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General Information
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| Term |
split fluorescent protein |
ID (Ontology) |
FBcv:0005052 (FlyBase CV) |
| Definition |
Non-fluorescent fragment of a fluorescent protein. A functional fluorophore can be reconstituted if a split fluorescent protein fragment is expressed with a complementary split fluorescent protein fragment and the fragments are brought together in vivo, permitting the study of biological interactions. Protein-protein interactions can be studied using the bimolecular fluorescence complementation (BiFC) technique, where a fluorescent protein is reconstituted if the two complementary split fluorescent protein fragments are fused to interacting proteins or protein domains that bring the split halves together into the same macromolecular complex. Associations between closely apposed cells can be studied if the two complementary split fluorescent protein fragments are tethered to the surface of these cells, for example to study connectivity in the nervous system using the GFP Reconstitution Across Synaptic Partners (GRASP) technique.[ PubMed:11983170 PubMed:18255029 ] |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Experimental Tools |
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split fluorescent protein (all annotations which use CV term, excluding "NOT" statements) | 29 | encodes tool | split fluorescent protein | 14 | tagged with | split fluorescent protein | 25 |
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Relationships