FB2025_04 , released October 2, 2025
Recombinant Construct: P{GAL4-Act5C(FRT.CD2).P}
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General Information
Symbol
P{GAL4-Act5C(FRT.CD2).P}
FlyBase ID
FBtp0001640
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4FRT.Rnor\Cd2.Act5C
Also Known As
Act>CD2>Gal4, Act5C>CD2>Gal4, actin>CD2>Gal4, Actin5C>CD2>Gal4, act > cd2 > GAL4, act<CD2<GAL4, act<FRT-CD2-FRT<Gal4, actin-FRT-Stop-FRT-Gal4, act>CD2stop>Gal4, actP>CD2>Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
4 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4FRT.Rnor\Cd2.Act5C
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
GAL4
Also carries
FRT
Description

Act5C promoter is fused upstream of an FRT cassette carrying Rnor\Cd2 sequences, which in turn is fused upstream of sequence encoding a GAL4 driver.

(Pignoni and Zipursky, 1997)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference

Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment carrying RnorCd2. Expression is of ScerGAL4Act5C.PP, carried in descendant transposon P{GAL4-Act5C(-FRT).P}; see ScerGAL4Act5C.PP elsewhere in this table.

(FlyBase Curators, 2017)

Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment carrying RnorCd2. See expression pattern for ScerGAL4Act5C.PP, carried in descendant transposon P{GAL4-Act5C(-FRT).P}.

(Pignoni and Zipursky, 1997)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: FRT-deleted (active) form of Act5C-GAL4 carried on descendant transposon P{GAL4-Act5C(-FRT).P}.

(Pignoni and Zipursky, 1997)

report comment:

(Pignoni and Zipursky, 1997)
Stocks (9)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (157)
Reported As
Symbol Synonym
5c>CD2>Gal4
(Datar et al., 2000)
A>CD2>GAL4
(Lyulcheva et al., 2008)
Act > CD2 > GAL4
(Shen et al., 2021, Fernando et al., 2015, Kis et al., 2015)
Act > CD2 > Gal4
(Ma et al., 2023, Lakatos et al., 2019, Érdi et al., 2012)
Act >> GAL4
(Giráldez et al., 2002)
Act(FRT.stop)GAL4
(Heinrich and Ryglewski, 2020)
Act-FRT-CD2-FRTgal4
(Mishra-Gorur et al., 2014)
Act-FRT-STOP-FRT-GAL4
(Xie et al., 2021)
Act-FRT-Stop-FRT-GAL4
(Xiao et al., 2012)
Act-FRT-stop-FRT-lacZ(nls)
(Janssens et al., 2014)
Act-gal4 (FRT.CD2 STOP)
(Parreno et al., 2024)
Act.CD2.Gal4
(Pircs et al., 2012)
Act.FRT-CD2-FRT.GAL4
(Liu et al., 2025)
Act.FRT-CD2-FRT.Gal4
(Du et al., 2020)
Act5C > CD2 > GAL4
(Swaminathan and Pile, 2010)
Act5C > CD2 > Gal4
(Romani et al., 2016)
Act5C > CD8 > GAL4
(Gavory et al., 2021)
Act5C promoter-FRT-CD2-FRT-GAL4
(Galant et al., 2002)
Act5C(FRT.CD2)-Gal4
(Dong et al., 2020, Dong et al., 2015)
Act5C(FRT.CD2)Gal4
(Galagovsky et al., 2018)
Act5C.CD2.GAL4
(Singari et al., 2014)
Act5C.CD2.Gal4
(Ashton-Beaucage et al., 2016)
Act5C<CD2<GAL4
(Szuplewski et al., 2003)
Act5C<CD2>GAL4
(Simcox et al., 2008)
Act5C<FRT>CD2<FRT>GAL4
(Rulifson et al., 2000)
Act5C>>CD2>>Gal4
(Roignant et al., 2003)
Act5C>CD2>GAL4
(Madan et al., 2024, Swaminathan and Pile, 2010, Schlichting and Dahmann, 2008, Schlichting et al., 2006, Hennig and Neufeld, 2002, Kopp and Duncan, 2002, Tseng and Hariharan, 2002)
Act5C>CD2>Gal4
(Sui and Dahmann, 2024, Cheng et al., 2021, Fic et al., 2021, Gunawan et al., 2013, Demontis and Dahmann, 2009, Bernardi et al., 2007, Hyun et al., 2006, Huh et al., 2004, Jhaveri et al., 2004, Emerald et al., 2003, Sen et al., 2003, Tapon et al., 2001, Denef et al., 2000, Jhaveri et al., 2000, Gibson and Schubiger, 1999)
Act5C>FRT.CD2>GAL4
(Worley et al., 2018)
Act5C>STOP>GAL4
(Lavalou et al., 2021)
Act5C>stop>Gal4
(Perea et al., 2013)
Act5c(FRT-CD2)-Gal4
(Mika et al., 2021)
Act5c(FRT.CD2)Gal4
(Sullivan et al., 2019)
Act5c-FRT-CD2-FRT-Gal4
(Pahl et al., 2019)
Act5c>CD2>GAL4
(Ku et al., 2023, Kim et al., 2018, Verdu et al., 1999)
Act5c>CD2>Gal4
(Jacobs et al., 2025, Zhang et al., 2013, D'Alterio et al., 2005, Halfar et al., 2001)
Act5c>Cd2>Gal4
(Roth et al., 2018)
Act5c>cd2>GAL4
(di Pietro et al., 2023)
Act<CD2<GAL4
(Mitchell et al., 2008, Lee et al., 2001)
Act<CD2<Gal4
(Manzéger et al., 2021, Barth et al., 2011)
Act>>GAL4
(Malin et al., 2024)
Act>>Gal4
(Babski et al., 2019)
Act>CD2> Gal4
(Allen et al., 2020)
Act>CD2>GAL4
(Wang et al., 2024, Tsuboi et al., 2023, Jouandin et al., 2022, Wang et al., 2022, Douthit et al., 2021, Shen et al., 2021, Anding et al., 2018, Blaquiere et al., 2018, Morrison et al., 2018, Kim et al., 2015, Jia et al., 2014, Wehr et al., 2013, Denton et al., 2012, Jimenez-Sanchez et al., 2012, Kawamori and Yamaguchi, 2011, Li et al., 2010, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Juhász et al., 2008, Grewal et al., 2007, Scott et al., 2007, Scott et al., 2007, Hennig et al., 2006, Ward et al., 2006, Ward and Berg, 2005, Frei and Edgar, 2004, Lee and Treisman, 2001, Miron et al., 2001, Kopp et al., 1999, Rangarajan et al., 1999)
Act>CD2>Gal4
(Geng et al., 2025, Wen et al., 2024, Cachoux et al., 2023, Socha et al., 2023, Chatterjee et al., 2022, Gerlach et al., 2022, Hu et al., 2022, Banreti and Meier, 2020, Gupta and Stocker, 2020, Ding et al., 2017, Escobar et al., 2015, Kohlmaier et al., 2015, Nagy et al., 2014, Greer et al., 2013, Faradji et al., 2011, Wu et al., 2009, Narbonne-Reveau et al., 2008, Juhász et al., 2007, Murakami et al., 2007, Datar et al., 2006, Vereshchagina and Wilson, 2006, Frei et al., 2005, Huang et al., 2005, Wang et al., 2005, Wittwer et al., 2005, Bateman and McNeill, 2004, de la Cova et al., 2004, Besse and Pret, 2003, Johnston and Sanders, 2003, Stocker et al., 2003, Besse et al., 2002, Xin et al., 2002, Weihe et al., 2001, Entchev et al., 2000, Chao and Nagoshi, 1999, Larkin et al., 1999, Lawrence et al., 1999)
Act>Cd2>Gal4
(Britton et al., 2002)
Act>FLPout>GAL4
(Rangarajan et al., 2001, Rangarajan et al., 1999)
Act>STOP>GAL4
(Ku and Bilder, 2025)
Act>cd2>GAL4
(Saucedo et al., 2003)
Act>cd2>Gal4
(Andersen et al., 2010, Andersen and Tapon, 2008)
Act>y+>Gal4
(López-Gay et al., 2020)
ActFRTcdc2FRTGal4
(Sotillos and Campuzano, 2000)
Actin 5c>CD2>Gal4
(Prober and Edgar, 2002, Prober and Edgar, 2000)
Actin-FRT-CD2-FRT-Gal4
(Rujano et al., 2017)
Actin-FRT-Stop-FRT-GAl4
(Yoon et al., 2023)
Actin-Gal4
(Ulmschneider et al., 2016)
Actin-flp-out-Gal4
(Baumgartner et al., 2010)
Actin5C > CD2 > GAL4
(Hipfner et al., 2002)
Actin5C >CD2> GAL4
(Pallavi et al., 2006)
Actin5C-FRT-CD2-FRT-Gal4
(Paul et al., 2024)
Actin5C-FRT-stop-FRT-Gal4
(Somogyi and Rorth, 2004)
Actin5C>CD2>GAL4
(Loo et al., 2005, Bhandari and Shashidhara, 2001, Tulina and Matunis, 2001)
Actin5C>CD2>Gal4
(Sustar and Schubiger, 2005, Perrin et al., 2003, Johnston et al., 1999)
Actin5C>y>GAL4
(Xi et al., 2003)
Actin5c > CD2 > Gal4
(Sheng et al., 2009)
Actin5c-Gal4
(Hendricks and Liebl, 2024)
Actin5c>CD2>GAL4
(Usha and Shashidhara, 2010)
Actin5c>CD2>Gal4
(Duman-Scheel et al., 2002)
Actin>CD2>GAL4
(Zoranovic et al., 2018, Small et al., 2014, Monk et al., 2010, Arsham and Neufeld, 2009, Tsai et al., 2008, Ahmad and Baker, 2002, Chern and Choi, 2002, Keisman and Baker, 2001, Gong et al., 1999)
Actin>CD2>Gal4
(Destefanis et al., 2022, Tyra et al., 2020, Greer et al., 2013, Nagy et al., 2013, Takáts et al., 2013, Sen et al., 2010, Bejarano and Milán, 2009, Chen and Schupbach, 2006, Voas and Rebay, 2003, Milan et al., 2002, Milan et al., 2001, Certel et al., 2000, Pignoni and Zipursky, 1997)
F.act>CD2>Gal4
(Aguirre-Tamaral et al., 2022)
GAL4-Act5C(FRT.CD2)
(Laiouar et al., 2020)
Gal4-Act5C(FRT.CD2)
(Bailetti et al., 2019)
P(w+mC=GAL4-Act 5C(FRT.CD2).P)
(Rogers et al., 2005)
P<up>Actin>CD2>GAL4; w+</up>
(Lim et al., 2005)
PGAL4-Act5C(FRT.CD2).P
(Pavlopoulos et al., 2001)
P[Act5c>CD2>GAL4]
(Christiansen et al., 2013)
P[Actin>CD2>Gal4; w+]
(Cho et al., 2000)
P[Actin>CD2>Gal4]
(Parker et al., 2002)
P[GAL4-Act5C(FRT.CD2).P]
(Javeed et al., 2015, Singari et al., 2014)
P[w+,Act>CD2>GAL4]
(Raisin et al., 2003)
Pact > CD2 > gal4
(Portela et al., 2010)
P{GAL4-Act5C (FRT.CD2).P}
(Nakazawa et al., 2012)
P{GAL4-Act5C(FRT.CD2).P}
(Neal et al., 2022, Hsu et al., 2017, Bilak et al., 2014, Mondal et al., 2014, Nandi et al., 2014, Lim and Kelley, 2012, Pignoni and Zipursky, 1997, FlyBase, 1996-)
P{GAL4-Act5C.FRT.P}
(Hartl et al., 2013, FlyBase, 1996-)
P{Gal4-ActRc(FRT.CD2).P}
(Rousso et al., 2013)
P{actin>CD2>GAL4}
(Percival-Smith et al., 2005)
P{w+ Act>CD2>Gal4}
(Fabrizio et al., 2003)
ac>CD2>GAL4
(Wang and Kalderon, 2009)
act < FRT-CD2-FRT < Gal4
(Yatsenko et al., 2009)
act > CD2 > GAL4
(Werthebach et al., 2019)
act > CD2 > Gal4
(Enriquez et al., 2018, Bhattacharya and Baker, 2011)
act > CD2 > Gal4 (AG4)
(Yang et al., 2013)
act > cd2 > GAL4
(O'Keefe et al., 2011)
act-FRT-CD2-FRT-GAL4
(Schwinkendorf and Gallant, 2009)
act-FRT-CD2-FRT-Gal4
(Wakabayashi et al., 2016)
act-FRT-cd2-FRT-gal4
(Mora et al., 2018)
act-GAL4
(Hsu et al., 2017)
act-Gal4
(Gaziova et al., 2004)
act-Gal4(FRT.CD2)
(Loudhaief et al., 2023)
act.CD2 (or y+).Gal4
(Xie et al., 2014)
act5C-(FRT.stop)-GAL4
(Wang et al., 2022)
act5C-FRT-CD2-FRT-Gal4
(Papadopoulos et al., 2019)
act5C>CD2>GAL4
(Bai and Montell, 2002)
act5c < CD2 < Gal4
(Beati et al., 2018)
act5c(FRT-CD2)-GAL4
(Chai et al., 2019)
act5c>CD2>GAL4
(Truscott et al., 2014, Wu et al., 2008, Demontis and Dahmann, 2007, Zhang and Kalderon, 2001, Milan and Cohen, 2000)
act5c>CD2>Gal4
(Williams and Horne-Badovinac, 2024, Shen et al., 2008, Dahmann and Basler, 2000, Methot and Basler, 2000, Methot and Basler, 1999)
act<CD2<GAL4
(Sun and Deng, 2005)
act<CD2<Gal4
(Shen and Sun, 2017, Sun and Spradling, 2012, Gallet et al., 2006)
act<FRT-CD2-FRT<Gal4
(Yatsenko et al., 2014, Yatsenko et al., 2007, Schneider et al., 2006, Shcherbata et al., 2004, Deng et al., 2003)
act>*,CD2>Gal4
(Viktorin et al., 2011)
act>CD2,stop>Gal4
(Guo et al., 2016)
act>CD2>G4
(Rodrigues-Campos and Thompson, 2014)
act>CD2>GAL4
(Dark et al., 2024, Bakopoulos et al., 2023, DeSantis et al., 2023, Neal et al., 2022, Blackie et al., 2021, Lodge et al., 2021, Takemura et al., 2021, O'Farrell et al., 2017, Jain et al., 2015, Bandura et al., 2013, Willoughby et al., 2013, Suissa et al., 2011, Zhang et al., 2011, Bhattacharya and Baker, 2009, Li and Baker, 2007, Miura et al., 2006, Centanin et al., 2005, Chu et al., 2002, Dong et al., 2001, Dong et al., 2000)
act>CD2>Gal4
(Brutscher et al., 2025, Du et al., 2022, Kim et al., 2020, Kale et al., 2018, Lee et al., 2018, Kan et al., 2017, Pan et al., 2017, Zhang et al., 2017, Hu et al., 2014, Greer et al., 2013, Pagarigan et al., 2013, Rougeot et al., 2013, Zhang et al., 2013, Kucherenko et al., 2012, Egoz-Matia et al., 2011, Willecke et al., 2011, Jia et al., 2010, Song et al., 2010, Taniue et al., 2010, Zhang et al., 2008, Zhang et al., 2008, Zhang et al., 2006, Becam et al., 2005, Jia et al., 2005, Reis and Edgar, 2004, Wu and Cohen, 2002, Pavlopoulos et al., 2001, Wu and Cohen, 2000, Wu and Cohen, 1999)
act>CD2>gal4
(Tang et al., 2011, Becker et al., 2010)
act>CD2stop>Gal4
(Pitsouli and Delidakis, 2005, Kerber et al., 2001)
act>Gal4
(Guo et al., 2016)
act>STOP>Gal4
(Voutyraki et al., 2023)
act>cd2>GAL4
(Poulton and Deng, 2006)
act>cd2>gal4
(Pagarigan et al., 2013)
act>cd2STOP>gal4
(Daskalaki et al., 2011)
act>hs-CD2>Gal4
(Zirin and Mann, 2004, Casares and Mann, 2000)
act>hsCD2>GAL4
(Dichtel-Danjoy et al., 2009)
act>y+>GAL4
(Khoury and Bilder, 2022)
act>y+>Gal4
(Simon et al., 2021)
actP>CD2>GAL4
(Cho et al., 2015)
actP>CD2>Gal4
(Cho et al., 2022, Luo et al., 1999)
actin > hsCD2 > Gal4
(Bessa et al., 2002)
actin flip-out
(Manning et al., 2018)
actin-FRT-CD2-FRT-Gal4
(Beam and Moberg, 2010)
actin-FRT-Stop-FRT-Gal4
(Pech et al., 2013)
actin-FRT-stop-FRT-Gal4
(Zipper et al., 2025)
actin-FRT-stop-FRT-gal4
(Wreden et al., 2017)
actin-FRT.stop-Gal4
(Marshall and Heckscher, 2022)
actin-FRT<CD2>FRTGal4
(Yatsenko et al., 2009)
actin-GAL4(FRT)
(Brown et al., 2020)
actin.FRT.CD2.FRT.Gal4
(Lucas et al., 2013)
actin5C = CD2+ = Gal4
(Erkner et al., 1999)
actin5C<CD2<Gal4
(Poulton et al., 2011, Ho et al., 2005)
actin5C>CD2>GAL4
(Dobens et al., 2001)
actin5C>CD2>Gal4
(Cinnamon et al., 2016, Wang and Jiang, 2004)
actin5C>CD2>gal4
(Garrido et al., 2015)
actin5c>CD2>Gal4
(Sun et al., 2020, Montagne et al., 2010, Jia et al., 2002, Burke et al., 1999)
actin<CD2<Gal4
(González-Méndez et al., 2017, Wang et al., 2000)
actinP>CD2>GAL4
(Smith et al., 2015)
actinP>cd2>gal4
(Bohm et al., 2010, Bohm et al., 2010)
actin>>CD2>>Gal4
(Ren et al., 2018)
actin>CD2>GAL4
(Jia et al., 2003, Dobens et al., 2000, Dominguez, 1999, Jarecki et al., 1999)
actin>CD2>Gal4
(Callejo et al., 2011, Smith et al., 2011, Callejo et al., 2006, Mirkovic and Mlodzik, 2006, Collins and Cohen, 2005, Das et al., 2004, Rosin et al., 2004, Torroja et al., 2004, Milan and Cohen, 2003, Peri et al., 2002, Amanai and Jiang, 2001, Hasson et al., 2001)
actin>CDC2>GAL4
(Sapir et al., 2005)
actinGAL4
(Yoshida et al., 2004)
actinP>hsCD2>Gal4
(Bras-Pereira et al., 2006)
flip-out Gal4
(Poulton et al., 2011)
pAct<CD2<Gal4
(Papp et al., 2016)
p{wmC = Gal4-Act5c(FRT.CD2).P}S
(Sheng et al., 2009)
Secondary FlyBase IDs
    References (390)