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BLAST

BLAST

Database Genome Assembly (NT) Annotated genes (NT) Annotated proteins (AA) Intergenic sequences (NT) Syntenic blocks (NT) Transposons - all annotated elements (NT) Transposons - canonical set (NT) GenBank sequences (NT) GenBank EST sequences (NT) GenBank GSS sequences (NT) GenBank protein sequences (AA)

Program blastn: NT -> NT blastp: AA -> AA blastx: NT -> AA tblastn: AA -> NT tblastx: NT -> NT

Sequence file

Sequence Clear sequence

Species (optional)

• All
• Diptera
• Drosophila (genus)
• Sophophora (subgenus)
• Drosophila melanogaster^1,2,3,4
• Drosophila simulans²²
• Drosophila sechellia^6,7
• Drosophila yakuba^6,7
• Drosophila erecta^6,7
• Drosophila ficusphila²¹
• Drosophila eugracilis²¹
• Drosophila biarmipes²¹
• Drosophila takahashii²¹
• Drosophila elegans²¹
• Drosophila rhopaloa²¹
• Drosophila kikkawai²¹
• Drosophila ananassae^6,7
• Drosophila bipectinata²¹
• Drosophila pseudoobscura pseudoobscura^5,6,7
• Drosophila persimilis^6,7
• Drosophila miranda¹⁷
• Drosophila willistoni^6,7
• Drosophila (subgenus)
• Drosophila mojavensis^6,7
• Drosophila virilis^6,7
• Drosophila albomicans¹⁸
• Drosophila grimshawi^6,7

• Musca domestica (House fly)¹⁹
• Glossina morsitans morsitans (Tsetse fly)²⁰
• Culex quinquefasciatus (Southern house mosquito)^6,7
• Aedes aegypti (Yellow fever mosquito)¹³
• Anopheles darlingi (American malaria mosquito)¹⁶
• Anopheles gambiae (Malaria mosquito)^11,12
• Mayetiola destructor (Hessian fly)¹⁶

• Lepidoptera
• Bombyx mori (silkworm)^9,10
• Danaus plexippus (Monarch butterfly)¹⁶

• Coleoptera
• Tribolium castaneum (Red flour beetle)¹⁴

• Hymenoptera
• Nasonia giraulti (Parasitic wasp)¹⁶
• Nasonia longicornis (Parasitic wasp)¹⁶
• Nasonia vitripennis (Parasitic wasp)¹⁶
• Apis mellifera (Western honey bee)⁸
• Apis florea (Dwarf honey bee)¹⁶
• Bombus impatiens (Common eastern bumblebee)¹⁶
• Bombus terrestris (Buff-tailed bumblebee)¹⁶
• Megachile rotundata (Alfalfa leafcutter bee)¹⁶
• Acromyrmex echinatior (Panamanian leafcutter ant)¹⁶
• Atta cephalotes (Leafcutter ant)¹⁶
• Camponotus floridanus (Florida carpenter ant)¹⁶
• Harpegnathos saltator (Jerdon's jumping ant)¹⁶
• Linepithema humile (Argentine ant)¹⁶
• Pogonomyrmex barbatus (Red harvester ant)¹⁶
• Solenopsis invicta (Red fire ant)¹⁶

• Hemiptera
• Acyrthosiphon pisum (Pea aphid)¹⁶
• Rhodnius prolixus (Kissing bug)¹⁶

• Phthiraptera
• Pediculus humanus corporis (Human body louse)¹⁶

• Ixodida
• Ixodes scapularis (Deer tick)¹⁶
• Rhipicephalus microplus (Southern cattle tick)¹⁶

Miss our old phylogenetic tree as much as we do? We hope to reimplement it with this expanded species list soon. In the meantime, we hope you find this version easy to use.

Advanced Settings (optional)

Expect value 0.0001 0.001 0.01 1 10 100 1000

Output format Graphical Alignment View Plain Text Alignment View XML Tabular ASN Text

MegaBLAST (blastn only)

Use legacy BLAST engine

Number of descriptions 0 10 25 50 100 250 500

Number of alignments 0 10 25 50 100 250 500

Low complexity filter

Word size

Matrix PAM30 PAM70 BLOSUM80 BLOSUM62 BLOSUM45

Genetic Codes Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial (4) Invertebrate Mitochondrial (5) Ciliate Nuclear (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15)

Other options (e.g. -G 7 -E 3)