A TI{CRIMIC.TG4.2} DNA cassette has been inserted into L2HGDH, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CCAAGATCTCGACGTTCTTGCGG. The homology arms of the donor vector overlapped by 6 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.

The PCR check of the insertion gave no band for the left flank and the expected sized product for the right flank.