FlyBase Insertion Report: Dmel\P{GawB}NP2631

General Information

Symbol

Dmel\P{GawB}NP2631

Species

D. melanogaster

Name

FlyBase ID

FBti0034959

Feature type

transposable_element_insertion_site

Also Known As

NP2631-gal4, NP2631, optix-Gal4, OptixGAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP2631

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 44A3 )

Sequence Location

2R:8,029,918..8,029,979 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:8,029,918..8,029,979 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

44A3 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG216420

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Nfonsam et al., 2012, Pitman et al., 2011, Pauls et al., 2010, Yu et al., 2010, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP2631

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- late third instar larval stage

Optix-expressing compartment of the outer optic anlage

(Gold and Brand, 2014)

adult stage

adult mushroom body anterior paired lateral cell

(Flybrain Neuron Database et al., 2018.6.28, Pitman et al., 2011, Tanaka et al., 2008)

adult protocerebrum | subset

(Tanaka et al., 2008)

adult mushroom body alpha-lobe

(Tanaka et al., 2008)

adult mushroom body beta-lobe

(Tanaka et al., 2008)

pedunculus of adult mushroom body

(Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult mushroom body dorsal accessory calyx

(Tanaka et al., 2008)

neuron of adult median bundle

(Pitman et al., 2011)

neuron of protocerebral bridge

(Pitman et al., 2011)

subesophageal zone neuron

(Pitman et al., 2011)

adult mushroom body gamma-lobe

(Tanaka et al., 2008)

adult mushroom body beta'-lobe

(Tanaka et al., 2008)

adult mushroom body alpha'-lobe

(Tanaka et al., 2008)

adult fruitless P1 (male) neuron

(von Philipsborn et al., 2014)

cell body rind of adult medulla

(Gold and Brand, 2014)

lamina | restricted

(Gold and Brand, 2014)

postfrons | medial

(Domínguez-Cejudo and Casares, 2015)

adult head capsule | restricted

(Domínguez-Cejudo and Casares, 2015)

adult stage | male

adult fruitless P1 (male) neuron

(von Philipsborn et al., 2011)

fruitless expressing neuron | subset

(von Philipsborn et al., 2011)

Additional Information

Statement

Reference

Drives expression in adult mushroom body anterior paired lateral cell (APL), and in the Optix-expressing compartment of the outer optic anlage at larval stages.

(FlyBase Curators, 2017)

ScerGAL4^NP2631 drives expression in the dorsal eye disc in a pattern recapitulating that of Optix. In the adult head, ScerGAL4^NP2631 drives expression in a medial stripe running from the anterior edge of the dorsal head capsule to the anterior ocellus. ScerGAL4^NP2631 does not drive expression in the embryonic primordium of the eye-antennal disc.

(Domínguez-Cejudo and Casares, 2015)

ScerGAL4^NP2631 drives expression that overlaps with Optix. In the adult, it is expressed in much of the cell body rind of medulla and part of the lamina with a clear central gap that corresponds to anterior boundaries seen in larval Optix expression.

(Gold and Brand, 2014)

ScerGAL4^NP2631 is expressed in a subset of neurons in the adult brain, including the median bundle, protocerebral bridge, subesophageal ganglion and the mushroom body anterior paired lateral cell.

(Pitman et al., 2011)

Marker for

adult fruitless P1 (male) neuron

(von Philipsborn et al., 2014)

Reflects expression of

Optix

(Domínguez-Cejudo and Casares, 2015, Gold and Brand, 2014)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Pitman et al., 2011, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Mmus\Cd8a^{FRT.UAS.cYa.GFP}

(von Philipsborn et al., 2014, von Philipsborn et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

104266

w^*; P{GawB}NP2631 / CyO

External Crossreferences and Linkouts ( 0 )

Comments

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0239559 (see personal communication to FlyBase FBrf0241237).

(Ribeiro et al., 2018)

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0225494 (see personal communication to FlyBase FBrf0241237).

(Bath et al., 2014)

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0212984 (see personal communication to FlyBase FBrf0241237).

(von Philipsborn et al., 2011)

Location 2R:3541729-3541792 determined by FlyBase alignment of dbGSS accession AG216420 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

NP2631

(Li et al., 2026, Wang et al., 2021, Ishii et al., 2020, Wohl et al., 2020, Dickson, 2019.1.23, Flybrain Neuron Database et al., 2018.6.28, Richard et al., 2017, Scholz-Kornehl and Schwärzel, 2016, Gold and Brand, 2014, von Philipsborn et al., 2014, Pitman et al., 2011, Pauls et al., 2010, Yu et al., 2010, Tanaka et al., 2008)

NP2631-GAL4

(Mancini et al., 2023, Mohamed et al., 2023, Wong et al., 2021, Amin et al., 2020, Sato et al., 2019, Drago and Davis, 2016, Haynes et al., 2015, Lin et al., 2014)

NP2631-Gal4

(Mayseless et al., 2023, Chiu et al., 2021, Pacheco et al., 2021, Bielopolski et al., 2019, Zhou et al., 2019, Mayseless et al., 2018, Rist and Thum, 2017)

NP2631-gal4

(Cervantes-Sandoval et al., 2016)

NP2631.GAL4

(O'Sullivan et al., 2018)

Optix-GAL4

(Malin et al., 2024)

Optix-Gal4

(Courgeon and Desplan, 2019)

Optix-Gal4^NP2631

(Courgeon and Desplan, 2019)

OptixGAL4

(Gold and Brand, 2014)

OptixNP2631-GAL4

(Domínguez-Cejudo and Casares, 2015)

P1-GAL4

(Ribeiro et al., 2018)

P{GawB}NP2631

(FlyBase, 1992-)

P{GawB}NP2631-5-1

(FlyBase, 2005, FlyBase, 1992-)

np2631-Gal4

(Nfonsam et al., 2012)

optix-Gal4

(Wang et al., 2021, Shard et al., 2020)

optix-gal4

(Hirose-Shimizu et al., 2026)

optixGal4

(Richard et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (45)

Report Sections

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