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General Information
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| Term |
docking element |
ID (Ontology) |
FBcv:0007044 (FlyBase CV) |
| Definition |
A docking element is an element that has been introduced into a site in the genome to allow integration of DNA at a defined location; this neutralizes the problem of position effects that are seen with non-specific insertion techniques such as transposable-element-mediated transgenesis. A docking element contains a single target site for an integrase, allowing subsequent integration of DNA into this site from a donor element via integrase mediated recombination. The docking element in the genome typically contains the 'attB' site and the donor plasmid contains the 'attP' site, since this directionality gives higher integration rates compared to the alternative combination where the 'attB' site is integrated into the genome. A docking element insertion is typically selected to be 'benign' (that is it does not result in disruption of an endogenous locus) and to show a high rate of integration in the presence of integrase.[ FlyBase:FBrf0192974 FlyBase:FBrf0194996 FlyBase:FBrf0231034 PubMed:11359900 PubMed:12563279 ] |
| Also Known As |
"docking site element" ; "landing site element" |
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Links to External Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Constructs |
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docking element (all annotations which use CV term, excluding "NOT" statements) | 15 |
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Relationships