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Gene Dmel\LIMK1

General Information
Symbol	Dmel\LIMK1	Species	D. melanogaster
Name	LIM-kinase1	Annotation symbol	CG1848
Feature type	protein_coding_gene	FlyBase ID	FBgn0041203
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	Dlimk, LIMK, DLIMK1
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	11B2-11B2	Sequence location	X:12,478,418..12,486,226 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene LIM-kinase1 is referred to in FlyBase by the symbol Dmel\LIMK1 (CG1848, FBgn0041203). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: synapse assembly; neuron projection morphogenesis; regulation of axonogenesis; compound eye development; establishment of planar polarity; imaginal disc morphogenesis; establishment of imaginal disc-derived wing hair orientation; protein phosphorylation; synaptic growth at neuromuscular junction. 19 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; thoracic segment; adult antennal lobe; appendage segment; adult; mushroom body medial lobe; protocerebrum; synaptic neuropil block. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: PDZ domain; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Zinc finger, LIM-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult hindgut, larval/adult salivary gland, larval trachea, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1641452_a_at completely aligns to an exonic region of only a subset of 2 of the 5 FlyBase-annotated transcript isoforms of LIMK1. Gene sequence location is X:12478418..12486226.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 11B2-11B2   Limits computationally determined from genome sequence between P{EP}CG11138EP1313 and P{EP}EP1632&P{EP}EP1449
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\LIMK1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated. DGC clone GM09639 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) LIMK1-RA FBtr0073659  NM_167326   4964   1235 LIMK1-RC FBtr0073660  NM_078584   5627   1257 LIMK1-RD FBtr0073661  NM_167327   4814   1052 LIMK1-RE FBtr0301830  NM_001169262   4520   1235 LIMK1-RF FBtr0301831  NM_001169263   4187   1043
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein LIMK1-PA   FBpp0073492   136.7   1235   8.84     NP_727621   AAF48176 LIMK1-PC   FBpp0073493   139.3   1257   8.76     NP_511139   AAN09311 LIMK1-PD   FBpp0073494   117.1   1052   9.88     NP_727622   AAN09312 LIMK1-PE   FBpp0291044   136.7   1235   8.84     NP_001162733   ACZ95268 LIMK1-PF   FBpp0291045   115.9   1043   9.59     NP_001162734   ACZ95269
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) PDZ domain (IPR001478) Zinc finger, LIM-type (IPR001781) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440058   AA441464   AA735722   AA979204   AE014298 AAF48176   AE014298 AAN09311   AE014298 AAN09312   AB042816 BAB17233   AB042818 BAB17234   AE014298 ACZ95268   AE014298 ACZ95269   AI404032   AI945139   AW940913   AW942338   AY060966 AAL28514   AY119587 AAM50241   BE976632   BF487093   BF487809   BF488996   BF490696   BF492113   BF499666   BF501855   BF506461   BG638112   BG638714   BI167828   BI233030   BI358627   BI580066   BI580531   BT099664   BT133248   CK658197   CL526041   CO187419   CO190778   CO273098   CO276155   CO279146   CO287275   CO304515   CO308448   CO344710   EC052526   EC053528   EC054014   EC054512   EC055359   EC055788   EC089113   EC210100   EC217936   EC218367   EC218544   EC220331   EC234690   EC256339   EV586602
UniProtKB/Swiss-Prot	Q8IR79
UniProtKB/TrEMBL	E1JJM7 E1JJM8 Q95S42
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 2 -- 5   organism | ubiquitous & uniform   (Ohashi et al., 2000) Comment:decreasing with time embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult hindgut, larval/adult salivary gland, larval trachea, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    190.025 Larval Midgut    62 Larval Hindgut    79.1 Larval Malpighian Tubules    52.9 Larval Fat Body    43.7 Larval Salivary Gland    104.1 Larval Trachea    109.55 Larval Carcass    96.875 Adult Head    109.8 Adult Eye    120.375 Adult Brain    386.6 Adult Thoracic-Abdominal Ganglion    318.7 Adult Crop    110.9 Adult Midgut    94.9 Adult Hindgut    116.1 Adult Malpighian Tubules    40.9 Adult Fat Body    76.5 Adult Salivary Gland    189.1 Adult Heart    72.575 Adult VirginFemale Spermatheca    89.4 Adult InseminatedFemale Spermatheca    95.4 Adult Ovary    159.3 Adult Testis    12.2 Adult Male Accessory Gland    77.5 Adult Carcass    81.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    109.8   NA  Eye    120.375   NA  Brain    386.6   190.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    318.7   NA  Crop    110.9   62  Midgut    94.9   79.1  Hindgut    116.1   52.9  Malpighian Tubules    40.9   43.7  Fat Body    76.5   104.1  Salivary Gland    189.1   NA  Heart    72.575   109.55  Trachea    NA   NA  VirginFemale Spermatheca    89.4   NA  InseminatedFemale Spermatheca    95.4   NA  Ovary    159.3   NA  Testis    12.2   NA  Male Accessory Gland    77.5   96.875  Carcass    81.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    8 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    9 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    21 ovary, mated 4-day female    17 testis, mated 4-day male    25 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    18 embryo 04-06hr    10 embryo 06-08hr    11 embryo 08-10hr    13 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    12 white prepupae 24hr    15 pupae 2d postWPP    14 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    14 adult male 05day    19 adult male 30day    19 adult female 01day    12 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    4 embryonic 1182-4H    5 embryonic GM2    13 embryonic Kc167    13 embryonic S1    9 embryonic S3    5 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    11 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    2 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    14 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD15137 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041203 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1848-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4ap-md544 LIMK1Scer\UAS.T:Ivir\HA1 lethal | heat sensitive, with Scer\GAL4ap-md544 LIMK1ΔNgoMIV.Scer\UAS viable LIMK11 LIMK12 LIMK14 LIMK1EY08757 viable, with Scer\GAL4pnr-MD237 LIMK1GD9586 viable, with Scer\GAL4T80 LIMK1D522A.Scer\UAS Sterility Allele fertile LIMK11 LIMK12 LIMK14 LIMK1EY08757 fertile, with Scer\GAL4T80 LIMK1D522A.Scer\UAS Other Phenotypes Allele cell polarity defective, with Scer\GAL4ap-md544 LIMK1ΔNgoMIV.Scer\UAS neuroanatomy defective, with Scer\GAL4ey-OK107 LIMK1Scer\UAS.T:Ivir\HA1 neuroanatomy defective, with Scer\GAL4SG18.1 LIMK1kd.Scer\UAS.T:Hsap\MYC LIMK1Scer\UAS.T:Hsap\MYC neuroanatomy defective | adult stage | somatic clone, with Scer\GAL4Act.PU LIMK1JF02063 LIMK1Scer\UAS.KI.T:Ivir\HA1 LIMK1Scer\UAS.T:Ivir\HA1 neuroanatomy defective | embryonic stage LIMK1EY08757 neuroanatomy defective | embryonic stage, with Scer\GAL4elav.PLu LIMK1HM05159 LIMK1JF02063 visible, with Scer\GAL4en-e16E LIMK1Scer\UAS.cCa visible, with Scer\GAL4hs.PB LIMK1D522A.Scer\UAS visible, with Scer\GAL4T80 LIMK1D522A.Scer\UAS Phenotype manifest in Allele adult mushroom body, with Scer\GAL4ey-OK107 LIMK1Scer\UAS.T:Ivir\HA1 adult olfactory receptor neuron Or59c | somatic clone, with Scer\GAL4Act.PU LIMK1Scer\UAS.KI.T:Ivir\HA1 LIMK1Scer\UAS.T:Ivir\HA1 LIMK1JF02063 antennal lobe, with Scer\GAL4SG18.1 LIMK1Scer\UAS.T:Hsap\MYC LIMK1kd.Scer\UAS.T:Hsap\MYC antennal lobe glomerulus | ectopic, with Scer\GAL4SG18.1 LIMK1kd.Scer\UAS.T:Hsap\MYC antennal lobe glomerulus DM6, with Scer\GAL4SG18.1 LIMK1kd.Scer\UAS.T:Hsap\MYC antennal lobe glomerulus VA6, with Scer\GAL4SG18.1 LIMK1kd.Scer\UAS.T:Hsap\MYC dorsal mesothoracic disc & actin filament, with Scer\GAL4en-e16E LIMK1Scer\UAS.cCa femur, with Scer\GAL4T80 LIMK1D522A.Scer\UAS intersegmental nerve branch ISNb of A1-7 | embryonic stage LIMK1EY08757 intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4elav.PLu LIMK1HM05159 LIMK1JF02063 leg, with Scer\GAL4hs.PB LIMK1D522A.Scer\UAS leg, with Scer\GAL4T80 LIMK1D522A.Scer\UAS lobe system of mushroom body, with Scer\GAL4ey-OK107 LIMK1Scer\UAS.T:Ivir\HA1 mushroom body medial lobe, with Scer\GAL4ey-OK107 LIMK1Scer\UAS.T:Ivir\HA1 mushroom body vertical lobe, with Scer\GAL4ey-OK107 LIMK1Scer\UAS.T:Ivir\HA1 tibia, with Scer\GAL4T80 LIMK1D522A.Scer\UAS wing, with Scer\GAL4en-e16E LIMK1Scer\UAS.cCa wing, with Scer\GAL4T80 LIMK1D522A.Scer\UAS wing hair, with Scer\GAL4ap-md544 LIMK1ΔNgoMIV.Scer\UAS wing vein, with Scer\GAL4en-e16E LIMK1Scer\UAS.cCa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of LIMK1 Class Mutagen Stocks Known lesion LIMK1EY08757 P-element activity 1 -- LIMK11 P-element activity 0 Yes LIMK12 P-element activity 0 Yes LIMK14 0 Yes LIMK1E591 0 --
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of LIMK1 Class Mutagen Stocks Known lesion LIMK1GD9586 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes LIMK1Scer\UAS.KI.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 2 Yes LIMK1Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes LIMK1dsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 1 Yes LIMK1HMJ02235 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes LIMK1JF02063 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes LIMK1D522A.Scer\UAS in vitro construct - regulatory fusion 0 Yes LIMK1GMR.PV in vitro construct - regulatory fusion 0 Yes LIMK1HM05159 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes LIMK1kd.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes LIMK1Scer\UAS.cBa in vitro construct - regulatory fusion 0 Yes LIMK1Scer\UAS.cCa in vitro construct - regulatory fusion 0 Yes LIMK1Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes LIMK1ΔNgoMIV.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)LIMK1-3 (Ang et al., 2006) Df(1)LIMK1-5 (Ang et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9586} NA P{TRiP.HM05159} NA P{TRiP.HMJ02235} NA P{TRiP.JF02063} NA P{UAS-LIMK1.B} NA P{UAS-LIMK1.C} NA P{UAS-LIMK1.D522A} NA P{UAS-LIMK1.IR} NA P{UAS-LIMK1.kd} NA P{UAS-LIMK1.KI} NA P{UAS-LIMK1.MYC} NA P{UAS-LIMK1.T:Ivir\HA1} NA P{UAS-LIMK1.ΔNgoMIV} NA characterization construct P{GMR-LIMK1.V} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}LIMK1EY08757 NA
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
protein serine/threonine kinase activity inferred from direct assay (Ohashi et al., 2000)
Biological Process
CV term Evidence References
compound eye development inferred from genetic interaction with mbt (Menzel et al., 2007) establishment of imaginal disc-derived wing hair orientation inferred from mutant phenotype (Blair et al., 2006) establishment of planar polarity inferred from mutant phenotype (Blair et al., 2006) imaginal disc morphogenesis inferred from mutant phenotype (assigned by UniProtKB) (Chen et al., 2004) neuron projection morphogenesis inferred from mutant phenotype (Sepp et al., 2008) protein phosphorylation inferred from direct assay (Ohashi et al., 2000) regulation of axonogenesis inferred from mutant phenotype (Ng and Luo, 2004) synapse assembly inferred from mutant phenotype (Ang et al., 2006) synaptic growth at neuromuscular junction inferred from mutant phenotype (Ang et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity (Chen et al., 2000) inferred from sequence or structural similarity with UniProtKB:P53667 (FlyBase, 1992-) non-traceable author statement (Ohashi, 2000.5.17) zinc ion binding inferred from electronic annotation with InterPro:IPR001781 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
actin cytoskeleton organization non-traceable author statement (Ohashi, 2000.5.17) protein phosphorylation non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions LIMK1 allele Gene References LIMK1D522A.Scer\UAS br (Chen et al., 2004) Sb (Chen et al., 2004) LIMK1Scer\UAS.cCa bs (Chen et al., 2004) dia (Chen et al., 2004) Pkn (Chen et al., 2004) Rho1 (Chen et al., 2004) RhoGAPp190 (Chen et al., 2004) RhoGEF2 (Chen et al., 2004) rok (Chen et al., 2004) LIMK1Scer\UAS.T:Ivir\HA1 Cdc42 (Ng and Luo, 2004) dia (Ng and Luo, 2004) Pak (Ng and Luo, 2004) pbl (Ng and Luo, 2004) Rac1 (Ng and Luo, 2004) Rho1 (Ng and Luo, 2004) RhoGAPp190 (Ng and Luo, 2004) RhoGEF2 (Ng and Luo, 2004) rok (Ng and Luo, 2004) SCAR (Ng and Luo, 2004) sif (Ng and Luo, 2004) ssh (Niwa et al., 2002, Ng and Luo, 2004) trio (Ng and Luo, 2004) tsr (Ng and Luo, 2004) unspecified Pak (Ang et al., 2006) Pten (Kiger et al., 2003) Rac2 (Ng and Luo, 2004) ssh (Niwa et al., 2002) tsr (Ang et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58605 DroID - A comprehensive database of gene and protein interactions. • FBgn0041203 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32207 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8IR79
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NGH60 Dipteran inclusive ortholog search EOG6J3WQZ Insect inclusive ortholog search EOG68677C Arthropod inclusive ortholog search EOG64F4R0 Metazoa inclusive ortholog search EOG6DJXP5
Orthologs in Drosophila Species (EOG6NGH60)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\LIMK1     Drosophila simulans   Dsim\GD15924 Y   Drosophila sechellia   Dsec\GM11544 Y   Drosophila erecta   Dere\GG19547 Y   Drosophila yakuba   Dyak\GE16209 Y   Drosophila ananassae   Dana\GF20901 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14953 Y   Drosophila persimilis   Dper\GL25348 Y   Drosophila willistoni   Dwil\GK16124 Y   Drosophila virilis   Dvir\GJ16656 Y   Drosophila mojavensis   Dmoj\GI16163 Y   Drosophila grimshawi   Dgri\GH24831 Y
Orthologs in non-Drosophila Dipterans (EOG6J3WQZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000187   Anopheles gambiae Malaria mosquito  Agam\AGAP011710   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000717
Orthologs in non-Dipteran Insects (EOG68677C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49680   Apis mellifera Western honey bee  Amel\LIMK1   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009556   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14235   Atta cephalotes Leafcutter ant  Acep\ACEP18772   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22213   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18102   Linepithema humile Argentine ant  Lhum\LH16411   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19513   Solenopsis invicta Red fire ant  Sinv\SINV17715   Bombyx mori Silkmoth  Bmor\BGIBMGA012778   Pediculus humanus Human body louse  Phum\PHUM255000   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15763
Orthologs in non-Insect Arthropods (EOG64F4R0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\hsp60   Ixodes scapularis Deer tick  Isca\ISCW015511
Orthologs in non-Arthropod Metazoa (EOG6DJXP5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009240gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022207gn   Danio rerio Zebrafish  Drer\limk1   Danio rerio Zebrafish  Drer\limk2   Danio rerio Zebrafish  Drer\ENSDARG00000003022   Xenopus tropicalis Western clawed frog  Xtro\limk1   Xenopus tropicalis Western clawed frog  Xtro\limk2   Gallus gallus Domestic chicken  Ggal\LIMK2   Gallus gallus Domestic chicken  Ggal\LIMK1   Mus musculus House mouse  Mmus\Limk1   Mus musculus House mouse  Mmus\Limk2   Rattus norvegicus Norway rat  Rnor\Limk1   Rattus norvegicus Norway rat  Rnor\Limk2   Homo sapiens Human  Hsap\LIMK1   Homo sapiens Human  Hsap\LIMK2
Human Orthologs (2)
Gene OMIM HGNC Hsap\LIMK1     Hsap\LIMK2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15924 Drosophila sechellia Dsec\GM11544 Drosophila erecta Dere\GG19547 Drosophila yakuba Dyak\GE16209 Drosophila ananassae Dana\GF20901 Drosophila pseudoobscura pseudoobscura Dpse\GA14953 Drosophila persimilis Dper\GL25348 Drosophila willistoni Dwil\GK16124 Drosophila virilis Dvir\GJ16656 Drosophila mojavensis Dmoj\GI16163 Drosophila grimshawi Dgri\GH24831
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	9118 y1 w*; P{UAS-LIMK1.KI}M8 9119 y1 w*; P{UAS-LIMK1.KI}F3 26294 y1 v1; P{TRiP.JF02063}attP2 42576 y1 v1; P{TRiP.HMJ02235}attP40 9116 y1 w*; P{UAS-LIMK1.T:Ivir\HA1}M6 9117 y1 w*; P{UAS-LIMK1.T:Ivir\HA1}F4 17491 y1 w67c23 P{EPgy2}LIMK1EY08757 9240 y1 w*; P{UAS-LIMK1.IR}1.1; P{UAS-LIMK1.IR}2.1/TM3, Sb1
VDRC	v25343 w1118; P{GD9586}v25343 v25344 w1118; P{GD9586}v25344
Genomic Clones ( 2 )
	BACR39P03 BACR48G08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 47 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT04472 GM09639 LD15137
Other clones	FI19304
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT08936 AT14286 AT17428 AT21776 AT22866 AT24679 AT27255 AT28987 GM27789 LP14945 RE08308 RE29303 RE74194 RE74739
Other clones	19031 58027 154790 214473.9 bs09d09 bs54a06 CK01.34B.F7 CK01.61D.F1 CK01.69B.G9 CK01.145D.G5 Dmel_neb_014333_0079_1408 EC052526 EC053528 EC054014 EC054512 EC055359 EC055788 EK029216 EK049621 EK065420 EK109509 EK119827 EK133631 EK245001 EK258211 EP19751 GH23615 LD16409 LD33520
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0041203 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32207
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG1848 LIM-kinase (FlyBase, 1996-)
Additional comments	Source for merge of CG1848 LIM-kinase was sequence comparison (date:001028). (FlyBase, 1996-)
Other Comments
	LIMK1 is a regulator of tsr function and a downstream effector of Pak. (Ang et al., 2006) RNAi experiments show that dsRNA targetted at this gene suppresses the Pten-dsRNA-induced cell shape changes in Kc167 cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078584 NM_167326 NM_167327 NM_001169262 NM_001169263 RefSeq (Proteins) • NP_511139 NP_727621 NP_727622 NP_001162733 NP_001162734 DDBJ / EMBL / GenBank • AA440058 AA735722 AAF48176 AAL28514 AAM50241 AAN09311 AAN09312 AB042816 AB042818 ACZ95268 ACZ95269 AI404032 AI945139 AW940913 AW942338 AY060966 AY119587 BAB17233 BAB17234 CL526041 Entrez Gene - A searchable database of RefSeq genes. • 32207 UniProtKB/Swiss-Prot • Q8IR79 UniProtKB/TrEMBL • E1JJM7 E1JJM8 Q95S42
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) PDZ domain (IPR001478) Zinc finger, LIM-type (IPR001781) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD15137
Linkouts
	BioGRID - A database of protein and genetic interactions • 58605 DroID - A comprehensive database of gene and protein interactions. • FBgn0041203 DRSC - Results from RNAi screens. • FBgn0041203 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041203 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1848-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0041203 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32207 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/limkinase1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32207 modMine - Data generated by the modENCODE project. • FBgn0041203 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8IR79
Synonyms & Secondary IDs ( 25 )
Reported As
Symbol Synonym	CG1848 (Savvateeva-Popova et al., 2003, Morrison et al., 2000, Rogers et al., 2003, Perkins et al., 2009.3.31, Perkins et al., 2009, Ni et al., 2010.12.1, Sepp et al., 2008) Dlimk (Blair et al., 2005, Blair et al., 2003, Chen et al., 2004, Chen et al., 2005, Umemori et al., 2007, Verdier et al., 2006) dLIMK (Ribaya et al., 2002, von Blume et al., 2009) DLIMK (Blair et al., 2002, Niwa et al., 2002, Ohashi et al., 2000, Goold and Davis, 2007) D-Limk (Menzel et al., 2007) D-LIMK1 (Mentzel et al., 2003) DLIMK1 (Ohashi, 2000.5.17, Eaton and Davis, 2005) dLIMK1 (Marques, 2005) LIMK (Lundquist, 2003, Jovceva et al., 2007, Zhang et al., 2011) Limk (Baum, 2002, Ang et al., 2006, Blair et al., 2006, Menzel et al., 2007) limk (Niwa et al., 2002, Vlachos and Harden, 2011) LimK (Stephan et al., 2012, Leiss et al., 2009, Simões et al., 2010) LIMK1 (Hartmann et al., 2011, Miller et al., 2011, Ng, 2008, Sepp et al., 2008) LimK1 (Guenin et al., 2010, Abruzzi et al., 2011) LIMK-1 (Lopatina et al., 2008) LIM-kinase 1 (Edwards et al., 2006)
Name Synonym	LIM-DOMAIN KINASE (Morrison et al., 2000) LIM domain protein kinase (Menzel et al., 2007) LIM kinase (Patel and Van Vactor, 2002, Settleman, 2001, Miller, 2002, Chen et al., 2005) LIM-kinase (Chen et al., 2000, ) Lim Kinase (Dilks and DiNardo, 2007) Lim kinase (Rogers et al., 2003, Rogers et al., 2003, Simões et al., 2010) Lim-kinase (Verdier et al., 2006) LIM-kinase 1 (Edwards et al., 2006) LIM-kinase1 (Ohashi, 2000.5.17, ) LIM Kinase1 (Eaton and Davis, 2005)
Secondary FlyBase IDs
	FBgn0030402
References ( 72 )
Generate a list of	All references relating to this gene ( 72 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 8 ) Review  ( 8 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 11 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 7 )
	Siudeja et al., 2012, PLoS ONE 7(8): e43145 Cofilin/Twinstar phosphorylation levels increase in response to impaired coenzyme a metabolism. [FBrf0219241] Stephan et al., 2012, J. Neurosci. 32(46): 16080--16094 Drosophila Psidin Regulates Olfactory Neuron Number and Axon Targeting through Two Distinct Molecular Mechanisms. [FBrf0220017] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Hartmann et al., 2011, RNA 17(3): 453--468 Distinct regulatory programs establish widespread sex-specific alternative splicing in Drosophila melanogaster. [FBrf0212988] Miller et al., 2011, J. Neurosci. 31(14): 5335--5347 Drosophila mmp2 regulates the matrix molecule faulty attraction (frac) to promote motor axon targeting in Drosophila. [FBrf0213409] Vlachos and Harden, 2011, Genetics 187(2): 501--512 Genetic Evidence for Antagonism Between Pak Protein Kinase and Rho1 Small GTPase Signaling in Regulation of the Actin Cytoskeleton During Drosophila Oogenesis. [FBrf0212983] Zhang et al., 2011, Development 138(3): 455--464 Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis. [FBrf0212692] Show all research papers ...
Recent reviews ( 1 )
	Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...