FlyBase Insertion Report: Dmel\P{GawB}Sxl[NP2426]

General Information

Symbol

Dmel\P{GawB}Sxl^NP2426

Species

D. melanogaster

Name

FlyBase ID

FBti0034845

Feature type

transposable_element_insertion_site

Also Known As

NP2426, Gal4-LN2, LN2-Gal4, NP2426-GAL4, LN2Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, Sxl

Viability / fertility

Associated allele(s)

Scer\GAL4^NP2426, Sxl^NP2426

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

X ( 6F5 )

Sequence Location

X:7,096,129..7,096,129 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:7,096,129..7,096,129 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

6F5 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG216320

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Acebes et al., 2011, Chen et al., 2011, Lieber et al., 2011, Tanaka et al., 2011, Thum et al., 2011, Seki et al., 2010, Das et al., 2008)

Sxl

(FlyBase Curators, 2008-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP2426

Phenotypic Class Phenotype Manifest in Detailed Description

Sxl^NP2426

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- wandering third instar larval stage

GABAergic local interneuron of larval antennal lobe | subset

(Thum et al., 2011)

larval gnathal ganglion

(Thum et al., 2011)

adult stage

adult antennal lobe local neuron | subset

Comment: 37 per antennal lobe

(Acebes et al., 2011, Das et al., 2008)

GABAergic local interneuron of the adult antennal lobe | subset

(Das et al., 2008)

cholinergic local interneuron of the adult antennal lobe | subset

(Das et al., 2008)

adult antennal lobe local neuron lLN2

(Tanaka et al., 2012)

adult local interneuron of the ventral ALv2 neuroblast

(Tanaka et al., 2012)

antennal lobe hub | restricted

(Tanaka et al., 2012)

Additional Information

Statement

Reference

Drives adult expression in around 40 type B local interneurons, including a subset of GABAergic local interneurons of the adult antennal lobe. Drives larval expression in a subset of GABAergic local interneuron of the larval antennal lobe.

(FlyBase Curators, 2017)

ScerGAL4^Sxl-NP2426 drives expression in around 40 type B local interneurons. Their cell bodies cluster in the dorso-lateral region of the antennal lobe and project to all antennal lobe glomeruli and the antennal lobe hub. An additional three interneurons with ventrally located cell bodies are labeled. These project to antennal lobe glomeruli VA5, VA4, VL2a, VM1, VM6, VP1, VP2 and VP3.

(Tanaka et al., 2012)

ScerGAL4^Sxl-NP2426 drives expression in six to eight neurons that innervate the larval antennal lobe (AL) and the subesophageal ganglion. Innervation pattern becomes increasingly dense during larval development. The cell bodies of two to three labeled neurons are located in a cluster ventrolateral to the AL. The other four to five cell bodies are located dorsolateral to the AL.

(Thum et al., 2011)

ScerGAL4^NP2426 is expressed in 37 local neurons of the antennal lobe.

(Acebes et al., 2011)

The approximately 40 somata of cells expressing ScerGAL4^NP2426 are predominantly found in a lateral cell body cluster of the antennal lobe, with a few located in a ventral cell body cluster.

(Das et al., 2008)

Marker for

Reflects expression of

Sxl

(Tanaka et al., 2012, Thum et al., 2011, Das et al., 2008)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Acebes et al., 2011, Das et al., 2008)

Avic\GFP^{Cameleon2.1.UAS}

(Thum et al., 2011)

Scer\GAL80^Mef2.mb247

(Thum et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

104198

y^* w^* P{GawB}Sxl^NP2426 / FM7c

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG216320

Comments

Location X:6941364-6941365 refined by FlyBase alignment of dbGSS accession AG216320 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

GAL4-LN2

(Das et al., 2013, Seki et al., 2010, Okada et al., 2009, Das et al., 2008)

GAL4-NP2426

(Seki et al., 2010)

Gal4-LN2

(Singh et al., 2013, Das et al., 2010)

LN2-GAL4

(Brown et al., 2019, Melnattur et al., 2013)

LN2-Gal4

(Mallick et al., 2024, Casas-Tintó et al., 2017, Acebes et al., 2011)

LN2.GAL4

(Lieber et al., 2011)

LN2GAL4

(Hamid et al., 2021)

LN2Gal4

(Kanellopoulos et al., 2020)

NP2426

(Hamid et al., 2021, Brown et al., 2019, Martelli and Fiala, 2019, Scholz-Kornehl and Schwärzel, 2016, Kidd et al., 2015, Tanaka et al., 2012, Acebes et al., 2011, Thum et al., 2011, Okada et al., 2009, Das et al., 2008)

NP2426-GAL4

(Franco et al., 2017, Tanaka et al., 2011)

NP2426-Gal4

(Mallick et al., 2024, Kanellopoulos et al., 2020, Casas-Tintó et al., 2017)

Np2426

(Chen et al., 2011)

P{GawB}NP2426

(FlyBase, 1992-)

P{GawB}NP2426-5-1

(FlyBase, 2005, FlyBase, 1992-)

P{GawB}Sxl^NP2426

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

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