FB2025_01 , released February 20, 2025
Allele: Dmel\NmnatC344S.C345S.EGFP
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General Information
Symbol
Dmel\NmnatC344S.C345S.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0341591
Feature type
allele
Associated gene
Dmel\Nmnat
Associated Insertion(s)
TI{TI}NmnatC344S.C345S.EGFP
Carried in Construct
Key Links
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Li-Kroeger et al., 2018)
Mutagen
gene targeting by homologous recombination
(Li-Kroeger et al., 2018)
CRISPR/Cas9
(Li-Kroeger et al., 2018)
Progenitor genotype
TI{ywing2+}NmnatΔCDS
(Li-Kroeger et al., 2018)
Associated Insertion(s)
TI{TI}NmnatC344S.C345S.EGFP
Cytology
Description

The TI{ywing2+} cassette in TI{ywing2+}NmnatΔCDS has been replaced with sequence encoding Nmnat carrying the amino acid replacements C344S and C345S and tagged internally with EGFP (the EGFP coding sequence is surrounded on each side by a linker sequence).

(Li-Kroeger et al., 2018)
Allele components
Component
Use(s)
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      NmnatC344S.C345S.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)