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General Information
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| Term |
TALEN |
ID (Ontology) |
FBcv:0003009 (FlyBase CV) |
| Definition |
Sequence change caused by the cleavage of specific DNA sequences by transcription activator-like effector nucleases (TALENs). TALEN proteins comprise a DNA binding domain fused to the catalytic domain of the FokI endonuclease. The DNA binding domain contains a repetitive region within the middle of the protein, consisting of tandem repeats of 34 amino acid segments, with each of these segments recognizing a single base pair. The specificity of the binding is determined by two hypervariable amino acids in positions 12 and 13 (repeat-variable diresidues). Cleavage requires dimerization of FokI. The endonuclease is directed to a target region by a pair of TALENs which bind to adjacent sequences separated by a spacer region, allowing dimerization and promoting cleavage.[ PubMed:10954606 PubMed:20660643 ] |
| Also Known As |
"change induced by TALE endonuclease cleavage" |
| Comment |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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| Data Class | Field | Records |
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| Aberrations (FBab) | MUTAGEN | 3 | | Alleles (FBal) | MUTAGEN | 104 |
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Aberrations | Alleles |
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TALEN (all annotations which use CV term, excluding "NOT" statements) | 3 | 104 |
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Relationships