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General Information
Symbol
Dmel\P{GawB}NP2631
Species
D. melanogaster
Name
FlyBase ID
FBti0034959
Also Known As
NP2631-gal4, NP2631, optix-Gal4, OptixGAL4
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2R ( 44A3 )
Sequence Location
2R:8,029,918..8,029,979 [+]
Target / Docking site
Member of Large Scale Dataset(s)
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:8,029,918..8,029,979 [+]
Orientation
Cytological location (computed by FlyBase)
44A3 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in adult mushroom body anterior paired lateral cell (APL), and in the Optix-expressing compartment of the outer optic anlage at larval stages.

Scer\GAL4NP2631 drives expression in the dorsal eye disc in a pattern recapitulating that of Optix. In the adult head, Scer\GAL4NP2631 drives expression in a medial stripe running from the anterior edge of the dorsal head capsule to the anterior ocellus. Scer\GAL4NP2631 does not drive expression in the embryonic primordium of the eye-antennal disc.

Scer\GAL4NP2631 drives expression that overlaps with Optix. In the adult, it is expressed in much of the cell body rind of medulla and part of the lamina with a clear central gap that corresponds to anterior boundaries seen in larval Optix expression.

Scer\GAL4NP2631 is expressed in a subset of neurons in the adult brain, including the median bundle, protocerebral bridge, subesophageal ganglion and the mushroom body anterior paired lateral cell.

 
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0239559 (see personal communication to FlyBase FBrf0241237).

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0225494 (see personal communication to FlyBase FBrf0241237).

FlyBase curator comment: the 'NP2631' GAL4 driver line was incorrectly described as 'NP2361' in FBrf0212984 (see personal communication to FlyBase FBrf0241237).

Location 2R:3541729-3541792 determined by FlyBase alignment of dbGSS accession AG216420 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

Synonyms and Secondary IDs (15)
References (37)