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General Information
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| Term |
enhancer trap |
ID (Ontology) |
FBcv:0005069 (FlyBase CV) |
| Definition |
An enhancer trap cassette is designed to report or utilize the expression of endogenous enhancer elements upon integration of the cassette into the genome. The basic components of an enhancer trap cassette are a minimal promoter element fused upstream of a gene. Upon integration into the genome, a neighboring genomic enhancer may activate expression of the gene encoded by the enhancer trap cassette. Depending on the nature of this gene, the insertion may directly report the expression pattern of the 'trapped' enhancer (e.g. if the gene encodes a reporter enzyme or fluorescent protein), or it may be used to drive expression of any gene of interest (e.g. if the gene encodes a driver that forms part of a binary expression system). An enhancer trap cassette may be inserted into a genome as part of a transgenic construct via transposable-element-mediated transgenesis, or may be inserted directly into a modified endogenous locus via a genome engineering method such as homologous recombination or CRISPR. An enhancer trap insertion can be mutagenic, disrupting the function of an endogenous locus, depending on the particular insertion site.[ FlyBase:FBrf0128252 FlyBase:FBrf0216478 PubMed:10899970 ] |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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| Data Class | Field | Records |
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| Constructs (FBtp) | USES | 4 |
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Alleles | Constructs |
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enhancer trap (all annotations which use CV term, excluding "NOT" statements) | 2 | 117 |
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Relationships