FB2025_05 , released December 11, 2025
FB2025_05 , released December 11, 2025
CV Term Report
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General Information
Term protein cleavage tag ID (Ontology) FBcv:0005057 (FlyBase CV)
Definition Sequence that forms part of the protein product encoded by a transgenic locus or modified endogenous locus and which allows the tagged protein product to be cleaved (broken into smaller molecules by the rupture of a covalent bond) by a specific process. Examples of protein cleavage tags include sequences recognized and cleaved by proteases such as the human rhinovirus (HRV) 3C protease (often produced with the tradename PreScission Protease) and the tobacco etch virus (TEV) protease. Protein product cleavage can be used for a number of different purposes, including release of a protein after purification, or removal of a detection tag to assess that the tag does not interfere with protein function.
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  gene product engineering tool
   |__gene product cleavage tag
       |__protein cleavage tag  5 rec.
           |__protease target site 3 rec.
           |   |__conditional protease target site(+) 1 rec.
           |__small molecule-regulated protein cleavage tag 2 rec.
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