FlyBase Insertion Report: Dmel\TI{lexA::VP16}fru[P1.LexA]

General Information

Symbol

Dmel\TI{lexA::VP16}fru^P1.LexA

Species

D. melanogaster

Name

FlyBase ID

FBti0168676

Feature type

insertion_site

Also Known As

fru-LexA, Fru^LexA, FruP1-LexA, FruLexA

Description

Inserted Element

TI{lexA::VP16}

LINE ID

Affected gene(s)

Ecol\lexA, fru

Viability / fertility

Associated allele(s)

Ecol\lexA^fru.P1, fru^P1.LexA

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 91A6-91B3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

91A6-91B3 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{lexA::VP16}

(Mellert et al., 2010)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lexA

(Vaughan et al., 2014)

fru

(Chen et al., 2021, Sun et al., 2020, Wang et al., 2020, Wohl et al., 2020, Wu et al., 2019, Machado et al., 2017, Kallman et al., 2015, Zhou et al., 2014, Mellert et al., 2010)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lexA^fru.P1

Phenotypic Class Phenotype Manifest in Detailed Description

fru^P1.LexA

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- adult stage

gustatory receptor neuron | subset of prothoracic tarsal segment

(Mellert et al., 2010)

adult stage

prothoracic leg taste bristle chemosensory neuron | subset

(Lu et al., 2012)

adult brain | restricted

(Li et al., 2011)

adult mushroom body alpha-lobe | restricted

(Li et al., 2011)

adult mushroom body beta-lobe | restricted

(Li et al., 2011)

adult mushroom body gamma-lobe | restricted

(Li et al., 2011)

adult mushroom body alpha'-lobe | restricted

(Li et al., 2011)

adult mushroom body beta'-lobe | restricted

(Li et al., 2011)

antennal lobe glomerulus DA1

(Li et al., 2011)

antennal lobe glomerulus VA1v

(Li et al., 2011)

fruitless expressing neuron

(Wang et al., 2020, Meissner et al., 2016)

fruitless-expressing lobula columnar neuron LC10a

(Ribeiro et al., 2018)

adult doublesex pCd (male) neuron | subset

(Jung et al., 2020)

adult Drosulfakinin MP1 neuron | subset

Comment: co-localizes with P{Dsk-GAL4.W}.

A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with P{Dsk-GAL4.W}. Only stochastic expression observed.

(Wu et al., 2019)

adult Drosulfakinin MP3 neuron | subset

Comment: co-localizes with P{Dsk-GAL4.W}.

A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with P{Dsk-GAL4.W}. Only stochastic expression observed.

(Wu et al., 2019)

adult fruitless P1 (male) neuron

Comment: A FLP/FRT strategy shows TI{lexA::VP16}fru^P1.LexA drives expression in adult ingestion neuron 1 when combined with TI{GAL4::p65}CCKLR-17D3^GAL4::p65.

(Wu et al., 2019)

oviposition descending neuron oviDN

(Wang et al., 2020)

labellar taste bristle mechanosensory neuron | subset

(Thistle et al., 2012)

leg taste bristle chemosensory neuron | subset

(Thistle et al., 2012)

adult fruitless pIP10 (male) neuron

(Lillvis et al., 2024)

adult fruitless pMP2 neuron

(Lillvis et al., 2024)

adult fruitless dPR1 neuron

(Lillvis et al., 2024)

adult fruitless dMS9 neuron

(Lillvis et al., 2024)

adult fruitless vMS12 neuron

(Lillvis et al., 2024)

adult doublesex TN1A (male) neuron

(Lillvis et al., 2024)

adult fruitless dMS2 neuron

(Lillvis et al., 2024)

adult fruitless vPR9 neuron

(Lillvis et al., 2024)

adult fruitless dMS5 neuron

(Lillvis et al., 2024)

adult fruitless dMS10 neuron

(Lillvis et al., 2024)

adult doublesex TN1C (male) neuron

(Lillvis et al., 2024)

adult fruitless vMS13 neuron

(Lillvis et al., 2024)

adult fruitless vMS16 neuron

(Lillvis et al., 2024)

adult fruitless vMS17 neuron

(Lillvis et al., 2024)

adult fruitless vPR1 neuron

(Lillvis et al., 2024)

adult fruitless vPR6 (male) neuron

(Lillvis et al., 2024)

adult fruitless vPR13 neuron

(Lillvis et al., 2024)

descending neuron of the posterior brain DNp13 (male)

(Lillvis et al., 2024)

adult doublesex TN1B (male) neuron

(Lillvis et al., 2024)

adult doublesex TN1D (male) neuron

(Lillvis et al., 2024)

adult doublesex TN1E (male) neuron

(Lillvis et al., 2024)

adult fruitless vMS11 neuron

(Lillvis et al., 2024)

adult fruitless vPR7 neuron

(Lillvis et al., 2024)

descending neuron of the posterior brain DNp36 (male)

(Lillvis et al., 2024)

adult stage | female

neuron | subset of lateral oviduct

Comment: 1 neuron

(Rezaval et al., 2012)

neuron | subset of uterus

Comment: 3 neurons

(Rezaval et al., 2012)

adult post-embryonic ventral posterior Ilp7 (female) neuron | subset

(Feng et al., 2014)

adult stage | male

adult doublesex pC1 (male) neuron | subset

(Zhou et al., 2014)

adult doublesex pC2 (male) neuron | subset

(Zhou et al., 2014)

adult doublesex pCd (male) neuron | subset

(Zhou et al., 2014)

Additional Information

Statement

Reference

Drives expression in most fruitless expressing neurons in the adult, including neurons of the male and female reproductive systems, and a small subset of gustatory receptor neurons.

(FlyBase Curators, 2017)

Ecol\lexA^fru.P1 drives expression in mechanosensory neurons in the taste bristles of the proboscis. In the legs, Ecol\lexA^fru.P1 is detected in more then one gustatory receptor neuron per bristle, with these cells also expressing Scer\GAL4^ppk23.2.695. Ecol\lexA^fru.P1 positive neurons do not overlap with expression of Scer\GAL4^Gr64f.9.7 which labels the sugar-responsive neurons, candidate pheromone receptors Scer\GAL4^Gr32a.3.776 and Scer\GAL4^Gr68a.PB, or with Scer\GAL4^Gr66a.3.153 which labels bitter-responsive neurons.

(Thistle et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in chemosensory neurons in the male forelegs. Most of these chemosensory neurons labeled with EcollexA^{fru.P1.T:HsimVP16} also express ScerGAL4^ppk23.2.6.

(Lu et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in mechanosensory neurons in the taste bristles of the proboscis. In the legs, EcollexA^{fru.P1.T:HsimVP16} is detected in more then one gustatory receptor neuron per bristle, with these cells also expressing ScerGAL4^ppk23.2.695. EcollexA^{fru.P1.T:HsimVP16} positive neurons do not overlap with expression of ScerGAL4^Gr64f.9.7 which labels the sugar-responsive neurons, candidate pheromone receptors ScerGAL4^Gr32a.3.776 and ScerGAL4^Gr68a.PB, or with ScerGAL4^Gr66a.3.153 which labels bitter-responsive neurons.

(Thistle et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in 2 clusters of neurons in the adult female genital tract, which co-express AvicGFP^1.E.ppk : one cluster of 1 neuron in the lateral oviduct and one cluster of 3 neurons in the uterus (which also express ScerGAL4^dsx.KI).

(Rezaval et al., 2012)

EcollexA^{fru.P1.T:HsimVP16} drives expression in many neurons in the adult brain, including in the mushroom body alpha, beta, gamma, alpha', beta' lobes. In the antennal lobe, EcollexA^{fru.P1.T:HsimVP16} labels the antennal lobe glomeruli DA1 and VA1v.

(Li et al., 2011)

EcollexA^fru.P1 drives expression in in a subset of gustatory neurons of prothoracic tarsal segments 3-5 at 18-20 hours APF; no expression is observed prothoracic tarsal segments 1-2 at this stage. By 24-28 hours, expression is driven in gustatory neurons of all prothoracic tarsal segments. At the earlier timepoint, expression is observed in both male and female prothoracic tarsi, while expression at 28 hours APF is much more pronounced in male than female tarsi.

(Mellert et al., 2010)

Marker for

fruitless expressing neuron

(Wang et al., 2020)

Reflects expression of

fru

(Wang et al., 2020, Wang et al., 2020, Ribeiro et al., 2018, Meissner et al., 2016, Zhou et al., 2014, Lu et al., 2012, Rezaval et al., 2012, Thistle et al., 2012, Li et al., 2011, Mellert et al., 2010)

Reporter construct used in assay

Disc\RFP^{tdTomato.lexAop.Tag:NLS(tra)}

(Rezaval et al., 2012)

Rnor\Cd2^lexAop.GFP

(Feng et al., 2014, Lu et al., 2012, Thistle et al., 2012, Mellert et al., 2010)

Disc\RFP^{mCherry.lexAop.Tag:Myr(Unk)}

(Li et al., 2011)

Avic\GFP^{2xhrGFP.lexAop.Tag:NLS(SV40-largeT)}

(Li et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

66698

w^*; wg^Sp-1/CyO; TI{lexA::VP16}fru^P1.LexA/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Fru-LexA

(Zhang et al., 2022, Deutsch et al., 2020, Jung et al., 2020, Zhang et al., 2019)

Fru^LexA

(Clowney et al., 2015)

FruLexA

(Mezzera et al., 2020)

FruP1-LexA

(Brovkina et al., 2021)

TI{lexA::VP16}fru^P1.LexA

(FlyBase, 1992-)

fru-LexA

(Yamanouchi et al., 2023, Wang et al., 2021, Wang et al., 2020, Wang et al., 2020, He et al., 2019, Shao et al., 2019, Machado et al., 2017, Harris et al., 2015, Kallman et al., 2015)

fru-lexA

(Wei et al., 2025, Lee et al., 2023)

fru^LexA

(Ma et al., 2022, Arez et al., 2021, Seeholzer et al., 2018)

fru^P1-LexA

(Arez et al., 2021)

fru^P1.LexA

(Watanabe et al., 2017)

fruLexA

(Liu et al., 2024)

fruP1-LexA

(Kimura et al., 2024, Vernier et al., 2023)

fruP1.LexA

(Lillvis et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (43)

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