FB2025_01 , released February 20, 2025
Recombinant Construct: P{walLy}
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General Information
Symbol
P{walLy}
FlyBase ID
FBtp0001569
Feature type
transgenic_transposable_element
Component Allele(s)
walter
y+t7.7
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
y+t7.7
Component allele walter
Transgenic product class
Component
Use(s)
Encoded product / tool
w
Description

Construct: 2456bp of contiguous w locus sequence on one side of the site of P-element excision in "whd" allele series and 999bp on the other.

(Engels et al., 1994)

Construct: P{walLKP} contains an insertion of a 1124bp KP element (missing the first and last 15 nucleotides) compared to P{walL}. Construct: P{walL} contains an insertion of a polylinker at position -2368 (numbering scheme from FBrf0041384) compared to P{walter}. Construct: P{walLy} contains an insertion of y+t7.7 into the polylinker of P{walL}. 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence.

(Nassif et al., 1994)

12 substitutions of single base pairs within a region of approximately 3kb of a w minigene.

(Lankenau, 1995)

Construct: P{walter} contains 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence. Construct: P{walLf} contains an insertion of an approximately 13,000bp f gene fragment (f+t13) into the polylinker of P{walL}. Construct: P{walLG} contains an insertion of approximately 4000bp DNA into the polylinker of P{walL}. Construct: P{walLK} contains an insertion of 1858bp DNA into the polylinker of P{walL}.

(Keeler et al., 1996)

Contains a w gene with 12 single-base alterations compared to the canonical w sequence together with y gene sequences.

(Keeler and Gloor, 1997)
Component allele y+t7.7
Transgenic product class
genomic_DNA
(Geyer and Corces, 1987)
wild_type
(Geyer and Corces, 1987)
Component
Use(s)
Regulatory region(s)
y
y_D-2873
Encoded product / tool
y
Description

8kb SalI fragment carrying y+.

(Merriam, 1997.5.9)

Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences.

(Geyer and Corces, 1987)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: One of a series of constructs used to characterize mechanism of P-element transposition and repair of double-strand DNA breaks.

(Nassif et al., 1994)

report comment: walL carries multiple single base changes which add or remove restriction sites, relative to wild-type w. Allows determination of origin of tracks of DNA after double strand break and repair.

(Nassif et al., 1994)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{walLy}
(Coveny et al., 2002, FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)