeh et al., 1995)

Toll-6

(Sanyal, 2009)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^Toll-6-D42

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

motor neuron

(Yeh et al., 1995)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

late embryonic stage

(Gustafson and Boulianne, 1996)

larval stage

motor neuron

(Hazelett et al., 2012, Nitz et al., 2002)

third instar larval stage

embryonic/larval motor neuron

(Coyne et al., 2014)

embryonic/larval salivary gland

(Ruppert et al., 2017)

larval ventral nerve cord

(Sanyal, 2009)

larval brain

(Sanyal, 2009)

motor neuron

(Sanyal, 2009)

larval RP2 motor neuron

(Sanyal, 2009)

larval DA1 motor neuron

(Sanyal, 2009)

sensory neuron

(Sanyal, 2009)

pupal stage P6 -- pharate adult stage P15

indirect flight muscle motor neuron DLMN5

(Vonhoff et al., 2013)

pharate adult stage P8

antennal lobe glomerulus DA1

(Ward et al., 2015)

antennal lobe glomerulus VA1d

(Ward et al., 2015)

antennal lobe glomerulus VA1v

(Ward et al., 2015)

antennal lobe glomerulus DL3

(Ward et al., 2015)

antennal lobe glomerulus DC3

(Ward et al., 2015)

antennal lobe glomerulus DC1

(Ward et al., 2015)

antennal lobe glomerulus DA4l

(Ward et al., 2015)

antennal lobe glomerulus DA4m

(Ward et al., 2015)

Additional Information

Statement

Reference

Drives expression in motor neurons.

(FlyBase Curators, 2017)

ScerGAL4^Toll-6-D42 drives expression in glomeruli of the lateral antennal lobe, which are all innervated by trichoid olfactory receptor neurons. Labeling is seen in both antennal projection and olfactory receptor neurons.

(Ward et al., 2015)

Expression is widespread in the larval ventral nerve cord including the dorsal medial cluster of motor neurons. Expression is also strong in the larval brain lobes but is absent from the optic lobes. Robust expression is seen in motor and sensory axons of the body wall.

(Sanyal, 2009)

Strong reporter expression was observed in the adult brain and weaker expression was observed in the ventral nerve cord, fat body, and oenocytes.

(Legan et al., 2008)

ScerGAL4^D42 is expressed in motor neurons of larvae, and is expressed within thoracic motor neurons and scattered cells throughout the brain of adults.

(Nitz et al., 2002)

In late embryo, expressed in a wide range of tissues.

(Gustafson and Boulianne, 1996)

Marker for

embryonic/larval motor neuron

(Coyne et al., 2014)

motor neuron

(Wittmann et al., 2001)

Reflects expression of

Toll-6

(Ruppert et al., 2017, Ward et al., 2015, Sanyal, 2009, Legan et al., 2008)

Reporter construct used in assay

shi^1.UAS

(Nitz et al., 2002)

kay^UAS.cEa

(Vonhoff et al., 2013)

Jra^UAS.cEa

(Vonhoff et al., 2013)

Scer\GAL80^ChAT.3.3P

(Boerner and Duch, 2010)

Mmus\Cd8a^UAS.cLa.EGFP

(Boerner and Duch, 2010, Sanyal, 2009)

Disc\RFP^UAS.cKa

(Legan et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (4)

Bloomington

8816

w^*; P{GawB}D42

9263

w^*; P{GawB}D42, P{UAS-nSyb-GFP.E}3/TM3, Sb¹

42737

w¹¹¹⁸; P{GawB}D42, P{UAS-mito-HA-GFP.AP}3 e¹/TM6B, Tb¹

606106

w^*; P{GawB}D42, PBac{UAS-CD4-tdGFP}VK00033 P{20XUAS-tdTomato-Sec61β}attP2/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

Insertion 7075bp upstream of the Toll-6 transcription start site (at position 15322725 of scaffold AE014296, genome release r5.13).

(Sanyal, 2009)

The orientation of the insertion was not reported.

(Sanyal, 2009)

Synonyms and Secondary IDs (29)

Reported As

Symbol Synonym

D42 GAL4

(Bakkar et al., 2021, Lehmkuhl et al., 2021, François-Moutal et al., 2019, Maccioni et al., 2018, Faust et al., 2014, Mudher et al., 2004)

D42 Gal4

(Norkett et al., 2020, Bogaert et al., 2018, Coyne et al., 2017, Liu et al., 2017, Choudhury et al., 2016, Chou et al., 2015, Coyne et al., 2015, Coyne et al., 2014, Estes et al., 2013, Chun-Jen Lin et al., 2011, Estes et al., 2011, Wu et al., 2007)

D42 gal4

(Del Castillo et al., 2022)

D42-GAL4

(Parra-Torres et al., 2026, Gbadamosi et al., 2025, Koch and Nagoshi, 2025, Long et al., 2025, Patel et al., 2025, Stavrovskaya et al., 2025, Thi Thu Trinh et al., 2025, Ehweiner et al., 2024, Ikeda et al., 2024, Rankin et al., 2024, Yeewa et al., 2024, Castelli et al., 2023, Han et al., 2023, Kang et al., 2023, Katarachia et al., 2023, Lo Piccolo et al., 2023, Pérez-Moreno et al., 2023, Son et al., 2023, Stubbs et al., 2023, Grice and Liu, 2022, Han et al., 2022, Hernandez-Diaz et al., 2022, Hura et al., 2022, Loganathan et al., 2022, Aragão Gomes et al., 2021, Budnar et al., 2021, Castelli et al., 2021, Coni et al., 2021, Gunaseelan et al., 2021, Kim et al., 2021, Krick et al., 2021, Odnokoz et al., 2021, Park et al., 2021, Balasov et al., 2020, Basargekar et al., 2020, Ibrahim et al., 2020, Kang et al., 2020, Konieczny and Artero, 2020, Singh et al., 2020, Zhang et al., 2020, Guo et al., 2019, Konrad et al., 2019, Lembke et al., 2019, Li et al., 2019, Liu et al., 2019, Lu et al., 2019, Peng et al., 2019, Politano et al., 2019, Fedotov et al., 2018, Heo et al., 2018, Jantrapirom et al., 2018, Mallik et al., 2018, Romano et al., 2018, Steyaert et al., 2018, Straub et al., 2018, Suda et al., 2018, Tran et al., 2018, Yamamoto et al., 2018, Aguilar et al., 2017, Gregory et al., 2017, Hautbergue et al., 2017, Hosaka et al., 2017, Inoshita et al., 2017, Laneve et al., 2017, Lembke et al., 2017, Li et al., 2017, López-Arias et al., 2017, Podratz et al., 2017, Ruppert et al., 2017, Sealey et al., 2017, Wang et al., 2017, Yoon et al., 2017, Baldwin et al., 2016, MacNamee et al., 2016, Cheng et al., 2015, Hagel et al., 2015, Jäckel et al., 2015, Niehues et al., 2015, Petrosyan et al., 2015, Chang et al., 2014, Godena et al., 2014, Kang et al., 2014, Moreau et al., 2014, Moustaqim-Barrette et al., 2014, Titlow et al., 2014, Bahadorani et al., 2013, Borg and Cauchi, 2013, Kuehn and Duch, 2013, Quraishe et al., 2013, Sinadinos et al., 2013, Vanden Broeck et al., 2013, Vonhoff et al., 2013, Zhu et al., 2013, Couthouis et al., 2012, Gregory et al., 2012, Hazelett et al., 2012, Ruiz et al., 2012, Ryglewski et al., 2012, Tsai et al., 2012, Zhou et al., 2012, Couthouis et al., 2011, Freeman et al., 2011, Godena et al., 2011, Lin et al., 2011, Zhong et al., 2011, Bahadorani et al., 2010, Boerner and Duch, 2010, Crocker et al., 2010, Elden et al., 2010, Folwell et al., 2010, Lorenzo et al., 2010, Nedelsky et al., 2010, Owald et al., 2010, Pathak et al., 2010, Radyuk et al., 2010, Shidara and Hollenbeck, 2010, Vonhoff and Duch, 2010, Barber et al., 2009, Dason et al., 2009, Feiguin et al., 2009, Morais et al., 2009, Cauchi et al., 2008, Legan et al., 2008, Lissandron et al., 2007, Llorens et al., 2007, Walker, 2006, Walker et al., 2006, Bronk et al., 2005, Houseley et al., 2005, Orr et al., 2005, Suster et al., 2004, Kuniyoshi et al., 2003, Morey et al., 2003, Chan et al., 2002, Marek and Davis, 2002, Sweeney and Davis, 2002, Wittmann et al., 2001, Shayan and Atwood, 2000, Usui-Aoki et al., 2000, Cheung et al., 1999)

D42-GAl4

(Zhao et al., 2020)

D42-Gal4

(Acheampong et al., 2026, Mallik et al., 2026, Kim and Gelfand, 2025, Klaustermeier et al., 2025, Lu et al., 2025, Mallik et al., 2025, Oh et al., 2025, Waller et al., 2025, Banerjee et al., 2024, Droppelmann et al., 2024, Glineburg et al., 2024, Hendricks and Liebl, 2024, Li et al., 2024, Smith et al., 2024, Tziortzouda et al., 2024, Wang et al., 2024, Bennett et al., 2023, Ervilha Pereira et al., 2023, Mann et al., 2023, Waller and Collins, 2023, Zhao et al., 2023, Cha et al., 2022, Chakravorty et al., 2022, Hanson and Lemaitre, 2022, Inoshita et al., 2022, Molnar et al., 2022, Blázquez-Bernal et al., 2021, Choi et al., 2021, Cowan et al., 2021, Florian et al., 2021, Hengel et al., 2021, Hurley and Staveley, 2021, Insolera et al., 2021, Jarabo et al., 2021, Lund et al., 2021, Balasubramanian and Srinivasan, 2020, Cha et al., 2020, Kiragasi et al., 2020, Martin-Peña and Ferrus, 2020, Montagna et al., 2020, Palazzo et al., 2020, Park et al., 2020, Patel et al., 2020, Pirooznia et al., 2020, Santana et al., 2020, Wang et al., 2020, Yu et al., 2020, Zhang et al., 2020, Donde et al., 2019, Drelon et al., 2019, Espadas et al., 2019, Gong et al., 2019, Hao et al., 2019, Hunt et al., 2019, Imai et al., 2019, Kim et al., 2019, Portela et al., 2019, Vajente et al., 2019, Xiao and Qiu, 2019, Xiao et al., 2019, Zhang et al., 2019, Azpurua et al., 2018, Duncan et al., 2018, Garcia-Lopez et al., 2018, Jonson et al., 2018, Lauwers et al., 2018, Liu et al., 2018, Mattingly et al., 2018, McInnes et al., 2018, Migh et al., 2018, Seong et al., 2018, Xu and Xu, 2018, Alicea et al., 2017, Arnés et al., 2017, Casas-Tintó et al., 2017, Lo Piccolo and Yamaguchi, 2017, Neisch et al., 2017, Raut et al., 2017, Santana and Casas-Tintó, 2017, Vanhauwaert et al., 2017, Yoon et al., 2017, Zhou et al., 2017, Boeynaems et al., 2016, Chao et al., 2016, Chen et al., 2016, Clark et al., 2016, Harel et al., 2016, Mushtaq et al., 2016, Quraishe et al., 2016, Slabbaert et al., 2016, Soukup et al., 2016, Summerville et al., 2016, Beck et al., 2015, Butzlaff et al., 2015, Cagin et al., 2015, Chen et al., 2015, Cowan et al., 2015, Deng et al., 2015, Joardar et al., 2015, Kim et al., 2015, Lu et al., 2015, Pech et al., 2015, Peng et al., 2015, Yang et al., 2015, Chen et al., 2014, Choi et al., 2014, Ehaideb et al., 2014, Harwood et al., 2014, Janssens et al., 2014, Li et al., 2014, Lüchtenborg and Katanaev, 2014, Lüchtenborg et al., 2014, Miskiewicz et al., 2014, Sandoval et al., 2014, Shiraishi et al., 2014, Vasmer et al., 2014, Kern et al., 2013, Kitay et al., 2013, Kottler et al., 2013, Lu et al., 2013, Rahman et al., 2013, Zhan et al., 2013, Bayat et al., 2012, Beck et al., 2012, Füger et al., 2012, Holbrook et al., 2012, Kim et al., 2012, Lee et al., 2012, Pradhan et al., 2012, Rawson et al., 2012, Xia et al., 2012, Estes et al., 2011, Iyengar et al., 2011, Kucherenko et al., 2011, Liu et al., 2011, Rossetto et al., 2011, Stavropoulos and Young, 2011, Yao et al., 2011, Hanson et al., 2010, Khuong et al., 2010, Lone et al., 2010, Melicharek et al., 2010, Nojima et al., 2010, Kissler et al., 2009, Martin et al., 2009, Orso et al., 2009, Chai et al., 2008, Kwak et al., 2008, Huang and Haddad, 2007, Knight et al., 2007, Romero-Pozuelo et al., 2007, Xiao et al., 2007, Grammenoudi et al., 2006, Lind et al., 2006, Schroll et al., 2006, Anderson et al., 2005, Hebbar and Fernandes, 2005, Verstreken et al., 2005, Yoshihara et al., 2005, Landgraf et al., 2003, Missirlis et al., 2003)

D42-gal4

(Chitre et al., 2024, Kour et al., 2023, Kim et al., 2021, Lin et al., 2021, Casci et al., 2019, Pareek et al., 2018, Altanbyek et al., 2016, Mishra-Gorur et al., 2014)

D42.Gal4

(Yang et al., 2024)

D42::Gal4

(Voigt et al., 2010)

D42:Gal4

(Hengel et al., 2025, Even et al., 2021)

D42>GAL4

(Xu et al., 2022)

D42GAL4

(Ulian-Benitez et al., 2017, Elia et al., 1999)

D42Gal4

(Sharma et al., 2025, Dulovic-Mahlow et al., 2019, James et al., 2014, Nechipurenko and Broihier, 2012, James and Broihier, 2011)

GAL4-D42

(Saburova et al., 2017, Cowan et al., 2010, Fischer et al., 2009)

GAL4D42

(De Rose et al., 2017)

Gal4-D42

(Tsakiri et al., 2019, Martin-Pena et al., 2006)

GawB D42

(Wang et al., 2020)

MN-Gal4

(Bosch et al., 2022)

P(GawB)D42

(Haghnia et al., 2007)

P[GawB]D42

(Suda et al., 2018, Pilling et al., 2006)

P[GawB]D42-Gal4

(Horiuchi et al., 2005)

P{GawB}D4

(Schultz et al., 2011)

P{GawB}D42

(Krick et al., 2021, White et al., 2020, Bruels et al., 2019, Trostnikov et al., 2019, Lauwers et al., 2018, Laneve et al., 2017, Zappia and Frolov, 2016, Vanden Broeck et al., 2013, Djagaeva et al., 2012, Barkus et al., 2008, Ford et al., 2007, Kololdziej, 2007.10.30, Miller and Van Vactor, 2005.9, Zheng et al., 2005, FlyBase, 1992-)

Toll-6-GAL4

(McIlroy et al., 2013)

[D42-Gal4]

(Song et al., 2002)

d42-GAL4

(Kishino et al., 2022, Wong et al., 2021, Wong et al., 2014)

d42-Gal4

(Colomb and Brembs, 2016)

d42GAL4

(Strachan et al., 2025, Batlevi et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (403)

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