FB2025_01 , released February 20, 2025
Allele: Scer\GAL4scro-ΔE2-Gal4
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General Information
Symbol
Scer\GAL4scro-ΔE2-Gal4
Species
S. cerevisiae
Name
FlyBase ID
FBal0360583
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{GAL4::p65}scroΔE2-Gal4
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Yoo et al., 2020)
gene targeting by homologous recombination
(Yoo et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{GAL4::p65}scroΔE2-Gal4
Cytology
Description

A 264bp portion of scro exon 2 has been deleted (the deletion is just downstream of the start codon in this exon), and replaced with a sequence cassette that contains the GAL4::p65 coding sequence and a Equa\eqFP578TagRFP.3xP3 marker. This results in GAL4::p65 being expressed under the control of the regulatory sequences of the scro RA, RB and RC transcript isoforms, while expression of the native scro RA, RB and RC transcripts is disrupted due to the presence of an SV40 transcription termination signal downstream of the GAL4::p65 coding sequence. (Expression of the scro RF isoform is not affected by the insertion).

(Yoo et al., 2020)
Allele components
Component
Use(s)
Inserted element
TI{GAL4::p65}
Encoded product / tool
GAL4::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4scro-ΔE2-Gal4
      Name Synonyms
      Secondary FlyBase IDs
        References (2)