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FB2021_02, released April 13, 2021

Recombinant Construct: P{UAS-GFP.nls}

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General Information
Symbol
P{UAS-GFP.nls}
FlyBase ID
FBtp0001204
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPUAS.Tag:NLS(SV40-largeT)
Also Known As
UAS-GFP.nls, UAS-GFP, UAS-GFPnls, UAS-nls-GFP, UAS-nlsGFP, UAS-GFPnls, UAS-nGFP, UAS-GFP-NLS, GFP-NLS, UAS-nls::GFP
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Shiga et al., 1996)
Construct components
Product class / Tool use(s)
Marker(s)
w+mC
Component allele Avic\GFPUAS.Tag:NLS(SV40-largeT)
Product class / Tool use(s)
Regulatory region(s)
UAS
Encoded product / tool
GFP
Tagged with
Tag:NLS(SV40-largeT)
Description

Expression of GFP, tagged at its N terminal end with the 15 amino acid SV40 nuclear localization signal, is governed by UAS regulatory sequences.

(Shiga et al., 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
(Shiga et al., 1996)
chimeric
gene
(Shiga et al., 1996)
molecular
tag
(Shiga et al., 1996)
reporter
gene
(Shiga et al., 1996)
selectable
marker
(Shiga et al., 1996)
Component Alleles
Allele
Avic\GFPUAS.Tag:NLS(SV40-largeT)
Reference(s)
(Ariyapala et al., 2020, Mehta et al., 2020, Ogienko et al., 2020, Rambur et al., 2020, Weaver et al., 2020, Andreyeva et al., 2019, Guo et al., 2019, Morón-Oset et al., 2019, Phan et al., 2019, Conway et al., 2018, Pu et al., 2018, Perry et al., 2015, Azuma et al., 2014, Harrop et al., 2014, Hauling et al., 2014, Lawton et al., 2014, Martín-Peña et al., 2014, Eun et al., 2013, Hopkins, 2013, Ibrahim et al., 2013, Jepson et al., 2013, Lindström et al., 2013, Neisch et al., 2013, Shen et al., 2013, Uchino et al., 2013, Dahlgaard et al., 2012, Denton et al., 2012, Edwards et al., 2012, Kennell et al., 2012, Saleem et al., 2012, Unhavaithaya and Orr-Weaver, 2012, Weiss et al., 2012, Acebes et al., 2011, Benchabane et al., 2011, Benchabane et al., 2011, Cáceres et al., 2011, Cho and Fischer, 2011, Estes et al., 2011, Gallio et al., 2011, Hasegawa et al., 2011, Keller et al., 2011, Lee et al., 2011, Li et al., 2011, Vrailas-Mortimer et al., 2011, Wang et al., 2011, Alekseyenko et al., 2010, Ballard et al., 2010, Lam et al., 2010, Zhang et al., 2010, Guha et al., 2009, Issigonis et al., 2009, Jacobsson et al., 2009, Jansen et al., 2009, Mitri et al., 2009, Mohseni et al., 2009, Mosimann et al., 2009, Shahidullah et al., 2009, Awasaki et al., 2008, Chang et al., 2008, Chen et al., 2008, Costantino et al., 2008, Hong et al., 2008, Lee et al., 2008, Thorne and Amrein, 2008, Watson et al., 2008, Wu et al., 2008, Caldwell et al., 2007, Duan et al., 2007, Kiger et al., 2007, Wada et al., 2007, Wang et al., 2007, Wang et al., 2007, Baena-Lopez and Garcia-Bellido, 2006, Le Bras and Van Doren, 2006, Maqbool et al., 2006, Mosimann et al., 2006, Muro et al., 2006, Palanker et al., 2006, Sato et al., 2006, Umetsu et al., 2006, Yasunaga et al., 2006, Araki et al., 2005, Caldwell et al., 2005, Caldwell et al., 2005, Chen et al., 2005, Cole et al., 2005, Guha and Kornberg, 2005, McClure and Schubiger, 2005, Monier et al., 2005, Ralston and Blair, 2005, Rawson, 2005, Rawson et al., 2005, Xu et al., 2005, Yoshida et al., 2005, Yuan et al., 2005, Dominguez et al., 2004, Dutta et al., 2004, Galko and Krasnow, 2004, Kimura et al., 2004, Soler et al., 2004, Thorne et al., 2004, Araki et al., 2003, Bidet et al., 2003, Cherbas et al., 2003, Fichelson and Gho, 2003, Kenyon et al., 2003, Labrador and Corces, 2003, Marygold and Vincent, 2003, Mergliano and Minden, 2003, Perrin et al., 2003, Robertson et al., 2003, Roignant et al., 2003, Sen et al., 2003, Ahmad and Baker, 2002, Behan et al., 2002, Held, 2002, Rulifson et al., 2002, Williams and Shepherd, 2002, Dong et al., 2001, Keisman et al., 2001, Loren et al., 2001, Pfeiffer et al., 2000, Gho et al., 1999, Jarecki et al., 1999, Weinkove et al., 1999, Shiga et al., 1996)
Molecular data

Expression of GFP, tagged at its N terminal end with the 15 amino acid SV40 nuclear localization signal, is governed by UAS regulatory sequences.

(Shiga et al., 1996)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{UAS-GFP.nls}
(Shiga et al., 1996)
descendant(s)
Comments

report comment: Sequences for SV40 nuclear localization signal fused at 5\' end of GFP sequences.

(Shiga et al., 1996)

construct_comment: Designed as vital cell marker, however levels of fluorescence very low in diploid cells.

(Shiga et al., 1996)
Stocks (6)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (52)
Reported As
Symbol Synonym
GFP-NLS
(Estes et al., 2011, Yuan et al., 2005)
GFP-lacZ
(Benchabane et al., 2011)
GFP-nls
(Weiss et al., 2012)
GFP.nls
(Andreyeva et al., 2019, Costantino et al., 2008)
GFPnls
(Martín-Peña et al., 2014)
GFPN
(FlyBase, 1996-)
GFPnls
(Hopkins, 2013)
NLS:GFP
(Gallio et al., 2011)
P {w [+mC]= UAS-GFP. nls}
(Azuma et al., 2014)
PUAS-GFP.nls
(Wang et al., 2007)
Pw<up>+;UAS-GFP nls</up>
(Rawson, 2005)
P{UAS-GFP.N}
(FlyBase, 1996-)
P{UAS-GFP.nls}
(Conway et al., 2018, Harrop et al., 2014, Hopkins, 2013, Edwards et al., 2012, Li et al., 2011, Jacobsson et al., 2009, Mohseni et al., 2009, Watson et al., 2008, FlyBase, 1996-)
UAS-EGFPnls
(Cáceres et al., 2011)
UAS-GFP
(Ogienko et al., 2020, Azuma et al., 2014, Eun et al., 2013, Ibrahim et al., 2013, Kennell et al., 2012, Vrailas-Mortimer et al., 2011, Lam et al., 2010, Issigonis et al., 2009, Shahidullah et al., 2009, Muro et al., 2006, Ralston and Blair, 2005, Xu et al., 2005, Roignant et al., 2003)
UAS-GFP(nls)
(Caldwell et al., 2005, Caldwell et al., 2005)
UAS-GFP-NLS
(Shen et al., 2013, Araki et al., 2005, Araki et al., 2003)
UAS-GFP-lacZ
(Benchabane et al., 2011)
UAS-GFP-nls
(Saleem et al., 2012, Chen et al., 2008)
UAS-GFP-nls14
(Kenyon et al., 2003)
UAS-GFP.nls
(Ariyapala et al., 2020, Rambur et al., 2020, Weaver et al., 2020, Hauling et al., 2014, Lindström et al., 2013, Acebes et al., 2011, Jansen et al., 2009, Costantino et al., 2008, Kiger et al., 2007, Wang et al., 2007, Cole et al., 2005, Galko and Krasnow, 2004, Bidet et al., 2003, Cherbas et al., 2003, Ahmad and Baker, 2002, Keisman et al., 2001)
UAS-GFP.nls-14
(Le Bras and Van Doren, 2006)
UAS-GFP::nls
(Issigonis et al., 2009)
UAS-GFPNLS
(McClure and Schubiger, 2005)
UAS-GFPNLS
(Neisch et al., 2013, Mosimann et al., 2009, Mosimann et al., 2006)
UAS-GFPnls.14
(Marygold and Vincent, 2003)
UAS-GFPnls
(Phan et al., 2019, Unhavaithaya and Orr-Weaver, 2012, Li et al., 2011, Mohseni et al., 2009, Dominguez et al., 2004)
UAS-GFPN
(Kimura et al., 2004)
UAS-GFPnls
(Uchino et al., 2013, Dahlgaard et al., 2012, Kennell et al., 2012, Duan et al., 2007, Baena-Lopez and Garcia-Bellido, 2006, Maqbool et al., 2006, Yasunaga et al., 2006, Yoshida et al., 2005, Soler et al., 2004, Mergliano and Minden, 2003)
UAS-eGFP.NLS
(Morón-Oset et al., 2019)
UAS-gfp-nls
(Jepson et al., 2013)
UAS-gfp.nls
(Wada et al., 2007)
UAS-gfpn
(Shiga et al., 1996)
UAS-gfpnlacZ
(Chihara and Hayashi, 2000, Ikeya and Hayashi, 1999)
UAS-nGFP
(Ballard et al., 2010, Lee et al., 2008, Palanker et al., 2006, Mergliano and Minden, 2003, Robertson et al., 2003, Jarecki et al., 1999)
UAS-ngfp
(Cho and Fischer, 2011, Zhang et al., 2010)
UAS-nls GFP
(Guha and Kornberg, 2005)
UAS-nls-EGFP
(Allan et al., 2003)
UAS-nls-GFP
(Pu et al., 2018, Chang et al., 2008, Caldwell et al., 2007, Monier et al., 2005, Dutta et al., 2004, Fichelson and Gho, 2003, Held, 2002, Dong et al., 2001, Gho et al., 1999)
UAS-nls::GFP
(Guo et al., 2019, Lawton et al., 2014, Lee et al., 2011)
UAS-nls:GFP
(Alekseyenko et al., 2010)
UAS-nlsGFP
(Denton et al., 2012, Hasegawa et al., 2011, Mitri et al., 2009, Awasaki et al., 2008, Hong et al., 2008, Sato et al., 2006, Umetsu et al., 2006, Williams and Shepherd, 2002, Pfeiffer et al., 2000)
UAS-nucGFP
(Thorne and Amrein, 2008, Thorne et al., 2004)
UAS-nucgfp8
(Wu et al., 2008)
UAS-nuclear GFP
(Rulifson et al., 2002)
UAS-nuclear-localization-signal-GFP
(Keller et al., 2011)
UAS-nuclearGFP
(Loren et al., 2001)
UASGFP
(Perrin et al., 2003)
UGPnls
(Robertson et al., 2003)
nls GFP
(Guha et al., 2009)
uas-GFP
(Wang et al., 2011)
{UAS-GFP.nls}
(Costantino et al., 2008)
Secondary FlyBase IDs
    References (130)