FlyBase Allele Report: Dmel\OtopLa[lexA1]

General Information

Symbol

Dmel\OtopLa^lexA1

Species

D. melanogaster

Name

FlyBase ID

FBal0371069

Feature type

allele

Associated gene

Dmel\OtopLa

Associated Insertion(s)

TI{lexA::UnkAD}OtopLa^lexA1

Carried in Construct

Also Known As

OtopLA¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Ganguly et al., 2021)

Progenitor genotype

Associated Insertion(s)

TI{lexA::UnkAD}OtopLa^lexA1

Cytology

Description

Replacement of 40 base pairs at the beginning of the coding sequence for OtopLa isoforms 'c'-'g' with the Ecol\lexA and miniwhite genes. The deletion removes the first 14 amino acids and also changed the reading frame.

(Ganguly et al., 2021)

Allele components

Component

Use(s)

Inserted element

TI{lexA::UnkAD}

promoter trap

Encoded product / tool

lexA::UnkAD

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

X:5,643,026..5,643,065 [-]

Comment:

The first 40 base pairs of the coding sequence common to OtopLa isoforms RC-RG were replaced with

the Ecol\lexA and miniwhite genes.

(Ganguly et al., 2021)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage

(Ganguly et al., 2021)

abnormal proboscis extension reflex

(Ganguly et al., 2021)

abnormal taste perception

(Ganguly et al., 2021)

Phenotype Manifest In

intermediate labellar taste bristle I-b

(Ganguly et al., 2021)

L-type sensillum L7

(Ganguly et al., 2021)

short labellar taste bristle S-b

(Ganguly et al., 2021)

Detailed Description

Statement

Reference

OtopLa^lexA1 adults exhibit decreased repulsion to low pH (HCl, tartaric acid, glycolic acid, citric acid, acetic acid and propionic acid), assessed in proboscis extension response assays; the mild attraction to 0.01mM HCl in binary and proboscis extension response assays is eliminated. Small labellar gustatory sensilla (S-b) S3, S5, S9 and intermediate labellar gustatory sensilla (I-b) I8 show decreased HCL- and tartaric acid-induced action potentials; large labellar gustatory sensilla 7 show decreased HCL-induced action potentials.

(Ganguly et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions