Mi{Trojan-lexA::GAD.0} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 0 'Trojan lexA::GAD ' cassette, where the cassette consists of a splice acceptor site followed by the T2A peptide, sequence encoding a lexA::GAD driver and an Hsp70 transcription termination signal. Integration of the cassette into a Mi{MIC} insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-lexA::GAD open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the lexA::GAD open reading frame. Thus the lexA::GAD driver should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.