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General Information
FlyBase ID
Feature type
Component Allele(s)
Also Known As
TDC2-GAL4, dTdc2-Gal4, Tdc-Gal4, Tdc2 GAL4
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
CV term
Qualifiers and info
Construct components
Product class / Tool use(s)
Component allele Scer\GAL4Tdc2.PC
Product class / Tool use(s)
Regulatory region(s)

A Tdc2 promoter (-3459 to +4530 relative to the coding start site) drives GAL4 expression. A Hsp70 3' poly(A) sequence flanks the GAL4 coding region.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Segments and Size
Total Size
Left end
Right end
CV term
Qualifiers and info
Component Alleles
(Wong et al., 2021, Harrigan et al., 2020, Imura et al., 2020, Lee et al., 2020, Palazzo et al., 2020, Pop et al., 2020, Rallis et al., 2020, Sabandal et al., 2020, Sherer et al., 2020, Yalgin et al., 2020, Babski et al., 2019, Castells-Nobau et al., 2019, Chen et al., 2019, Dhiman et al., 2019, Kamimura et al., 2019, Masuzzo et al., 2019, Meissner et al., 2019, Ni et al., 2019, Sapiro et al., 2019, Schützler et al., 2019, Bai et al., 2018, Claßen and Scholz, 2018, Hartenstein et al., 2018, Koon et al., 2018, O'Sullivan et al., 2018, Pauls et al., 2018, Sonn et al., 2018, Strother et al., 2018, Valadas et al., 2018, Chng et al., 2017, Kim et al., 2017, Kurz et al., 2017, Liu et al., 2017, Sethi and Wang, 2017, Søvik et al., 2017, Watanabe et al., 2017, Freyberg et al., 2016, Gupta et al., 2016, Ma et al., 2016, Matsuo et al., 2016, Rohwedder et al., 2016, Wang et al., 2016, Yilmazer et al., 2016, Yu et al., 2016, Kirkhart and Scott, 2015, Okamoto and Nishimura, 2015, Shankar et al., 2015, Shih et al., 2015, Slater et al., 2015, Wasserman et al., 2015, Andrews et al., 2014, Garaulet et al., 2014, Grygoruk et al., 2014, Hess-Homeier et al., 2014, Kairamkonda and Nongthomba, 2014, Kayser et al., 2014, Kunst et al., 2014, Lacin et al., 2014, Lin et al., 2014, Oh et al., 2014, Rezával et al., 2014, Schoofs et al., 2014, Selcho et al., 2014, Vogt et al., 2014, Williams et al., 2014, Williams et al., 2014, Yuan et al., 2014, Castellanos et al., 2013, Chen et al., 2013, Huang et al., 2013, Newquist et al., 2013, Pech et al., 2013, Rubinstein and Wolfner, 2013, Wu et al., 2013, Zhang et al., 2013, Burke et al., 2012, Henry et al., 2012, Koon and Budnik, 2012, Liu et al., 2012, Liu et al., 2012, Majumdar et al., 2012, Marella et al., 2012, Schneider et al., 2012, Selcho et al., 2012, Zhou et al., 2012, Chiang et al., 2011, Hampel et al., 2011, Han et al., 2011, Kahsai and Winther, 2011, Keegan et al., 2011, Kolodziejczyk and Nässel, 2011, Koon et al., 2011, Stavropoulos and Young, 2011, Boerner and Duch, 2010, Busch and Tanimoto, 2010, Certel et al., 2010, Crocker et al., 2010, Pauls et al., 2010, Sitaraman et al., 2010, Yarali and Gerber, 2010, Busch et al., 2009, Dankert et al., 2009, Honjo and Furukubo-Tokunaga, 2009, Lee et al., 2009, Crocker and Sehgal, 2008, Johard et al., 2008, Kaplan et al., 2008, Vömel and Wegener, 2008, Zhou et al., 2008, Brembs et al., 2007, Hardie et al., 2007, Schroll et al., 2006, Cole et al., 2005)
Molecular data

A Tdc2 promoter (-3459 to +4530 relative to the coding start site) drives GAL4 expression. A Hsp70 3' poly(A) sequence flanks the GAL4 coding region.

Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Tissue/Position (including subcellular localization)
motor neuron | subset of adult abdominal segment 6

Comment: innervating abdominal 6 dorsal muscle 133

Additional Information

Drives expression in a subset of octopaminergic and tyraminergic neurons.

FLP/FRT intersectional strategy shows expression of Scer\GAL4Tdc2.PC in a subset of cell bodies in the posterior part of the thoracico-abdominal ganglion when combined with dsxKI.FLPG5. On average nine neurons are labeled in females and three in males. In females these project to the spermathecum, the seminal receptacle, the female accessory gland, the uterus and to both the common and lateral oviduct. In males projections lead to the testis, the ejaculatory duct and the male accessory gland. In both male and female expression can also be detected in a motor neuron that innervates abdominal 6 dorsal muscle 133.

Scer\GAL4Tdc2.PC drives expression in 44 neurons of the ventral nerve cord. In brain hemispheres labeling is observed in three distinct locations with one neuron located basomedial to the calyx, a cluster of four anterior to the vertical mushroom body lobes and around two cells dorsomedial to the antennal lobe. Scer\GAL4Tdc2.PC is detected in clusters of VM neurons in all three larval gnathal neuromeres and in one cell at the margin of the subesophageal ganglion to the ventral nerve cord. All Scer\GAL4Tdc2.PC-expressing neurons are tyraminergic, whereas labeled cells positioned in the subesophageal ganglion are octopaminergic as well.

Scer\GAL4Tdc2.PC drives expression in motor neurons that form type II terminal buttons at the neuromuscular junction.

Scer\GAL4Tdc2.PC drives expression in the adult octopaminergic neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals between the mushroom body alpha and beta lobes labelled with Ecol\lexAL5076 or Ecol\lexAL5251 and Scer\GAL4Tdc2.PC are observed sporadically in the alpha and beta lobes and in the calyx.

Scer\GAL4Tdc2.PC drives expression in clusters of neurons in the adult brain and thoracico-abdominal ganglion. Co-localisation with Tbh protein in the brain is observed in the octopaminergic AL2 cluster, in the G3b and 3 ventral clusters (VMI to VMIII). In the thoracico-abdominal ganglion, 37 of the 39 Tbh-positive neurons also express Scer\GAL4Tdc2.PC.

Expression observed in neurons that innervate the antennal lobes, mushroom body gamma lobes and their spurs, the subesophageal ganglion, the areas surrounding the esophagus, and the mushroom body calyces.

Expression of Scer\GAL4Tdc2.PC is in both octopaminergic and tyraminergic neurons.

The octopaminergic neurons VPM1 and VUM labelled with Scer\GAL4Tdc2.PC co-localise with male-specific fru protein (FruM).

Scer\GAL4Tdc2.PC drives expression in several clusters of cells in the adult, in a total of 137 cells per hemisphere. These clusters include the octopaminergic neurons VM (27 cells), AL2 (7 cells), VL (2 cells) and ASM (8 cells). In addition, Scer\GAL4Tdc2.PC labels cells around the protocerebral bridge (PB1 cluster, 65 cells), ventral to the protocerebral bridge (PB2 cluster, 2 cells), posterior superior medial protocerebrum (PSM cluster, 5 cells) and at the anterior margin of the antennal lobe (AL1 cluster, 1 cell).

Expression is seen in discrete unilateral subsets of cells located along the ventral medial line of the brain, as well as in discrete

bilateral clusters of cells in the lateral protocerebrum region and surrounding the esophagus cavity.

In larva, Scer\GAL4Tdc2.PC drives expression in a few processes that invade the calyx of the mushroom body and in neurites that surround the peduncle.

Scer\GAL4Tdc2.PC drives expression in adult thoracic and abdominal neuromeres. Their cell bodies are located at the midline extending bilateral symmetric axons as well as a net of finer processes.

Reporter construct used in assay
Progenitors and Descendants
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (22)
Reported As
Symbol Synonym
dTdc2(tyrosine decarboxylase2)-Gal4
tyrosine decarboxylase 2 GAL4
Secondary FlyBase IDs
  • FBtp0056985
  • FBtp0021216
  • FBtp0021220
  • FBtp0087282
References (116)