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General Information
Symbol
TI{T-GEM}
FlyBase ID
FBtp0099367
Feature type
engineered_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
GAL4 gene trap
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Description

The Trojan-Gal4 expression module (T-GEM) is an attP cassette which contains a Trojan GAL4 exon (composed of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an Hsp70 transcription termination signal) followed by a loxP cassette containing the Disc\RFP3xP3.PB marker. Integration of this cassette into a coding intron of a native Drosophila gene of interest using CRISPR/Cas9 technology can result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-GAL4 open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the GAL4 open reading frame. Thus GAL4 should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (5)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TI{T-GEM}
Secondary FlyBase IDs
    References (9)